FastQCFastQC Report
Thu 2 Feb 2017
SRR4062917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358400
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT103482.8872767857142856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT75052.094029017857143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT48211.3451450892857142No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32520.9073660714285715No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA26860.7494419642857143No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA15280.4263392857142857No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA13680.38169642857142855No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA13500.37667410714285715No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.33984375No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11990.3345424107142857No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11910.33231026785714285No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11870.33119419642857145No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA11260.31417410714285715No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA11240.3136160714285714No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9920.27678571428571425No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT7980.22265624999999997No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA7760.21651785714285715No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC7280.203125No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC7000.1953125No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6490.1810825892857143No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC6430.17940848214285715No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG6300.17578125No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA6280.17522321428571427No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG6200.17299107142857142No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC5960.16629464285714285No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA5920.16517857142857142No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT5690.1587611607142857No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA5690.1587611607142857No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5580.1556919642857143No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG5410.15094866071428573No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG5370.14983258928571427No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5280.14732142857142858No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG5130.14313616071428573No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5120.14285714285714285No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA5110.142578125No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG5010.13978794642857142No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG4790.13364955357142858No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA4780.1333705357142857No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC4750.13253348214285715No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA4750.13253348214285715No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT4720.13169642857142858No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA4640.1294642857142857No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG4620.12890625No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT4610.12862723214285715No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA4530.12639508928571427No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC4470.12472098214285714No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG4450.12416294642857142No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4420.12332589285714286No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC4390.12248883928571427No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG4330.12081473214285714No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT4300.11997767857142858No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA4290.11969866071428571No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC4160.11607142857142858No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4070.11356026785714285No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA4070.11356026785714285No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA4060.11328124999999999No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT4060.11328124999999999No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT4010.11188616071428571No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG4000.11160714285714285No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA3920.10937500000000001No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC3870.10797991071428571No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT3810.10630580357142856No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT3800.10602678571428571No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC3770.10518973214285715No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTC3740.10435267857142856No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA3660.10212053571428573No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC3660.10212053571428573No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3630.10128348214285714No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT3600.10044642857142856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC1800.022.2246468
CGGACCA1800.022.2215449
TCTAGCG1300.022.1531728
AAGACGG1900.021.0549285
AGACGGA1900.021.0549286
GACGGAC1900.021.0549287
GGACCAG1900.021.05198910
TAGCGGC1400.020.570830
CAAGACG1950.020.5150574
TGTCCAC555.820326E-520.36301410
AGGACCT951.70985E-920.212735
TAGGACA801.2579767E-720.002184
AAGGAGA400.00447474120.002186
CAAAATG400.004478356319.9993921
TGTACAA400.004478356319.9993932
CTGATCT400.004478356319.999399
TGTAGGA1800.019.5576882
CCAGAGC2050.019.511613
CATTTGC2050.019.511625
ATTTGCC2050.019.511626
Produced by FastQC (version 0.11.5)