##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062921_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4232295
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25904503348656	32.0	32.0	32.0	32.0	32.0
2	30.888053408375363	32.0	32.0	32.0	32.0	32.0
3	30.935329649752674	32.0	32.0	32.0	32.0	32.0
4	31.00006355889653	32.0	32.0	32.0	32.0	32.0
5	30.919392197377547	32.0	32.0	32.0	32.0	32.0
6	34.5742454153125	36.0	36.0	36.0	32.0	36.0
7	34.50484146308327	36.0	36.0	36.0	32.0	36.0
8	34.49170580028093	36.0	36.0	36.0	32.0	36.0
9	34.62581861614089	36.0	36.0	36.0	32.0	36.0
10	34.3521994567959	36.0	36.0	36.0	32.0	36.0
11	34.60848168664992	36.0	36.0	36.0	32.0	36.0
12	34.43060679843914	36.0	36.0	36.0	32.0	36.0
13	34.51596852298812	36.0	36.0	36.0	32.0	36.0
14	34.420533304034805	36.0	36.0	36.0	32.0	36.0
15	34.36652241868774	36.0	36.0	36.0	32.0	36.0
16	34.381996765348354	36.0	36.0	36.0	32.0	36.0
17	34.307448322954805	36.0	36.0	36.0	32.0	36.0
18	34.3345863178252	36.0	36.0	36.0	32.0	36.0
19	34.3190332904488	36.0	36.0	36.0	32.0	36.0
20	34.304584155877606	36.0	36.0	36.0	32.0	36.0
21	34.291418249436774	36.0	36.0	36.0	32.0	36.0
22	34.268998971007456	36.0	36.0	36.0	32.0	36.0
23	34.21930607389135	36.0	36.0	36.0	32.0	36.0
24	34.204322950077916	36.0	36.0	36.0	32.0	36.0
25	34.17505206985808	36.0	36.0	36.0	32.0	36.0
26	34.134666888768386	36.0	36.0	36.0	32.0	36.0
27	34.1356268880123	36.0	36.0	36.0	32.0	36.0
28	34.12736683052575	36.0	36.0	36.0	32.0	36.0
29	34.091236551327356	36.0	36.0	36.0	32.0	36.0
30	34.06780860029842	36.0	36.0	36.0	32.0	36.0
31	34.08514718373837	36.0	36.0	36.0	32.0	36.0
32	34.03781943366424	36.0	36.0	36.0	32.0	36.0
33	34.002143045321745	36.0	36.0	36.0	32.0	36.0
34	34.008932505886285	36.0	36.0	36.0	32.0	36.0
35	33.970096838712806	36.0	36.0	36.0	32.0	36.0
36	33.93476588942878	36.0	36.0	36.0	32.0	36.0
37	33.938722844225175	36.0	36.0	36.0	32.0	36.0
38	33.335418490440766	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	2.0
7	5.0
8	11.0
9	11.0
10	34.0
11	28.0
12	17.0
13	16.0
14	449.0
15	1080.0
16	1368.0
17	1618.0
18	2101.0
19	2715.0
20	3514.0
21	5293.0
22	7682.0
23	11775.0
24	17743.0
25	26480.0
26	39292.0
27	56293.0
28	79732.0
29	107927.0
30	144922.0
31	195459.0
32	268097.0
33	402915.0
34	908588.0
35	1947127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.0699537269226	17.586300708763495	11.884468711261675	25.459276853052227
2	16.58661410156057	19.48673370167679	37.931936919035756	25.994715277726883
3	19.128119996928003	23.493994836682006	28.37029378924919	29.007591377140795
4	12.74447813867942	15.404999506164438	35.91297435977409	35.93754799538205
5	14.937399034988918	36.17695225012127	33.240735555727255	15.64491315916256
6	34.27102245501483	35.378367803294864	16.79587192163853	13.554737820051773
7	30.61650032263834	30.53155818843355	20.362720633944768	18.489220854983337
8	28.107710264916307	32.768696778095965	19.317129301463986	19.806463655523736
9	27.281669358278094	14.786882263363644	18.885259381161713	39.04618899719655
10	16.314192471561373	26.288622281365658	30.91892487061323	26.478260376459733
11	37.20119729012453	21.554719220910805	22.34000374271305	18.904079746251618
12	25.391327113244383	23.701119110363614	28.089450382909508	22.81810339348249
13	28.90901262563485	19.550025663396433	25.556394722887305	25.984566988081408
14	23.69712986474716	20.176108939523044	25.008282280928352	31.11847891480144
15	24.990812514076648	27.26321160475778	22.50495651571775	25.24101936544782
16	25.77170667986183	26.20489742793584	23.685552478889868	24.337843413312463
17	23.934326739901117	26.19840607189101	25.081384958712565	24.785882229495304
18	24.91124342846935	24.96128972956803	26.374873259750935	23.75259358221168
19	25.370102001200852	25.13817905396704	25.705923593262682	23.785795351569433
20	25.636324669896126	24.525049993183014	25.16218395837316	24.676441378547697
21	26.932228685064658	24.268322536374757	24.77341982242201	24.026028956138575
22	25.658122481540058	24.38973903834144	25.302782300854844	24.649356179263663
23	24.130668463180022	24.268502500412936	26.02055199306411	25.58027704334293
24	24.566410812831588	25.311191945200445	25.50430523052957	24.618092011438396
25	24.780987045322583	24.69142279313328	25.584631246958896	24.94295891458524
26	24.35688896209643	25.727513712037407	26.127395456127744	23.78820186973842
27	25.144903889300966	24.937646844487272	25.153505139365063	24.7639441268467
28	24.201358524021092	25.01128874058297	26.06771637579798	24.719636359597956
29	24.183617528072894	25.18646735226285	25.975155015312488	24.654760104351773
30	24.19221947633172	25.281444587816736	26.216328547229644	24.3100073886219
31	24.908903723268637	25.16861170006765	24.97017410665867	24.95231047000504
32	24.536327391105964	25.135212984227177	24.844052270933055	25.4844073537338
33	24.090646371588402	24.789258504684117	25.705783747585638	25.414311376141846
34	25.025479659162148	24.972901646979647	25.748693170553384	24.252925523304818
35	25.43855897106632	24.82104468642558	25.79626873060889	23.94412761189921
36	24.107384865875257	25.559828862902457	25.33504895792348	24.99773731329881
37	25.29069575104988	25.160234749461	25.191048215512783	24.358021283976335
38	24.26112137011452	25.016948031157437	25.72884804190872	24.993082556819324
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	231.0
1	367.0
2	503.0
3	503.0
4	1210.5
5	1918.0
6	1918.0
7	2403.0
8	2888.0
9	2859.0
10	2830.0
11	2830.0
12	3916.5
13	5003.0
14	7008.0
15	9013.0
16	9013.0
17	13926.0
18	18839.0
19	18839.0
20	23745.0
21	28651.0
22	31588.5
23	34526.0
24	34526.0
25	41554.5
26	48583.0
27	48583.0
28	61683.0
29	74783.0
30	89856.5
31	104930.0
32	104930.0
33	130397.0
34	155864.0
35	155864.0
36	174371.5
37	192879.0
38	216381.5
39	239884.0
40	239884.0
41	254437.0
42	268990.0
43	300092.5
44	331195.0
45	331195.0
46	336416.5
47	341638.0
48	341638.0
49	363994.5
50	386351.0
51	389727.0
52	393103.0
53	393103.0
54	374924.0
55	356745.0
56	356745.0
57	342638.5
58	328532.0
59	295036.0
60	261540.0
61	261540.0
62	245976.5
63	230413.0
64	191398.0
65	152383.0
66	152383.0
67	127522.0
68	102661.0
69	102661.0
70	81890.0
71	61119.0
72	47592.5
73	34066.0
74	34066.0
75	25554.0
76	17042.0
77	17042.0
78	16660.5
79	16279.0
80	12706.5
81	9134.0
82	9134.0
83	9216.0
84	9298.0
85	9298.0
86	6270.5
87	3243.0
88	2809.5
89	2376.0
90	2376.0
91	1765.5
92	1155.0
93	993.5
94	832.0
95	832.0
96	767.0
97	702.0
98	702.0
99	1437.5
100	2173.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12321919903976448
2	0.05590347553750388
3	0.012286478140110742
4	0.0027644575815249173
5	2.8353411092563256E-4
6	2.599062683484965E-4
7	4.725568515427209E-5
8	3.5441763865704066E-4
9	2.3627842577136045E-4
10	5.43440379274129E-4
11	0.0016775768229766593
12	0.0022210172022507886
13	0.013869543592778859
14	0.0087186739109632
15	0.0235333312068275
16	0.010419878576516996
17	0.02036720030149127
18	0.005056358311507114
19	0.0067575629770609096
20	0.0051508696818156575
21	0.005647054375935515
22	0.006946585717677997
23	0.009309369975391602
24	0.013751404379893178
25	0.01644497843368669
26	0.01876050700624602
27	0.008317000587151888
28	0.004772824200581481
29	0.010750668372596901
30	0.002646318368639237
31	0.005528915163049835
32	0.00633226181067246
33	0.007868071578186303
34	0.01153038717764239
35	0.0154053533602927
36	0.014176705546281627
37	0.00895495233673456
38	0.0056706822185126505
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4232295.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.883886673817095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76772982321003	35.522856585637705
2	13.533053312804789	12.689642757365293
3	4.582824305013874	6.445818460868553
4	2.0488478577762543	3.8423180310349934
5	1.0902349533875002	2.5557226001226914
6	0.669786526180964	1.8841317353470766
7	0.4391469883239769	1.4412242343610588
8	0.29880441701023397	1.1207289939795035
9	0.22212564323306128	0.9372702136218815
>10	1.1785727012596239	9.710222406281261
>50	0.07477960402096988	2.4211420062266136
>100	0.06949881325942053	6.921969512950523
>500	0.014018168986483436	4.604308786256514
>1k	0.01022263577813859	8.81418686084683
>5k	3.036426468754036E-4	0.8005822311736349
>10k+	5.060710781256726E-5	0.28787458392595916
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	12133	0.2866766139883916	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	7111	0.16801758856601443	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5747	0.13578921129080085	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	5577	0.1317724780526877	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	5207	0.12303017629914739	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	5063	0.11962776696803981	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	5037	0.11901344306103426	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	4636	0.10953867818760271	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	4572	0.10802649626266601	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	4446	0.10504938809794687	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	4289	0.10133981681333651	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1813921288568022E-4
2	0.0	0.0	0.0	0.0	1.1813921288568022E-4
3	0.0	0.0	0.0	0.0	1.1813921288568022E-4
4	0.0	0.0	0.0	2.3627842577136045E-5	1.1813921288568022E-4
5	0.0	0.0	0.0	2.3627842577136045E-5	1.1813921288568022E-4
6	0.0	0.0	0.0	2.3627842577136045E-5	1.4176705546281628E-4
7	0.0	0.0	0.0	9.451137030854418E-5	1.4176705546281628E-4
8	0.0	0.0	0.0	1.1813921288568022E-4	1.4176705546281628E-4
9	0.0	0.0	0.0	1.8902274061708836E-4	1.4176705546281628E-4
10	0.0	0.0	0.0	1.8902274061708836E-4	1.6539489803995232E-4
11	0.0	0.0	0.0	2.126505831942244E-4	1.6539489803995232E-4
12	0.0	0.0	0.0	2.126505831942244E-4	1.8902274061708836E-4
13	0.0	2.3627842577136045E-5	0.0	2.126505831942244E-4	2.3627842577136045E-4
14	0.0	2.3627842577136045E-5	0.0	2.599062683484965E-4	2.3627842577136045E-4
15	0.0	4.725568515427209E-5	0.0	2.599062683484965E-4	2.3627842577136045E-4
16	0.0	4.725568515427209E-5	0.0	2.8353411092563256E-4	2.3627842577136045E-4
17	0.0	4.725568515427209E-5	0.0	3.3078979607990464E-4	2.3627842577136045E-4
18	0.0	4.725568515427209E-5	0.0	3.544176386570407E-4	2.3627842577136045E-4
19	0.0	4.725568515427209E-5	0.0	3.544176386570407E-4	2.3627842577136045E-4
20	0.0	4.725568515427209E-5	0.0	3.780454812341767E-4	2.3627842577136045E-4
21	0.0	4.725568515427209E-5	0.0	4.489290089655849E-4	2.3627842577136045E-4
22	0.0	4.725568515427209E-5	0.0	6.615795921598093E-4	2.3627842577136045E-4
23	2.3627842577136045E-5	4.725568515427209E-5	0.0	0.001063252915971122	2.8353411092563256E-4
24	2.3627842577136045E-5	7.088352773140814E-5	0.0	0.0017720881932852034	2.8353411092563256E-4
25	2.3627842577136045E-5	7.088352773140814E-5	0.0	0.0021737615170965164	2.8353411092563256E-4
26	2.3627842577136045E-5	7.088352773140814E-5	0.0	0.0028353411092563257	2.8353411092563256E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	1215	0.0	17.117483	28
CTAGCGG	1255	0.0	16.82805	29
AAGACGG	1530	0.0	16.205667	5
TAGCGGC	1295	0.0	15.937435	30
CAAGACG	1565	0.0	15.843054	4
GTATCAA	8805	0.0	15.591175	1
TAGGACG	500	0.0	15.356518	4
CGCAAGA	1430	0.0	14.99829	2
TACCGTC	970	0.0	14.513888	7
GCGCAAG	1515	0.0	14.48556	1
ATACCGT	1030	0.0	14.443327	6
ACCGTCG	975	0.0	14.2753725	8
CGGTCCA	1470	0.0	14.149795	10
GTCCTAC	960	0.0	14.016366	1
GACGGAC	1690	0.0	14.010313	7
AGCGGCG	1485	0.0	13.898301	31
AGGCCCG	1325	0.0	13.886925	10
CGCTTCG	1005	0.0	13.6908655	32
CGACCCG	895	0.0	13.5836735	5
AGACGGA	1885	0.0	13.492962	6
>>END_MODULE