FastQCFastQC Report
Thu 2 Feb 2017
SRR4062919_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062919_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900319
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT83870.4413469528010823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT57150.3007389811921051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT35150.1849689446877077No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA24750.13024129106744708No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23820.125347375887943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT703.7064223E-415.9952126
ACCGTCG3450.015.30339058
GCACCGT859.461464E-515.0543166
CGACTGG4150.014.65045726
CGACTTT4050.014.6171424
ATACCGT3800.014.3115056
CTTTCGT4250.014.30461127
TTCGTTC4150.014.26491829
AACCGCG2400.013.9991257
TTTCGTT4350.013.97650628
CCGTCGT3550.013.9727969
TAATACT4150.013.8753644
CGATAAC1851.8189894E-1013.83879410
TGGCGAT4100.013.658401530
GCGATGC4100.013.658401532
CGTCGTA3650.013.5899810
GTCCTAG2600.013.54902651
CCGATAA1902.7830538E-1013.4746159
TACCGTC4050.013.4312597
CCGACTT4450.013.3032423
Produced by FastQC (version 0.11.5)