##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062918_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	991669
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.236058604231857	32.0	32.0	32.0	32.0	32.0
2	30.900860065203208	32.0	32.0	32.0	32.0	32.0
3	30.950879779442534	32.0	32.0	32.0	32.0	32.0
4	31.00437141828574	32.0	32.0	32.0	32.0	32.0
5	30.953196076513432	32.0	32.0	32.0	32.0	32.0
6	34.60585336437864	36.0	36.0	36.0	32.0	36.0
7	34.52782329587796	36.0	36.0	36.0	32.0	36.0
8	34.496522529190685	36.0	36.0	36.0	32.0	36.0
9	34.61178276219182	36.0	36.0	36.0	32.0	36.0
10	34.36831341909448	36.0	36.0	36.0	32.0	36.0
11	34.609944447189534	36.0	36.0	36.0	32.0	36.0
12	34.43503830411155	36.0	36.0	36.0	32.0	36.0
13	34.51377929530922	36.0	36.0	36.0	32.0	36.0
14	34.42744605306811	36.0	36.0	36.0	32.0	36.0
15	34.38251069661349	36.0	36.0	36.0	32.0	36.0
16	34.39246865637627	36.0	36.0	36.0	32.0	36.0
17	34.32330646616966	36.0	36.0	36.0	32.0	36.0
18	34.350273125407774	36.0	36.0	36.0	32.0	36.0
19	34.32631654312074	36.0	36.0	36.0	32.0	36.0
20	34.30324332009975	36.0	36.0	36.0	32.0	36.0
21	34.28272034317902	36.0	36.0	36.0	32.0	36.0
22	34.24450698771465	36.0	36.0	36.0	32.0	36.0
23	34.205615986785915	36.0	36.0	36.0	32.0	36.0
24	34.18314881275909	36.0	36.0	36.0	32.0	36.0
25	34.160603991856156	36.0	36.0	36.0	32.0	36.0
26	34.1282897821753	36.0	36.0	36.0	32.0	36.0
27	34.12652306364321	36.0	36.0	36.0	32.0	36.0
28	34.108360753436884	36.0	36.0	36.0	32.0	36.0
29	34.07289730746852	36.0	36.0	36.0	32.0	36.0
30	34.03485134656826	36.0	36.0	36.0	32.0	36.0
31	34.05348357163529	36.0	36.0	36.0	32.0	36.0
32	34.003007051748114	36.0	36.0	36.0	32.0	36.0
33	33.961768493317834	36.0	36.0	36.0	32.0	36.0
34	33.96846024227842	36.0	36.0	36.0	32.0	36.0
35	33.92623345087927	36.0	36.0	36.0	32.0	36.0
36	33.87350315478249	36.0	36.0	36.0	32.0	36.0
37	33.8626588105507	36.0	36.0	36.0	32.0	36.0
38	33.27011734762305	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	2.0
9	8.0
10	6.0
11	10.0
12	3.0
13	3.0
14	240.0
15	407.0
16	471.0
17	550.0
18	693.0
19	785.0
20	1049.0
21	1419.0
22	2077.0
23	2988.0
24	4293.0
25	6477.0
26	9303.0
27	13120.0
28	18314.0
29	25122.0
30	33487.0
31	45074.0
32	61420.0
33	91409.0
34	206351.0
35	466584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.46978171758577	17.38687074692567	11.329180380833147	25.814167154655415
2	16.6193962912178	19.760126807700885	37.6598086785786	25.960668222502715
3	18.738427279951914	24.060869986163162	28.712347058562504	28.488355675322424
4	12.403290736886264	15.353074527647353	35.81056278942189	36.4330719460445
5	14.583322829135184	36.664828011822514	33.28244259413694	15.469406564905356
6	34.1866112178899	35.456292743726216	16.84528863548816	13.511807402895734
7	30.16853405723079	30.697944576264863	20.818438410397018	18.315082956107332
8	28.392306660629668	32.77978506811286	19.537282322724554	19.29062594853292
9	27.85164344814025	13.955720933984765	18.658317072801804	39.534318545073184
10	16.442917488940274	26.27127617199829	31.131303943585557	26.154502395475877
11	37.659481350312404	20.78632423471137	22.36328873435439	19.19090568062183
12	25.161777622703426	23.83397587226803	28.58409158978217	22.420154915246375
13	29.94624415039535	19.177827981281265	25.141802485073423	25.73412538324996
14	23.726917918049153	20.15157780086126	24.747017356313723	31.374486924775862
15	25.129661719583712	27.482636122110982	22.39602350553647	24.99167865276884
16	25.61794602597345	25.422192101061867	24.23052369469649	24.729338178268186
17	23.71983858099746	25.891894454323282	25.61170075958917	24.776566205090084
18	25.061516492470787	24.117591296525628	26.958947321713104	23.86194488929048
19	25.803406649925876	24.844340907028105	25.306124507104748	24.046127935941268
20	25.824750429355298	23.97592584935705	25.37547788782406	24.823845833463594
21	27.20111737477436	23.870977501235366	24.246528373049888	24.68137675094039
22	25.879953932387107	24.391832881868037	24.982401929837657	24.745811255907203
23	24.084202076107815	24.04487039201587	25.861187393287594	26.009740138588715
24	24.926173657308606	25.101057370733304	25.293086546258458	24.679682425699635
25	24.954941598057875	24.398305178766122	25.407492352426797	25.239260870749202
26	24.267477314011202	25.586830073698575	26.107773197602352	24.037919414687874
27	25.20928740927349	24.874367044884977	24.99659633776028	24.919749208081253
28	24.15937297992862	24.754162670644035	25.921656778716656	25.16480757071069
29	24.19739863668957	25.01701868878945	25.750914975135064	25.034667699385913
30	24.16842050064691	25.102935852534745	26.179438645391407	24.549205001426934
31	25.09527406357117	24.78870285080974	24.69310103840914	25.42292204720995
32	24.397256530162135	25.085746038437218	24.785823993965252	25.731173437435395
33	24.02861707019936	24.737387754443432	25.70685454266743	25.527140632689775
34	25.050325552875112	24.81150609554392	25.794316742373514	24.34385160920746
35	25.705865682043505	24.805221918421644	25.666935279718977	23.821977119815877
36	24.241333159860375	25.528058746819244	25.126347819333965	25.104260273986416
37	25.30451585055679	25.276277917734834	24.921589311539027	24.497616920169346
38	24.485533627131634	25.374996218271296	25.340305160294875	24.799164994302195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	634.0
1	497.0
2	360.0
3	360.0
4	680.5
5	1001.0
6	1001.0
7	1118.5
8	1236.0
9	1187.5
10	1139.0
11	1139.0
12	1459.0
13	1779.0
14	2229.0
15	2679.0
16	2679.0
17	3898.0
18	5117.0
19	5117.0
20	6146.0
21	7175.0
22	7463.0
23	7751.0
24	7751.0
25	8908.0
26	10065.0
27	10065.0
28	13218.5
29	16372.0
30	20328.5
31	24285.0
32	24285.0
33	30851.0
34	37417.0
35	37417.0
36	40365.0
37	43313.0
38	49289.5
39	55266.0
40	55266.0
41	56854.5
42	58443.0
43	65930.5
44	73418.0
45	73418.0
46	76100.5
47	78783.0
48	78783.0
49	83537.5
50	88292.0
51	89284.0
52	90276.0
53	90276.0
54	86715.5
55	83155.0
56	83155.0
57	81554.5
58	79954.0
59	71632.5
60	63311.0
61	63311.0
62	60626.5
63	57942.0
64	47830.5
65	37719.0
66	37719.0
67	31291.0
68	24863.0
69	24863.0
70	19942.5
71	15022.0
72	11775.0
73	8528.0
74	8528.0
75	6442.5
76	4357.0
77	4357.0
78	4329.0
79	4301.0
80	3386.5
81	2472.0
82	2472.0
83	2566.0
84	2660.0
85	2660.0
86	1763.0
87	866.0
88	744.0
89	622.0
90	622.0
91	460.5
92	299.0
93	240.5
94	182.0
95	182.0
96	181.5
97	181.0
98	181.0
99	307.5
100	434.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12191567952613222
2	0.056672135561361706
3	0.01139493117159052
4	0.0037310836579544184
5	2.0168019772726583E-4
6	3.025202965908988E-4
7	0.0
8	6.050405931817976E-4
9	4.0336039545453166E-4
10	8.067207909090633E-4
11	0.0017142816806817597
12	0.003025202965908988
13	0.015025174730681306
14	0.00998316978749966
15	0.0245041440238628
16	0.011899131665908684
17	0.02137810095909018
18	0.006554606426136141
19	0.007966367810227002
20	0.0066554465249997734
21	0.005949565832954342
22	0.008369728205681532
23	0.010285690084090558
24	0.015428535126135836
25	0.01794953759772666
26	0.01996633957499932
27	0.009075608897726963
28	0.00584872573409071
29	0.011294091072726888
30	0.0032268831636362533
31	0.005647045536363444
32	0.007865527711363368
33	0.009378129194317861
34	0.012605012357954117
35	0.015731055422726736
36	0.01613441581818127
37	0.009579809392045129
38	0.005949565832954342
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	991669.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.21016849674272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.63332500697729	42.90512810073879
2	11.685009706570721	12.435226707454044
3	3.4180449038501557	5.4562423578989865
4	1.377985971173286	2.9329146284911274
5	0.7463918277147076	1.9857817458645677
6	0.4435727002688046	1.4161546873114945
7	0.30896896709483995	1.1508203559566692
8	0.20104094993668337	0.855793825670091
9	0.14628906520178386	0.700565922775611
>10	0.8208821709546936	8.133963923817769
>50	0.09763855333624946	3.633967986913421
>100	0.10638621880981465	11.85442322491881
>500	0.010847968583254566	3.892300678795782
>1k	0.0036159895277515217	2.6467158533928234
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3305	0.3332765267443068	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2217	0.2235624991806742	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1428	0.1439996611772678	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1397	0.14087361811249519	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1367	0.13784841514658622	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1367	0.13784841514658622	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1334	0.13452069188408633	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1275	0.128571126051132	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1231	0.12413416170113213	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1207	0.12171399932840496	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1199	0.12090727853749587	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1190	0.11999971764772319	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1185	0.119495517153405	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1179	0.11889047656022322	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1107	0.11162998944204165	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1060	0.10689050479545091	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1050	0.10588210380681458	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1037	0.10457118252158734	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1001	0.10094093896249655	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	999	0.10073925876476929	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0084009886363293E-4
2	0.0	0.0	0.0	0.0	1.0084009886363293E-4
3	0.0	0.0	0.0	0.0	1.0084009886363293E-4
4	0.0	0.0	0.0	0.0	1.0084009886363293E-4
5	0.0	0.0	0.0	0.0	1.0084009886363293E-4
6	0.0	0.0	0.0	0.0	1.0084009886363293E-4
7	0.0	0.0	0.0	1.0084009886363293E-4	1.0084009886363293E-4
8	0.0	0.0	0.0	1.0084009886363293E-4	1.0084009886363293E-4
9	0.0	0.0	0.0	1.0084009886363293E-4	1.0084009886363293E-4
10	0.0	0.0	0.0	1.0084009886363293E-4	1.0084009886363293E-4
11	0.0	0.0	0.0	1.0084009886363293E-4	1.0084009886363293E-4
12	0.0	0.0	0.0	1.0084009886363293E-4	1.0084009886363293E-4
13	0.0	0.0	0.0	1.0084009886363293E-4	1.0084009886363293E-4
14	0.0	0.0	0.0	1.0084009886363293E-4	1.0084009886363293E-4
15	0.0	0.0	0.0	1.0084009886363293E-4	1.0084009886363293E-4
16	0.0	0.0	0.0	1.0084009886363293E-4	1.0084009886363293E-4
17	0.0	0.0	0.0	2.0168019772726586E-4	1.0084009886363293E-4
18	0.0	0.0	0.0	2.0168019772726586E-4	1.0084009886363293E-4
19	0.0	0.0	0.0	2.0168019772726586E-4	1.0084009886363293E-4
20	0.0	0.0	0.0	4.033603954545317E-4	1.0084009886363293E-4
21	0.0	0.0	0.0	8.067207909090634E-4	1.0084009886363293E-4
22	0.0	0.0	0.0	0.001310921285227228	1.0084009886363293E-4
23	0.0	0.0	0.0	0.0024201623727271903	1.0084009886363293E-4
24	0.0	0.0	0.0	0.0031260430647726207	1.0084009886363293E-4
25	0.0	0.0	0.0	0.0035294034602271524	1.0084009886363293E-4
26	0.0	0.0	0.0	0.004235284152272583	2.0168019772726586E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTGGT	35	0.0020674584	22.855019	7
GTATTAT	40	0.0044632517	20.015303	1
CGTTCAA	60	1.1370329E-4	18.668695	14
CAAGACG	370	0.0	17.2922	4
GTGCTAT	65	2.0877967E-4	17.243954	1
TCTTACA	85	4.974987E-6	16.943874	2
CATCGTT	305	0.0	16.785324	28
AAGACGG	410	0.0	16.774698	5
TATACTA	105	1.193257E-7	16.75612	5
TCTAGAT	295	0.0	16.273777	2
ATCGTTT	315	0.0	16.253277	29
CTAGCGG	325	0.0	16.245464	29
TCTAGCG	325	0.0	16.244644	28
GTCTAGA	70	3.674295E-4	16.012243	1
GACGGAC	395	0.0	15.796	7
TAGCGGC	335	0.0	15.760523	30
GCGATTT	335	0.0	15.7597275	27
TTAGGAC	285	0.0	15.71625	3
ATACCGT	275	0.0	15.704462	6
ACCGTCG	235	0.0	15.658119	8
>>END_MODULE