FastQCFastQC Report
Thu 2 Feb 2017
SRR4062920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183851
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT47440.400726104889889No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34520.29159074917367134No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20900.17654248718799917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20250.17105193136636282No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19990.1688557090377083No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17900.15120146031890838No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17880.1510325201397811No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17700.14951205852763566No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17140.14478173351207205No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16810.14199422055647207No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16360.13819306652610844No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16060.13565896383919937No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15280.12907029685323576No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15230.12864794640541757No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15110.12763430533065395No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14680.12400209147941761No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14150.11952517673254488No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13780.11639978341869035No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG12630.10668572311887223No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA12590.10634784276061769No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT12370.10448950079021768No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT12060.10187092801374498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA200.003754507331.99835214
TCGCGTA2050.017.952579
TCCTATA1352.3646862E-1117.7783642
TCTAGCG4100.017.56007228
TACCGTC2750.017.4573357
CTAGCGG4150.017.34850529
ACCGTCG2800.017.1441488
TTTTCGG4400.017.09002929
TAGCGGC4150.016.96298230
GTTTTCG4450.016.89800628
GATATAC957.6766446E-716.8455071
GTCCTAT4500.016.358861
CGCGTAA2250.016.35540410
GCGTAAC2250.016.35540411
GTCGCGT2350.016.3416568
ATACCGT3050.016.2648936
TAGGACG805.6154408E-516.001884
TATACTC805.6154408E-516.001883
CTAAGAC1304.793037E-916.0018793
CTATTCC4300.016.0018794
Produced by FastQC (version 0.11.5)