FastQCFastQC Report
Thu 2 Feb 2017
SRR4062918_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062918_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991669
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33050.3332765267443068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22170.2235624991806742No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14280.1439996611772678No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13970.14087361811249519No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13670.13784841514658622No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13670.13784841514658622No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13340.13452069188408633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12750.128571126051132No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12310.12413416170113213No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12070.12171399932840496No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11990.12090727853749587No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11900.11999971764772319No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11850.119495517153405No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11790.11889047656022322No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11070.11162998944204165No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT10600.10689050479545091No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT10500.10588210380681458No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10370.10457118252158734No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10010.10094093896249655No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA9990.10073925876476929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGGT350.002067458422.8550197
GTATTAT400.004463251720.0153031
CGTTCAA601.1370329E-418.66869514
CAAGACG3700.017.29224
GTGCTAT652.0877967E-417.2439541
TCTTACA854.974987E-616.9438742
CATCGTT3050.016.78532428
AAGACGG4100.016.7746985
TATACTA1051.193257E-716.756125
TCTAGAT2950.016.2737772
ATCGTTT3150.016.25327729
CTAGCGG3250.016.24546429
TCTAGCG3250.016.24464428
GTCTAGA703.674295E-416.0122431
GACGGAC3950.015.7967
TAGCGGC3350.015.76052330
GCGATTT3350.015.759727527
TTAGGAC2850.015.716253
ATACCGT2750.015.7044626
ACCGTCG2350.015.6581198
Produced by FastQC (version 0.11.5)