FastQCFastQC Report
Thu 2 Feb 2017
SRR4062919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900319
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT100430.5284902166425742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT75780.3987751530137835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT50370.26506076085120445No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29520.15534233989135507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG450.00884865217.7790645
ATCGTTT3800.016.8415529
CATCGTT3800.016.8415528
CGGCATC3900.015.99947325
TATACCG859.431531E-515.0599145
GTACCGG1308.3220584E-814.76874529
CGAACGA1308.3220584E-814.76874516
GCATTCG5350.014.65372422
GACGGCC5600.014.57133211
TCGTTTA4300.014.51115130
CAAGACG4200.014.4772374
CGTTTAT4450.014.3815531
GTATAAT3050.014.1668231
TAGGACG2150.014.1405584
CGCATCG4300.014.139070513
ATTCGTA5850.013.94825924
GGACGGC5750.013.91331810
CTAGCGG3700.013.83738229
GCATCGT5000.013.75954727
CGTGCGC1053.6845733E-513.71455610
Produced by FastQC (version 0.11.5)