FastQCFastQC Report
Thu 2 Feb 2017
SRR4062917_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062917_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358400
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT82202.2935267857142856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT55331.5438058035714286No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA33570.9366629464285714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT32060.89453125No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25930.7234933035714286No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA18400.5133928571428571No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA18000.5022321428571429No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA13120.3660714285714286No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA12950.361328125No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11990.3345424107142857No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9540.2661830357142857No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT9390.26199776785714285No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9150.2553013392857143No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA9130.25474330357142855No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT8170.22795758928571427No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA7290.20340401785714285No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC7210.201171875No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA7110.19838169642857145No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.19838169642857145No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG6320.1763392857142857No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA6250.17438616071428573No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG6100.17020089285714285No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC5910.16489955357142858No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG5750.16043526785714285No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA5720.1595982142857143No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC5610.15652901785714285No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG5480.1529017857142857No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA5480.1529017857142857No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG5450.15206473214285715No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT5410.15094866071428573No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC5340.14899553571428573No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5300.14787946428571427No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG5200.14508928571428573No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5080.14174107142857142No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC5020.1400669642857143No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG5000.13950892857142858No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG4820.13448660714285715No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4650.12974330357142858No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC4590.12806919642857142No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA4580.12779017857142858No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC4320.12053571428571427No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA4250.11858258928571427No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA4240.11830357142857144No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG4210.11746651785714285No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA4210.11746651785714285No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA4090.11411830357142858No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC4090.11411830357142858No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT4080.11383928571428571No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA4030.11244419642857144No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.11160714285714285No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC3990.11132812499999999No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA3990.11132812499999999No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT3950.11021205357142858No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG3920.10937500000000001No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA3800.10602678571428571No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT3760.10491071428571429No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT3750.10463169642857142No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT3710.103515625No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA3690.10295758928571429No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC3690.10295758928571429No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG3680.10267857142857142No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG3630.10128348214285714No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC3620.10100446428571429No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT3590.10016741071428573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG350.00206530222.8542525
TCTAGCG1800.021.3365928
GTCCTAT1450.020.9833581
TAGCGGC1850.020.75992430
GTTCAAA1650.020.3809641
GTGTCTG400.00445557220.017021
TACAGGA1450.019.8678722
AGTAAAC1052.764864E-1019.8070226
CTAGCGG1950.019.69531229
GCCCCTT902.0296284E-819.5530836
TCCTATT1500.019.205612
CACCTCT2000.019.19757324
CTCTAGC2000.019.19757327
CCTCTAG2000.019.19757326
ATACAGG1600.019.0161671
CTATTCC1600.018.9975974
GTGGGTA852.3842404E-718.8237749
ACCTCTA2050.018.7293425
TCCATTA1550.018.5782958
CCTATTC1550.018.5782953
Produced by FastQC (version 0.11.5)