##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062917_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 358400 Sequences flagged as poor quality 0 Sequence length 38 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.65990513392857 32.0 32.0 32.0 32.0 32.0 2 30.456015625 32.0 32.0 32.0 32.0 32.0 3 30.633018973214284 32.0 32.0 32.0 32.0 32.0 4 30.534213169642857 32.0 32.0 32.0 32.0 32.0 5 30.676169084821428 32.0 32.0 32.0 32.0 32.0 6 34.21006138392857 36.0 36.0 36.0 32.0 36.0 7 33.90537388392857 36.0 36.0 36.0 32.0 36.0 8 33.82604073660714 36.0 36.0 36.0 32.0 36.0 9 33.76690290178571 36.0 36.0 36.0 32.0 36.0 10 33.72532366071429 36.0 36.0 36.0 32.0 36.0 11 34.09055245535714 36.0 36.0 36.0 32.0 36.0 12 33.90345424107143 36.0 36.0 36.0 32.0 36.0 13 34.010281808035714 36.0 36.0 36.0 32.0 36.0 14 33.898892299107146 36.0 36.0 36.0 32.0 36.0 15 33.95139787946429 36.0 36.0 36.0 32.0 36.0 16 33.908099888392854 36.0 36.0 36.0 32.0 36.0 17 33.774829799107145 36.0 36.0 36.0 32.0 36.0 18 33.89630022321428 36.0 36.0 36.0 32.0 36.0 19 33.78399832589286 36.0 36.0 36.0 32.0 36.0 20 33.60689732142857 36.0 36.0 36.0 27.0 36.0 21 33.52276506696428 36.0 36.0 36.0 21.0 36.0 22 33.59146763392857 36.0 36.0 36.0 27.0 36.0 23 33.654235491071425 36.0 36.0 36.0 27.0 36.0 24 33.685212053571426 36.0 36.0 36.0 27.0 36.0 25 33.67883370535714 36.0 36.0 36.0 27.0 36.0 26 33.648046875 36.0 36.0 36.0 27.0 36.0 27 33.70317801339286 36.0 36.0 36.0 27.0 36.0 28 33.70449497767857 36.0 36.0 36.0 27.0 36.0 29 33.63526506696429 36.0 36.0 36.0 27.0 36.0 30 33.55766462053571 36.0 36.0 36.0 27.0 36.0 31 33.58921316964286 36.0 36.0 36.0 27.0 36.0 32 33.50342912946429 36.0 36.0 36.0 21.0 36.0 33 33.30693917410714 36.0 36.0 36.0 21.0 36.0 34 33.214464285714286 36.0 36.0 36.0 21.0 36.0 35 33.14763113839286 36.0 36.0 36.0 21.0 36.0 36 33.106294642857144 36.0 36.0 36.0 14.0 36.0 37 33.0865625 36.0 36.0 36.0 14.0 36.0 38 32.31415178571429 36.0 32.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 2.0 10 1.0 11 4.0 12 2.0 13 0.0 14 181.0 15 337.0 16 388.0 17 472.0 18 585.0 19 791.0 20 1010.0 21 1217.0 22 1704.0 23 2218.0 24 2987.0 25 3782.0 26 4935.0 27 6624.0 28 8701.0 29 11281.0 30 15106.0 31 19360.0 32 25444.0 33 35922.0 34 72950.0 35 142395.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.00796176119745 17.077000008380804 12.462530834364639 20.45250739605711 2 16.057106326195044 21.01175570686387 38.69584516301936 24.23529280392173 3 19.37592958976663 29.530886797244086 27.14639312194264 23.946790491046638 4 11.499150350323536 15.324108565417443 33.93325018206779 39.24349090219123 5 13.407403480478461 39.332420012332626 29.24310614705956 18.017070360129352 6 31.020454856179846 35.55088044330481 18.11416884533718 15.314495855178167 7 29.01674107142857 32.75306919642857 19.742745535714285 18.48744419642857 8 31.938147148848074 28.23863905870273 18.388444164185728 21.434769628263474 9 30.714929450137973 12.354945186789026 19.386214805286844 37.543910557786155 10 19.185149429264197 25.52727840913847 26.6545199876115 28.633052173985828 11 36.584698919900774 22.94213335639926 20.8988456805797 19.57432204312026 12 28.523516149914062 25.587903747851513 25.98467599723208 19.903904105002344 13 31.257813546457115 20.222295396704915 26.150265660579542 22.369625396258428 14 26.365650567579337 20.061724950050785 26.535589512339406 27.037034970030472 15 25.086584858743187 26.704137932959178 26.782838755410683 21.426438452886952 16 23.318515790031782 26.839565910162715 26.07385331469281 23.768064985112694 17 21.141185893410803 26.503576322318317 26.3791097008615 25.97612808340938 18 23.058142033199307 23.47501681171714 31.437492500997543 22.029348654086014 19 23.99713147232485 23.80598930720034 30.75240253144777 21.444476689027045 20 27.072026697843913 20.65064833236137 29.546209201938716 22.731115767855997 21 29.39577764260083 21.088627092064804 27.360775494031444 22.154819771302925 22 26.456176577280466 22.707257861986214 28.546474314256216 22.290091246477104 23 22.48716374595379 23.963612010269003 30.390668601406407 23.1585556423708 24 23.020510673922143 25.543184037951722 29.881958978652158 21.554346309473978 25 23.69153231995401 24.61607583880069 29.182422231462386 22.509969609782914 26 22.36553858518657 26.54903066108161 30.189737593382766 20.895693160349058 27 23.019504980885678 25.7089042051511 29.66626670759271 21.60532410637051 28 20.997706358020213 26.05069451032697 30.714712234431417 22.2368868972214 29 20.50562973504667 26.270422613815526 30.984052572098282 22.239895079039528 30 20.69812215742627 26.993777728173217 30.44002343815397 21.86807667624655 31 22.099447513812155 27.680953178190748 27.198225347396622 23.02137396060048 32 20.97262062884377 28.015302534796245 27.516937707186944 23.495139129173037 33 20.503669391969193 27.485838658369843 28.535312665680724 23.475179283980243 34 22.416814564288025 27.26171739834131 28.540892297045396 21.780575740325265 35 23.23757750975325 27.519520458109852 28.12201751399501 21.120884518141885 36 20.8375075694365 29.822044989660917 27.153138420924854 22.18730901997773 37 22.394236108591983 30.3580945896548 26.633163024703588 20.61450627704963 38 20.833810016769956 30.628747778190128 26.9220573750136 21.61538483002631 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 190.0 1 272.0 2 354.0 3 354.0 4 810.5 5 1267.0 6 1267.0 7 1794.5 8 2322.0 9 2516.0 10 2710.0 11 2710.0 12 3417.5 13 4125.0 14 4674.0 15 5223.0 16 5223.0 17 7318.5 18 9414.0 19 9414.0 20 10203.5 21 10993.0 22 8428.0 23 5863.0 24 5863.0 25 4821.0 26 3779.0 27 3779.0 28 5412.5 29 7046.0 30 9293.0 31 11540.0 32 11540.0 33 11891.5 34 12243.0 35 12243.0 36 12529.0 37 12815.0 38 14465.0 39 16115.0 40 16115.0 41 17194.5 42 18274.0 43 21871.0 44 25468.0 45 25468.0 46 26774.5 47 28081.0 48 28081.0 49 28059.5 50 28038.0 51 26867.5 52 25697.0 53 25697.0 54 23896.0 55 22095.0 56 22095.0 57 22433.5 58 22772.0 59 20504.0 60 18236.0 61 18236.0 62 18826.5 63 19417.0 64 16642.5 65 13868.0 66 13868.0 67 12286.5 68 10705.0 69 10705.0 70 8961.0 71 7217.0 72 5757.0 73 4297.0 74 4297.0 75 3338.5 76 2380.0 77 2380.0 78 2285.5 79 2191.0 80 1701.0 81 1211.0 82 1211.0 83 1205.0 84 1199.0 85 1199.0 86 805.5 87 412.0 88 345.5 89 279.0 90 279.0 91 215.0 92 151.0 93 131.5 94 112.0 95 112.0 96 99.5 97 87.0 98 87.0 99 150.5 100 214.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12248883928571427 2 0.05329241071428571 3 0.011997767857142858 4 0.004743303571428571 5 2.7901785714285713E-4 6 2.7901785714285713E-4 7 0.0 8 2.7901785714285713E-4 9 2.7901785714285713E-4 10 8.370535714285715E-4 11 0.001953125 12 0.002232142857142857 13 0.013392857142857144 14 0.010044642857142856 15 0.022600446428571428 16 0.011439732142857144 17 0.019810267857142856 18 0.005301339285714286 19 0.0078125 20 0.005301339285714286 21 0.006138392857142858 22 0.008370535714285714 23 0.011160714285714286 24 0.013950892857142856 25 0.017020089285714288 26 0.017578125 27 0.008370535714285714 28 0.005022321428571428 29 0.009765625 30 0.0027901785714285715 31 0.005580357142857143 32 0.0078125 33 0.008370535714285714 34 0.012276785714285716 35 0.016183035714285712 36 0.014229910714285714 37 0.00864955357142857 38 0.005859375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 358400.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.19005766098328 #Duplication Level Percentage of deduplicated Percentage of total 1 72.69596674142554 25.581752613516873 2 13.58984682803144 9.564549869647143 3 5.438187510574763 5.741103962050952 4 2.596394498143419 3.6546908840130663 5 1.3928608928726192 2.4507427566958064 6 0.8903729472294181 1.8799365211669707 7 0.5665065811350531 1.3954779478828319 8 0.3932001562310118 1.1069388936061553 9 0.3014205470612508 0.9546305788161485 >10 1.6154750629728856 11.206311177991482 >50 0.24762170067424866 6.294553154012047 >100 0.244457206096942 17.16622919192998 >500 0.019778091108166827 4.552345562777753 >1k 0.006328989154613385 4.6219447794298105 >5k 0.0015822472886533462 3.8287921064629673 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 8220 2.2935267857142856 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 5533 1.5438058035714286 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA 3357 0.9366629464285714 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 3206 0.89453125 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 2593 0.7234933035714286 No Hit GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 1840 0.5133928571428571 No Hit GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA 1800 0.5022321428571429 No Hit GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA 1312 0.3660714285714286 No Hit TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA 1295 0.361328125 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 1199 0.3345424107142857 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC 954 0.2661830357142857 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 939 0.26199776785714285 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 915 0.2553013392857143 No Hit ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA 913 0.25474330357142855 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT 817 0.22795758928571427 No Hit GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA 729 0.20340401785714285 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC 721 0.201171875 No Hit GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA 711 0.19838169642857145 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 711 0.19838169642857145 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG 632 0.1763392857142857 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA 625 0.17438616071428573 No Hit GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG 610 0.17020089285714285 No Hit GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC 591 0.16489955357142858 No Hit ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG 575 0.16043526785714285 No Hit GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA 572 0.1595982142857143 No Hit TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC 561 0.15652901785714285 No Hit GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG 548 0.1529017857142857 No Hit TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA 548 0.1529017857142857 No Hit GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG 545 0.15206473214285715 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT 541 0.15094866071428573 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC 534 0.14899553571428573 No Hit CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA 530 0.14787946428571427 No Hit GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG 520 0.14508928571428573 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 508 0.14174107142857142 No Hit GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC 502 0.1400669642857143 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 500 0.13950892857142858 No Hit GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG 482 0.13448660714285715 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 465 0.12974330357142858 No Hit GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC 459 0.12806919642857142 No Hit TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA 458 0.12779017857142858 No Hit CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC 432 0.12053571428571427 No Hit GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA 425 0.11858258928571427 No Hit GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA 424 0.11830357142857144 No Hit ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG 421 0.11746651785714285 No Hit GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA 421 0.11746651785714285 No Hit ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA 409 0.11411830357142858 No Hit ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC 409 0.11411830357142858 No Hit CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT 408 0.11383928571428571 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 403 0.11244419642857144 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 400 0.11160714285714285 No Hit GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC 399 0.11132812499999999 No Hit GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA 399 0.11132812499999999 No Hit CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT 395 0.11021205357142858 No Hit GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG 392 0.10937500000000001 No Hit ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA 380 0.10602678571428571 No Hit GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT 376 0.10491071428571429 No Hit GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT 375 0.10463169642857142 No Hit GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT 371 0.103515625 No Hit ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA 369 0.10295758928571429 No Hit CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC 369 0.10295758928571429 No Hit GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG 368 0.10267857142857142 No Hit GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG 363 0.10128348214285714 No Hit TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC 362 0.10100446428571429 No Hit ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT 359 0.10016741071428573 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 2.7901785714285713E-4 0.0 17 0.0 0.0 0.0 8.370535714285714E-4 0.0 18 0.0 0.0 0.0 8.370535714285714E-4 0.0 19 0.0 0.0 0.0 8.370535714285714E-4 0.0 20 0.0 0.0 0.0 8.370535714285714E-4 0.0 21 0.0 0.0 0.0 0.0016741071428571428 0.0 22 0.0 2.7901785714285713E-4 0.0 0.0030691964285714285 0.0 23 0.0 2.7901785714285713E-4 0.0 0.007533482142857143 0.0 24 0.0 2.7901785714285713E-4 0.0 0.012276785714285714 0.0 25 0.0 2.7901785714285713E-4 0.0 0.016462053571428572 0.0 26 0.0 2.7901785714285713E-4 0.0 0.01953125 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACAG 35 0.002065302 22.854252 5 TCTAGCG 180 0.0 21.33659 28 GTCCTAT 145 0.0 20.983358 1 TAGCGGC 185 0.0 20.759924 30 GTTCAAA 165 0.0 20.380964 1 GTGTCTG 40 0.004455572 20.01702 1 TACAGGA 145 0.0 19.867872 2 AGTAAAC 105 2.764864E-10 19.80702 26 CTAGCGG 195 0.0 19.695312 29 GCCCCTT 90 2.0296284E-8 19.553083 6 TCCTATT 150 0.0 19.20561 2 CACCTCT 200 0.0 19.197573 24 CTCTAGC 200 0.0 19.197573 27 CCTCTAG 200 0.0 19.197573 26 ATACAGG 160 0.0 19.016167 1 CTATTCC 160 0.0 18.997597 4 GTGGGTA 85 2.3842404E-7 18.823774 9 ACCTCTA 205 0.0 18.72934 25 TCCATTA 155 0.0 18.578295 8 CCTATTC 155 0.0 18.578295 3 >>END_MODULE