FastQCFastQC Report
Thu 2 Feb 2017
SRR4062918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991669
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38770.3909570632943049No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29380.29626821046135354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17090.17233572895794866No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15320.15448703145908566No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14540.1466215037477223No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13970.14087361811249519No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13840.139562696827268No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13820.1393610166295407No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13310.1342181715874954No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12610.1271593646670411No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12420.1252434027886321No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12270.12373080130567761No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12050.12151231913067767No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11960.12060475824090498No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11630.1172770349784051No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11380.11475603250681427No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11240.11334427112272341No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11130.11223503003522345No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11100.11193250973863256No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11050.11142830924431438No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG10100.10184849985226925No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT10060.10144513945681473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGGC350.002064013922.8615743
CGTATCA400.004480641320.001861
AGACCGT801.2638156E-720.001866
CATCGTT2100.019.04650928
CGTTTAT2250.018.4878131
ATCGTTT2200.018.1807629
AACGAAT1159.422365E-1018.08590131
ACATAAC450.008844702517.779431
ACCGTCG2650.016.9080858
CGGCATC2600.016.61441625
TACCGTC2700.016.5949737
TGTAGGA5050.016.477612
TCGTTTA2550.016.31277530
CTAGCGG3050.016.26134729
CTTATAT703.690307E-416.0031033
CCTTATA805.612721E-516.0022952
AAGACGG3100.016.0014885
GTATAGA805.6151424E-516.0014881
GTCTTAG1203.104651E-816.0014881
ATACCGT2900.016.0014886
Produced by FastQC (version 0.11.5)