FastQCFastQC Report
Thu 2 Feb 2017
SRR4062920_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062920_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183851
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38560.3257166653573803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26220.2214805748358535No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20170.17037617064985375No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20130.1700382902915992No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17780.15018781924414473No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17130.1446972634225084No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16940.14309233172079933No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16710.1411495196608357No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16610.14030481876519935No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16340.13802412634698116No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15670.13236463034621754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14950.12628278389763575No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG14440.12197480932989034No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14200.11994752718036308No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13550.11445697135872675No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13420.11335886019439947No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13400.11318992001527219No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA12530.10584102222323587No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11990.10127963738679951No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT11950.10094175702854498No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT11880.10035046640159953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTCG851.05610525E-820.7066219
ACACCGT507.210764E-419.1941976
GCCGGTC4600.018.4331048
CGGTCCA4300.018.23320810
CGCCGGT4400.018.1801627
GTATTAG2750.018.0553151
CCGGTCC4900.017.6332829
GGACCGT550.001367445517.449276
GTATTAC753.204809E-517.0845991
GATATAC1252.8139766E-916.6574841
CTTGCGC4950.016.479173
GGTCCAA5300.016.3024711
GTCCTAT5350.016.1665021
CTATTAG703.6670725E-416.0168111
ATACCGT2600.015.9951636
CTAGCGG4950.015.83760829
TAGCGGC4850.015.83494730
ACCGTCG2750.015.707668
CGTCGTA2650.015.69867310
TACCGTC2650.015.6966847
Produced by FastQC (version 0.11.5)