FastQCFastQC Report
Thu 2 Feb 2017
SRR4062921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4232295
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT131650.31106054752799606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT82460.19483518989106383No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC58040.1371359983176976No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT57500.13586009481853226No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC54450.12865360283250576No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA53080.12541658839943814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT51100.1207382755691652No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG49200.11624898547950935No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG47930.11324824947221307No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC47750.11282294830582461No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC47550.1123503914542819No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG47380.11194871813047058No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA43770.10341906696012446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG11900.017.47839729
TCTAGCG12400.016.77362428
TACCGTC9600.016.334717
TAGGACG5600.016.2865074
TAGCGGC12800.016.24944930
TCGCGTG703.6976207E-416.0009699
ATCGTTT9050.015.91106129
GTATCAA88850.015.721831
CATCGTT9550.015.07802128
CGTCGTA9950.014.95497210
ACCGTCG10100.014.8923438
CAAGACG15700.014.8797054
ATACCGT10850.014.7477866
AGCGGCG14450.014.50469731
CGCAAGA14350.014.4946242
GACGGAC15300.014.2234197
AAGACGG15600.014.1550375
CGGTCCA15000.014.08018610
GCGCAAG14700.014.0416661
ATCGCGT700.0068039713.715448
Produced by FastQC (version 0.11.5)