##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062920_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1183851
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.243106607165934	32.0	32.0	32.0	32.0	32.0
2	30.873058349403767	32.0	32.0	32.0	32.0	32.0
3	30.934068560992895	32.0	32.0	32.0	32.0	32.0
4	30.986727214826868	32.0	32.0	32.0	32.0	32.0
5	30.91482796399209	32.0	32.0	32.0	32.0	32.0
6	34.5851648560503	36.0	36.0	36.0	32.0	36.0
7	34.50727583116456	36.0	36.0	36.0	32.0	36.0
8	34.48175150420112	36.0	36.0	36.0	32.0	36.0
9	34.61434758259274	36.0	36.0	36.0	32.0	36.0
10	34.34132842731053	36.0	36.0	36.0	32.0	36.0
11	34.61157274015058	36.0	36.0	36.0	32.0	36.0
12	34.4251717488096	36.0	36.0	36.0	32.0	36.0
13	34.513923627213224	36.0	36.0	36.0	32.0	36.0
14	34.41120039599578	36.0	36.0	36.0	32.0	36.0
15	34.36100573467438	36.0	36.0	36.0	32.0	36.0
16	34.37200289563467	36.0	36.0	36.0	32.0	36.0
17	34.292486132123045	36.0	36.0	36.0	32.0	36.0
18	34.32219510732347	36.0	36.0	36.0	32.0	36.0
19	34.30840874400579	36.0	36.0	36.0	32.0	36.0
20	34.2882752981583	36.0	36.0	36.0	32.0	36.0
21	34.26774315348806	36.0	36.0	36.0	32.0	36.0
22	34.244184445508765	36.0	36.0	36.0	32.0	36.0
23	34.19845149431812	36.0	36.0	36.0	32.0	36.0
24	34.17495360480331	36.0	36.0	36.0	32.0	36.0
25	34.1570298964988	36.0	36.0	36.0	32.0	36.0
26	34.11145068087116	36.0	36.0	36.0	32.0	36.0
27	34.11868469934139	36.0	36.0	36.0	32.0	36.0
28	34.10588917017429	36.0	36.0	36.0	32.0	36.0
29	34.070220830155144	36.0	36.0	36.0	32.0	36.0
30	34.04178904270892	36.0	36.0	36.0	32.0	36.0
31	34.05448320776855	36.0	36.0	36.0	32.0	36.0
32	34.00418549293788	36.0	36.0	36.0	32.0	36.0
33	33.965365573877115	36.0	36.0	36.0	32.0	36.0
34	33.95959035385365	36.0	36.0	36.0	32.0	36.0
35	33.92299030874663	36.0	36.0	36.0	32.0	36.0
36	33.872792268621644	36.0	36.0	36.0	32.0	36.0
37	33.87258531690221	36.0	36.0	36.0	32.0	36.0
38	33.26592113365618	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	5.0
10	7.0
11	16.0
12	4.0
13	4.0
14	148.0
15	400.0
16	416.0
17	495.0
18	573.0
19	785.0
20	1083.0
21	1579.0
22	2372.0
23	3507.0
24	5138.0
25	7760.0
26	11458.0
27	15836.0
28	22468.0
29	29856.0
30	40866.0
31	54624.0
32	75037.0
33	113106.0
34	254246.0
35	542059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79492288591717	17.142702494240545	11.513831144008309	25.54854347583398
2	16.88227170460673	19.71623976700119	37.12987520094119	26.271613327450893
3	18.9625501925756	23.71539531854175	28.48640396860329	28.835650520279366
4	12.482091811565311	15.04820005811714	36.02933226109463	36.44037586922292
5	14.811276447652064	36.34866976165859	33.22951933018428	15.610534460505065
6	34.263520316797425	35.516299389786525	16.7110135760672	13.50916671734885
7	30.597431602456727	30.564657207706038	20.520910148321033	18.317001041516203
8	28.718889587176733	32.12177576851082	19.495915016116932	19.663419628195513
9	27.79588257952034	14.092518634148979	18.570796250870046	39.540802535460635
10	16.21155436471098	25.98595933427208	31.01642357500589	26.786062726011046
11	37.93940008278216	21.090443729251664	21.86242957181352	19.107726616152657
12	25.078390295822	23.797114426179654	28.173962257775674	22.950533020222668
13	29.440399160923995	19.022491663730914	25.396833442737627	26.140275732607464
14	23.95479758258134	19.774832691583132	24.871720357983413	31.398649367852116
15	25.360540263321763	27.012152207176417	22.465149957205778	25.162157572296046
16	26.06761430548257	25.63769626784848	23.57630305969178	24.718386366977157
17	24.148780496047635	25.797231487771167	25.15562742692608	24.898360589255116
18	25.313633810193576	24.26580840270826	26.398459179665227	24.022098607432937
19	25.64345595912699	24.826401208348962	25.762483062502323	23.767659770021726
20	25.984789416285615	23.677436958356317	25.103038484314244	25.234735141043824
21	27.41032147756685	23.95217370736553	24.3176119967832	24.319892818284416
22	25.97732492965254	23.988673446717133	25.121835631505185	24.91216599212514
23	24.119503098244117	23.827545861193595	25.809999704325715	26.242951336236576
24	24.616976788392083	25.063552072993012	25.343527573024687	24.975943565590217
25	24.843871447399547	24.32175323578115	25.412372005001522	25.422003311817782
26	24.506875899248666	25.485393624849724	25.83111064549896	24.17661983040265
27	25.57058222745232	24.552036879502122	24.933790750895252	24.943590142150306
28	24.30259599943571	24.581447782237117	26.04033002670253	25.075626191624643
29	24.137808685779166	25.078480867226254	25.854165821878	24.92954462511658
30	24.27617735481522	25.236459289806973	26.135414282081975	24.351949073295835
31	25.057633029026434	25.056281429958023	24.555851874879096	25.330233666136447
32	24.62186456986118	24.97455162282506	24.555635432250718	25.84794837506304
33	24.299451489375723	24.424731214588203	25.43381167154241	25.842005624493662
34	25.217832806457007	24.750655990592257	25.482425534718928	24.549085668231807
35	26.113344761135558	24.44716105851074	25.48723749918685	23.952256681166855
36	24.39525105328921	25.436670431783103	25.01053922831728	25.157539286610408
37	25.668049848615915	25.259008704584772	24.799027492430795	24.273913954368513
38	24.429877173123383	24.837047424352498	25.489618003345214	25.243457399178904
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	139.0
1	187.0
2	235.0
3	235.0
4	571.5
5	908.0
6	908.0
7	1134.5
8	1361.0
9	1341.0
10	1321.0
11	1321.0
12	1630.0
13	1939.0
14	2509.5
15	3080.0
16	3080.0
17	4508.5
18	5937.0
19	5937.0
20	7101.0
21	8265.0
22	8493.5
23	8722.0
24	8722.0
25	9972.5
26	11223.0
27	11223.0
28	14622.5
29	18022.0
30	22167.5
31	26313.0
32	26313.0
33	33400.0
34	40487.0
35	40487.0
36	44984.0
37	49481.0
38	57219.0
39	64957.0
40	64957.0
41	69251.0
42	73545.0
43	83565.5
44	93586.0
45	93586.0
46	96066.0
47	98546.0
48	98546.0
49	103309.0
50	108072.0
51	109154.5
52	110237.0
53	110237.0
54	104170.0
55	98103.0
56	98103.0
57	94811.5
58	91520.0
59	81704.5
60	71889.0
61	71889.0
62	69673.5
63	67458.0
64	55994.0
65	44530.0
66	44530.0
67	37870.0
68	31210.0
69	31210.0
70	25444.0
71	19678.0
72	15510.0
73	11342.0
74	11342.0
75	8622.0
76	5902.0
77	5902.0
78	5803.0
79	5704.0
80	4524.5
81	3345.0
82	3345.0
83	3344.5
84	3344.0
85	3344.0
86	2240.5
87	1137.0
88	977.5
89	818.0
90	818.0
91	598.0
92	378.0
93	324.0
94	270.0
95	270.0
96	250.5
97	231.0
98	231.0
99	423.5
100	616.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12222821959858123
2	0.05651048991807246
3	0.013515214330181755
4	0.002956453134727259
5	2.534102686909079E-4
6	2.534102686909079E-4
7	0.0
8	2.534102686909079E-4
9	2.534102686909079E-4
10	3.3788035825454385E-4
11	0.001773871880836355
12	0.0026185727764727147
13	0.01419097504669084
14	0.00920723976243632
15	0.02365162507781807
16	0.011572402270218128
17	0.021539872838727173
18	0.005575025911199974
19	0.007348897792036328
20	0.005912906269454518
21	0.00565949600076361
22	0.00709548752334542
23	0.009798530389381771
24	0.01486673576319993
25	0.017823188897927188
26	0.018921300062254457
27	0.008784889314618141
28	0.005743966090327245
29	0.010389821016327225
30	0.002703042866036351
31	0.005743966090327245
32	0.006926547344218149
33	0.008447008956363597
34	0.012248162986727215
35	0.01588037683796356
36	0.015035675942327202
37	0.009038299583309048
38	0.005997376359018153
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1183851.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.02102801608678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.11520474969332	41.676753107983245
2	12.16020315315136	12.651725378227873
3	3.4704431535489904	5.416080615570257
4	1.358906996582886	2.827669557619786
5	0.7190778734879433	1.8703585101232199
6	0.4193464342396995	1.3088899562417697
7	0.2980353530648779	1.0852873816100628
8	0.1979844489744737	0.823948365348048
9	0.1583827189480883	0.741530867369625
>10	0.8948427504949532	8.603766163475699
>50	0.0937741373681566	3.438472158192564
>100	0.0958644135657437	10.936295643853896
>500	0.01255367181570081	4.508623271074185
>1k	0.005380145063871776	4.1105990233098675
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3856	0.3257166653573803	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2622	0.2214805748358535	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2017	0.17037617064985375	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2013	0.1700382902915992	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1778	0.15018781924414473	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1713	0.1446972634225084	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1694	0.14309233172079933	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1671	0.1411495196608357	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1661	0.14030481876519935	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1634	0.13802412634698116	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1567	0.13236463034621754	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1495	0.12628278389763575	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1444	0.12197480932989034	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1420	0.11994752718036308	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1355	0.11445697135872675	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1342	0.11335886019439947	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1340	0.11318992001527219	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1253	0.10584102222323587	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1199	0.10127963738679951	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1195	0.10094175702854498	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1188	0.10035046640159953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.534102686909079E-4
2	0.0	0.0	0.0	0.0	2.534102686909079E-4
3	0.0	0.0	0.0	8.447008956363596E-5	2.534102686909079E-4
4	0.0	0.0	0.0	8.447008956363596E-5	2.534102686909079E-4
5	0.0	0.0	0.0	8.447008956363596E-5	2.534102686909079E-4
6	8.447008956363596E-5	0.0	0.0	8.447008956363596E-5	2.534102686909079E-4
7	8.447008956363596E-5	0.0	0.0	8.447008956363596E-5	2.534102686909079E-4
8	8.447008956363596E-5	0.0	0.0	8.447008956363596E-5	2.534102686909079E-4
9	8.447008956363596E-5	0.0	0.0	8.447008956363596E-5	2.534102686909079E-4
10	8.447008956363596E-5	0.0	0.0	8.447008956363596E-5	4.2235044781817983E-4
11	8.447008956363596E-5	0.0	0.0	8.447008956363596E-5	4.2235044781817983E-4
12	8.447008956363596E-5	0.0	0.0	8.447008956363596E-5	6.757607165090877E-4
13	8.447008956363596E-5	0.0	0.0	8.447008956363596E-5	7.602308060727236E-4
14	8.447008956363596E-5	0.0	0.0	8.447008956363596E-5	7.602308060727236E-4
15	8.447008956363596E-5	0.0	0.0	8.447008956363596E-5	8.447008956363597E-4
16	8.447008956363596E-5	0.0	0.0	1.6894017912727193E-4	8.447008956363597E-4
17	8.447008956363596E-5	0.0	0.0	2.534102686909079E-4	8.447008956363597E-4
18	8.447008956363596E-5	0.0	0.0	2.534102686909079E-4	9.291709851999956E-4
19	8.447008956363596E-5	0.0	0.0	2.534102686909079E-4	9.291709851999956E-4
20	8.447008956363596E-5	0.0	0.0	3.3788035825454385E-4	0.0013515214330181754
21	8.447008956363596E-5	0.0	0.0	3.3788035825454385E-4	0.0015204616121454473
22	8.447008956363596E-5	0.0	0.0	4.2235044781817983E-4	0.0015204616121454473
23	8.447008956363596E-5	0.0	0.0	0.0011825812538909036	0.0015204616121454473
24	8.447008956363596E-5	0.0	0.0	0.001942812059963627	0.0015204616121454473
25	8.447008956363596E-5	0.0	0.0	0.002703042866036351	0.0015204616121454473
26	8.447008956363596E-5	0.0	0.0	0.004139034388618163	0.0018583419703999912
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTTCG	85	1.05610525E-8	20.706621	9
ACACCGT	50	7.210764E-4	19.194197	6
GCCGGTC	460	0.0	18.433104	8
CGGTCCA	430	0.0	18.233208	10
CGCCGGT	440	0.0	18.180162	7
GTATTAG	275	0.0	18.055315	1
CCGGTCC	490	0.0	17.633282	9
GGACCGT	55	0.0013674455	17.44927	6
GTATTAC	75	3.204809E-5	17.084599	1
GATATAC	125	2.8139766E-9	16.657484	1
CTTGCGC	495	0.0	16.47917	3
GGTCCAA	530	0.0	16.30247	11
GTCCTAT	535	0.0	16.166502	1
CTATTAG	70	3.6670725E-4	16.016811	1
ATACCGT	260	0.0	15.995163	6
CTAGCGG	495	0.0	15.837608	29
TAGCGGC	485	0.0	15.834947	30
ACCGTCG	275	0.0	15.70766	8
CGTCGTA	265	0.0	15.698673	10
TACCGTC	265	0.0	15.696684	7
>>END_MODULE