##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062919_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1900319
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210999311168283	32.0	32.0	32.0	32.0	32.0
2	30.81447483290963	32.0	32.0	32.0	32.0	32.0
3	30.8899800507178	32.0	32.0	32.0	32.0	32.0
4	30.942352836550075	32.0	32.0	32.0	32.0	32.0
5	30.865195264584525	32.0	32.0	32.0	32.0	32.0
6	34.529797365600196	36.0	36.0	36.0	32.0	36.0
7	34.44807108701223	36.0	36.0	36.0	32.0	36.0
8	34.42549908725851	36.0	36.0	36.0	32.0	36.0
9	34.56259975298884	36.0	36.0	36.0	32.0	36.0
10	34.2829372331698	36.0	36.0	36.0	32.0	36.0
11	34.58814862136305	36.0	36.0	36.0	32.0	36.0
12	34.39177001335039	36.0	36.0	36.0	32.0	36.0
13	34.48322939464374	36.0	36.0	36.0	32.0	36.0
14	34.38154015194291	36.0	36.0	36.0	32.0	36.0
15	34.334937449975506	36.0	36.0	36.0	32.0	36.0
16	34.34603821779396	36.0	36.0	36.0	32.0	36.0
17	34.281486950348864	36.0	36.0	36.0	32.0	36.0
18	34.30308016706669	36.0	36.0	36.0	32.0	36.0
19	34.29463526913113	36.0	36.0	36.0	32.0	36.0
20	34.25752939374915	36.0	36.0	36.0	32.0	36.0
21	34.24097585721134	36.0	36.0	36.0	32.0	36.0
22	34.21869538745863	36.0	36.0	36.0	32.0	36.0
23	34.17219161624969	36.0	36.0	36.0	32.0	36.0
24	34.17467488353271	36.0	36.0	36.0	32.0	36.0
25	34.14420473615219	36.0	36.0	36.0	32.0	36.0
26	34.10113459898049	36.0	36.0	36.0	32.0	36.0
27	34.106097449954454	36.0	36.0	36.0	32.0	36.0
28	34.09212821636788	36.0	36.0	36.0	32.0	36.0
29	34.05437823860099	36.0	36.0	36.0	32.0	36.0
30	34.020981214206664	36.0	36.0	36.0	32.0	36.0
31	34.03879664414238	36.0	36.0	36.0	32.0	36.0
32	33.997451480514584	36.0	36.0	36.0	32.0	36.0
33	33.95007259307516	36.0	36.0	36.0	32.0	36.0
34	33.938522953251535	36.0	36.0	36.0	32.0	36.0
35	33.8979055621714	36.0	36.0	36.0	32.0	36.0
36	33.85162017534951	36.0	36.0	36.0	32.0	36.0
37	33.84589218967973	36.0	36.0	36.0	32.0	36.0
38	33.24999907910198	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	4.0
8	4.0
9	13.0
10	14.0
11	17.0
12	12.0
13	12.0
14	317.0
15	774.0
16	852.0
17	1013.0
18	1160.0
19	1586.0
20	1926.0
21	2731.0
22	4027.0
23	5887.0
24	8726.0
25	12770.0
26	18605.0
27	26312.0
28	36115.0
29	49122.0
30	65991.0
31	88528.0
32	120479.0
33	180679.0
34	399923.0
35	872717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.12725672902391	17.800030032640738	11.807833250348406	25.264879987986944
2	15.847921285870514	20.450793928266606	39.000077399910595	24.701207385952284
3	18.118476617925065	25.556868945017285	29.258065942208994	27.06658849484866
4	11.97685175927261	16.345800658220867	37.39008164085811	34.287265941648414
5	13.71999574807111	37.57221175026864	34.12846508524381	14.579327416416445
6	31.9956954504911	37.030597558825775	17.915503482317405	13.058203508365718
7	28.80893029952371	31.61651450784264	21.82425353243696	17.750301660196694
8	27.43240448537108	33.56561110260373	20.121653771408994	18.880330640616194
9	27.84175853320851	14.352650697701586	19.074982989626434	38.73060777946347
10	15.40473766053344	27.373342700250745	32.77452829940457	24.44739133981124
11	36.05725487100551	21.955716935706672	23.161384536448672	18.82564365683914
12	24.16723544638727	24.710422272238585	29.80481163962748	21.31753064174666
13	29.301050934093464	20.53842477779228	25.619150073893802	24.54137421422045
14	23.25524361067342	20.407531726910165	26.6504992837302	29.686725378686212
15	24.438605300917665	27.67048702457388	23.819126212876785	24.07178146163167
16	24.36567511766542	26.678932745852695	25.001223625605302	23.954168510876585
17	22.94205206860651	26.537598730023714	26.215999132575877	24.30435006879389
18	23.7127768465868	25.701467480969058	27.484916021776712	23.10083965066743
19	24.6074800095571	25.809432689281632	26.879975244163905	22.703112056997366
20	25.017853393249865	24.89565566553819	26.593740971340367	23.492749969871575
21	26.22748768275881	24.89177352485183	25.680382194968693	23.20035659742067
22	24.863012400982647	25.056836960022565	26.187366196323282	23.892784442671505
23	23.449500823627854	25.22311631309437	26.664596632861965	24.662786230415815
24	23.706978900826144	26.0493140360637	26.594937861409342	23.648769201700816
25	23.97343115943936	25.397664173644845	26.60842013294947	24.02048453396633
26	23.6485746768285	25.998042022822016	26.925344225019998	23.428039075329487
27	24.196454791273496	25.989847074587058	26.06331526801681	23.75038286612264
28	23.439036853702447	25.735966264408432	26.847960913361774	23.977035968527343
29	23.47077061046024	26.084013870914603	26.747929441274128	23.69728607735103
30	23.6314819794669	26.00322378804041	26.909105069914524	23.456189162578166
31	23.899261183422173	25.957148743738163	25.953991183038006	24.18959888980166
32	23.37938184478231	26.30064678423509	26.0069888483662	24.312982522616398
33	23.343543107349984	25.875619691180653	26.428894713021144	24.35194248844822
34	23.85606846063566	25.919676643492085	26.698963722389173	23.525291173483083
35	24.51961462853654	25.87078319039361	26.422366492367043	23.187235688702806
36	23.62599913579721	26.373921917540738	26.219239091018657	23.780839855643396
37	24.25229761683598	26.35437427405939	26.012974963173395	23.38035314593123
38	23.411968956935564	26.450755947140276	26.382763068499663	23.754512027424497
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	849.0
1	782.5
2	716.0
3	716.0
4	1537.5
5	2359.0
6	2359.0
7	2719.0
8	3079.0
9	2956.5
10	2834.0
11	2834.0
12	3594.5
13	4355.0
14	5594.0
15	6833.0
16	6833.0
17	10356.5
18	13880.0
19	13880.0
20	16674.5
21	19469.0
22	20825.5
23	22182.0
24	22182.0
25	24994.5
26	27807.0
27	27807.0
28	34979.5
29	42152.0
30	49925.5
31	57699.0
32	57699.0
33	68199.0
34	78699.0
35	78699.0
36	86413.5
37	94128.0
38	105012.0
39	115896.0
40	115896.0
41	123357.0
42	130818.0
43	143378.5
44	155939.0
45	155939.0
46	160185.5
47	164432.0
48	164432.0
49	170222.5
50	176013.0
51	171324.0
52	166635.0
53	166635.0
54	157404.5
55	148174.0
56	148174.0
57	139984.5
58	131795.0
59	117232.5
60	102670.0
61	102670.0
62	94620.5
63	86571.0
64	70799.5
65	55028.0
66	55028.0
67	45128.5
68	35229.0
69	35229.0
70	28359.5
71	21490.0
72	16625.5
73	11761.0
74	11761.0
75	8936.0
76	6111.0
77	6111.0
78	5696.0
79	5281.0
80	4105.0
81	2929.0
82	2929.0
83	2947.0
84	2965.0
85	2965.0
86	2003.5
87	1042.0
88	865.0
89	688.0
90	688.0
91	529.0
92	370.0
93	333.5
94	297.0
95	297.0
96	260.0
97	223.0
98	223.0
99	572.0
100	921.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12545262137567428
2	0.05746403630127363
3	0.01215585383296173
4	0.0023680234739535838
5	2.6311371932817595E-4
6	2.1049097546254076E-4
7	1.0524548773127038E-4
8	4.2098195092508153E-4
9	4.2098195092508153E-4
10	7.367184141188926E-4
11	0.0023154007300879484
12	0.0027363826810130297
13	0.015523709440362382
14	0.010366680541530133
15	0.02615350370122069
16	0.011997985601364823
17	0.02320663004474512
18	0.006525220239338763
19	0.008472261762367265
20	0.006314729263876223
21	0.007209315909592021
22	0.007735543348248374
23	0.009945698590605052
24	0.015365841208765475
25	0.01836533760910668
26	0.02099647480238844
27	0.00931422566421743
28	0.006840956702532574
29	0.011997985601364823
30	0.0036835920705944633
31	0.006209483776144952
32	0.007840788835979643
33	0.009419471151948699
34	0.013103063222543163
35	0.017575996451122154
36	0.015892068647421827
37	0.010419303285395769
38	0.006314729263876223
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1900319.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.54876606870079
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.44338240475423	42.463355667019314
2	14.112894952545151	15.679078005421577
3	4.566971845153768	7.610689520063685
4	1.8599310795642816	4.132675057704897
5	0.9162761030122221	2.544900345028336
6	0.52432886728193	1.747549295502648
7	0.33032554987481	1.2844423687557542
8	0.22587146307041683	1.0037504850955021
9	0.15355572120187333	0.767684775199815
>10	0.7295346812630732	7.132257153739609
>50	0.0636086257488152	2.4853667449057215
>100	0.06276421986874345	7.293286297024096
>500	0.00751175176758496	2.8010339284924464
>1k	0.0028525639623740353	2.309079599704028
>5k	1.901709308249357E-4	0.7448507563425257
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	8387	0.4413469528010823	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5715	0.3007389811921051	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3515	0.1849689446877077	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2475	0.13024129106744708	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2382	0.125347375887943	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.0524548773127038E-4	0.0	0.0	0.0
6	0.0	1.0524548773127038E-4	0.0	0.0	0.0
7	0.0	1.0524548773127038E-4	0.0	0.0	5.262274386563519E-5
8	0.0	1.0524548773127038E-4	0.0	0.0	5.262274386563519E-5
9	0.0	1.0524548773127038E-4	0.0	0.0	5.262274386563519E-5
10	0.0	1.0524548773127038E-4	0.0	0.0	5.262274386563519E-5
11	0.0	1.0524548773127038E-4	0.0	0.0	5.262274386563519E-5
12	0.0	1.0524548773127038E-4	0.0	0.0	2.6311371932817595E-4
13	0.0	1.5786823159690558E-4	0.0	0.0	2.6311371932817595E-4
14	0.0	1.5786823159690558E-4	0.0	0.0	2.6311371932817595E-4
15	0.0	1.5786823159690558E-4	0.0	0.0	2.6311371932817595E-4
16	0.0	1.5786823159690558E-4	0.0	0.0	2.6311371932817595E-4
17	0.0	1.5786823159690558E-4	5.262274386563519E-5	5.262274386563519E-5	2.6311371932817595E-4
18	0.0	1.5786823159690558E-4	5.262274386563519E-5	2.6311371932817595E-4	2.6311371932817595E-4
19	0.0	1.5786823159690558E-4	5.262274386563519E-5	3.683592070594463E-4	2.6311371932817595E-4
20	0.0	1.5786823159690558E-4	5.262274386563519E-5	3.683592070594463E-4	2.6311371932817595E-4
21	0.0	1.5786823159690558E-4	5.262274386563519E-5	5.788501825219871E-4	2.6311371932817595E-4
22	0.0	1.5786823159690558E-4	5.262274386563519E-5	7.367184141188926E-4	2.6311371932817595E-4
23	0.0	1.5786823159690558E-4	5.262274386563519E-5	0.001631305059834691	2.6311371932817595E-4
24	0.0	1.5786823159690558E-4	5.262274386563519E-5	0.002789005424878665	2.6311371932817595E-4
25	0.0	1.5786823159690558E-4	5.262274386563519E-5	0.003315232863535017	2.6311371932817595E-4
26	0.0	1.5786823159690558E-4	5.262274386563519E-5	0.004525555972444626	3.1573646319381116E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCGT	70	3.7064223E-4	15.995212	6
ACCGTCG	345	0.0	15.3033905	8
GCACCGT	85	9.461464E-5	15.054316	6
CGACTGG	415	0.0	14.650457	26
CGACTTT	405	0.0	14.61714	24
ATACCGT	380	0.0	14.311505	6
CTTTCGT	425	0.0	14.304611	27
TTCGTTC	415	0.0	14.264918	29
AACCGCG	240	0.0	13.999125	7
TTTCGTT	435	0.0	13.976506	28
CCGTCGT	355	0.0	13.972796	9
TAATACT	415	0.0	13.875364	4
CGATAAC	185	1.8189894E-10	13.838794	10
TGGCGAT	410	0.0	13.6584015	30
GCGATGC	410	0.0	13.6584015	32
CGTCGTA	365	0.0	13.58998	10
GTCCTAG	260	0.0	13.5490265	1
CCGATAA	190	2.7830538E-10	13.474615	9
TACCGTC	405	0.0	13.431259	7
CCGACTT	445	0.0	13.30324	23
>>END_MODULE