FastQCFastQC Report
Thu 2 Feb 2017
SRR4062921_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062921_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4232295
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT121330.2866766139883916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT71110.16801758856601443No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT57470.13578921129080085No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC55770.1317724780526877No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA52070.12303017629914739No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG50630.11962776696803981No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC50370.11901344306103426No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG46360.10953867818760271No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC45720.10802649626266601No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG44460.10504938809794687No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG42890.10133981681333651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG12150.017.11748328
CTAGCGG12550.016.8280529
AAGACGG15300.016.2056675
TAGCGGC12950.015.93743530
CAAGACG15650.015.8430544
GTATCAA88050.015.5911751
TAGGACG5000.015.3565184
CGCAAGA14300.014.998292
TACCGTC9700.014.5138887
GCGCAAG15150.014.485561
ATACCGT10300.014.4433276
ACCGTCG9750.014.27537258
CGGTCCA14700.014.14979510
GTCCTAC9600.014.0163661
GACGGAC16900.014.0103137
AGCGGCG14850.013.89830131
AGGCCCG13250.013.88692510
CGCTTCG10050.013.690865532
CGACCCG8950.013.58367355
AGACGGA18850.013.4929626
Produced by FastQC (version 0.11.5)