##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062917_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	358400
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.538353794642855	32.0	32.0	32.0	32.0	32.0
2	31.247837611607142	32.0	32.0	32.0	32.0	32.0
3	31.359676339285713	32.0	32.0	32.0	32.0	32.0
4	31.50748325892857	32.0	32.0	32.0	32.0	32.0
5	31.352430245535714	32.0	32.0	32.0	32.0	32.0
6	34.84626674107143	36.0	36.0	36.0	32.0	36.0
7	34.885320870535715	36.0	36.0	36.0	32.0	36.0
8	34.87296875	36.0	36.0	36.0	32.0	36.0
9	34.91802176339286	36.0	36.0	36.0	32.0	36.0
10	34.76951171875	36.0	36.0	36.0	32.0	36.0
11	34.976010044642855	36.0	36.0	36.0	36.0	36.0
12	34.87277622767857	36.0	36.0	36.0	32.0	36.0
13	34.884832589285715	36.0	36.0	36.0	32.0	36.0
14	34.809188058035716	36.0	36.0	36.0	32.0	36.0
15	34.7993359375	36.0	36.0	36.0	32.0	36.0
16	34.79151227678572	36.0	36.0	36.0	32.0	36.0
17	34.72416573660714	36.0	36.0	36.0	32.0	36.0
18	34.707943638392855	36.0	36.0	36.0	32.0	36.0
19	34.66299107142857	36.0	36.0	36.0	32.0	36.0
20	34.61728236607143	36.0	36.0	36.0	32.0	36.0
21	34.58821149553572	36.0	36.0	36.0	32.0	36.0
22	34.62150390625	36.0	36.0	36.0	32.0	36.0
23	34.58558035714286	36.0	36.0	36.0	32.0	36.0
24	34.56722098214286	36.0	36.0	36.0	32.0	36.0
25	34.54962332589286	36.0	36.0	36.0	32.0	36.0
26	34.480047433035715	36.0	36.0	36.0	32.0	36.0
27	34.493133370535716	36.0	36.0	36.0	32.0	36.0
28	34.44708984375	36.0	36.0	36.0	32.0	36.0
29	34.37619140625	36.0	36.0	36.0	32.0	36.0
30	34.3250390625	36.0	36.0	36.0	32.0	36.0
31	34.28395089285714	36.0	36.0	36.0	32.0	36.0
32	34.197287946428574	36.0	36.0	36.0	32.0	36.0
33	34.07560546875	36.0	36.0	36.0	32.0	36.0
34	33.94619419642857	36.0	36.0	36.0	32.0	36.0
35	33.845948660714285	36.0	36.0	36.0	32.0	36.0
36	33.786902901785716	36.0	36.0	36.0	32.0	36.0
37	33.725337611607145	36.0	36.0	36.0	32.0	36.0
38	33.319921875	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	7.0
22	27.0
23	111.0
24	291.0
25	738.0
26	1592.0
27	3116.0
28	5367.0
29	8228.0
30	12451.0
31	17708.0
32	24767.0
33	37324.0
34	81521.0
35	165147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.116487380185006	18.442370209144308	13.26357205642292	21.177570354247763
2	15.456194196428571	21.900390625	37.606863839285715	25.03655133928571
3	18.713169642857146	29.28404017857143	28.025669642857142	23.977120535714285
4	11.02405705388981	15.867276045067216	34.032835004659624	39.07583189638335
5	12.779575892857142	39.158482142857146	29.647321428571427	18.414620535714285
6	31.26171875	35.75390625	17.2265625	15.757812500000002
7	28.014587094272024	32.85165416198148	20.23275734586313	18.901001397883366
8	31.332172593138242	28.50035715082926	18.6335074443626	21.533962811669905
9	29.606687649277884	12.172704747873835	19.865677805308156	38.35492979754012
10	18.95952789519943	25.60359380013672	27.02241939759204	28.414458907071804
11	36.501199843741276	22.513254087839723	21.03688821920866	19.948657849210335
12	27.62152690584211	25.833012461007037	26.537815501202573	20.00764513194828
13	31.528547791707005	19.89570283399228	26.469660908652088	22.10608846564862
14	25.859654017857142	20.034877232142854	27.132533482142858	26.972935267857146
15	25.220424107142858	26.474888392857142	26.82421875	21.48046875
16	23.292689732142858	25.863281249999996	26.880859375	23.963169642857142
17	21.100167410714285	25.728515624999996	27.59263392857143	25.578683035714285
18	22.99358258928571	21.650390625	32.999720982142854	22.356305803571427
19	23.883370535714285	22.82142857142857	32.247767857142854	21.047433035714285
20	26.079241071428573	19.798828125	31.723214285714285	22.398716517857142
21	28.126395089285715	20.584263392857142	29.378348214285715	21.91099330357143
22	25.479352678571427	21.944475446428573	30.552176339285715	22.023995535714285
23	21.74420129520451	22.404917424434778	32.59691014762876	23.253971132731955
24	22.65904017857143	23.897321428571427	31.84263392857143	21.601004464285715
25	23.08900669642857	23.002790178571427	31.445033482142858	22.463169642857142
26	21.81333705357143	25.02232142857143	32.315011160714285	20.849330357142858
27	22.97935267857143	23.885044642857142	31.558593750000004	21.577008928571427
28	20.898995535714285	24.092354910714285	32.76171875	22.24693080357143
29	20.481584821428573	24.194754464285715	32.98046875	22.343191964285715
30	20.74609375	24.844308035714285	32.818917410714285	21.59068080357143
31	22.076729910714285	25.308035714285715	29.69224330357143	22.922991071428573
32	21.060546875	25.773995535714285	29.89425223214286	23.271205357142858
33	20.5390625	25.015345982142854	31.095982142857142	23.349609375
34	22.23828125	25.01729910714286	30.929129464285715	21.815290178571427
35	23.254464285714285	25.224609375	30.618582589285715	20.90234375
36	21.326450892857142	27.278180803571427	29.51646205357143	21.87890625
37	22.698939732142858	27.693359375	29.0234375	20.584263392857142
38	21.097935267857142	27.73967633928571	29.52845982142857	21.63392857142857
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	66.0
1	90.0
2	114.0
3	114.0
4	584.0
5	1054.0
6	1054.0
7	1746.5
8	2439.0
9	2802.5
10	3166.0
11	3166.0
12	3886.0
13	4606.0
14	5231.5
15	5857.0
16	5857.0
17	8620.5
18	11384.0
19	11384.0
20	11964.0
21	12544.0
22	9716.5
23	6889.0
24	6889.0
25	4969.5
26	3050.0
27	3050.0
28	4483.0
29	5916.0
30	7971.5
31	10027.0
32	10027.0
33	10851.0
34	11675.0
35	11675.0
36	11853.0
37	12031.0
38	13883.0
39	15735.0
40	15735.0
41	16737.5
42	17740.0
43	21356.5
44	24973.0
45	24973.0
46	26652.5
47	28332.0
48	28332.0
49	28594.0
50	28856.0
51	27376.0
52	25896.0
53	25896.0
54	24176.0
55	22456.0
56	22456.0
57	22672.0
58	22888.0
59	20485.5
60	18083.0
61	18083.0
62	18882.0
63	19681.0
64	16853.0
65	14025.0
66	14025.0
67	12246.5
68	10468.0
69	10468.0
70	8838.0
71	7208.0
72	5712.0
73	4216.0
74	4216.0
75	3218.5
76	2221.0
77	2221.0
78	2185.0
79	2149.0
80	1637.0
81	1125.0
82	1125.0
83	1052.0
84	979.0
85	979.0
86	632.0
87	285.0
88	235.5
89	186.0
90	186.0
91	116.5
92	47.0
93	34.0
94	21.0
95	21.0
96	14.5
97	8.0
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009765625
2	0.0
3	0.0
4	5.580357142857143E-4
5	0.0
6	0.0
7	2.7901785714285713E-4
8	0.002232142857142857
9	0.002232142857142857
10	0.0013950892857142857
11	0.005580357142857143
12	5.580357142857143E-4
13	2.7901785714285713E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.7901785714285713E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	358400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.99803202715908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.25907592684128	18.923847737893492
2	17.332364377844648	10.052089146702606
3	6.8341753316324345	5.945329054376936
4	3.3428810835942153	3.8774789090019723
5	1.804014546255315	2.615643579488624
6	1.054250804073576	1.8342719148710263
7	0.712780354861171	1.4468459291015834
8	0.526620240840801	1.221676048804142
9	0.39023643350847353	1.0184479737338747
>10	2.055709673995235	11.568967911539342
>50	0.3225423406702348	6.671873095740831
>100	0.32951084803039415	19.352242497069653
>500	0.02190102313192952	3.952259196685173
>1k	0.011946012617416103	6.36529948775897
>5k	9.955010514513419E-4	2.166511231547894
>10k+	9.955010514513419E-4	2.987216285683892
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	10348	2.8872767857142856	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	7505	2.094029017857143	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	4821	1.3451450892857142	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3252	0.9073660714285715	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2686	0.7494419642857143	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1528	0.4263392857142857	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1368	0.38169642857142855	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1350	0.37667410714285715	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1218	0.33984375	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1199	0.3345424107142857	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1191	0.33231026785714285	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1187	0.33119419642857145	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	1126	0.31417410714285715	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	1124	0.3136160714285714	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	992	0.27678571428571425	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	798	0.22265624999999997	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	776	0.21651785714285715	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	728	0.203125	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	700	0.1953125	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	649	0.1810825892857143	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	643	0.17940848214285715	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	630	0.17578125	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	628	0.17522321428571427	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	620	0.17299107142857142	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	596	0.16629464285714285	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	592	0.16517857142857142	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	569	0.1587611607142857	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	569	0.1587611607142857	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	558	0.1556919642857143	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	541	0.15094866071428573	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	537	0.14983258928571427	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	528	0.14732142857142858	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	513	0.14313616071428573	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	512	0.14285714285714285	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	511	0.142578125	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	501	0.13978794642857142	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	479	0.13364955357142858	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	478	0.1333705357142857	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	475	0.13253348214285715	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	475	0.13253348214285715	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	472	0.13169642857142858	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	464	0.1294642857142857	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	462	0.12890625	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	461	0.12862723214285715	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	453	0.12639508928571427	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	447	0.12472098214285714	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	445	0.12416294642857142	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	442	0.12332589285714286	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	439	0.12248883928571427	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	433	0.12081473214285714	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	430	0.11997767857142858	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	429	0.11969866071428571	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	416	0.11607142857142858	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	407	0.11356026785714285	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	407	0.11356026785714285	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	406	0.11328124999999999	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	406	0.11328124999999999	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	401	0.11188616071428571	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	400	0.11160714285714285	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	392	0.10937500000000001	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	387	0.10797991071428571	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	381	0.10630580357142856	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	380	0.10602678571428571	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	377	0.10518973214285715	No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTC	374	0.10435267857142856	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	366	0.10212053571428573	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	366	0.10212053571428573	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	363	0.10128348214285714	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	360	0.10044642857142856	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.7901785714285713E-4	0.0
10	0.0	0.0	2.7901785714285713E-4	2.7901785714285713E-4	0.0
11	0.0	0.0	2.7901785714285713E-4	2.7901785714285713E-4	0.0
12	0.0	0.0	2.7901785714285713E-4	2.7901785714285713E-4	0.0
13	0.0	0.0	2.7901785714285713E-4	2.7901785714285713E-4	0.0
14	0.0	0.0	2.7901785714285713E-4	5.580357142857143E-4	0.0
15	0.0	0.0	2.7901785714285713E-4	5.580357142857143E-4	0.0
16	0.0	0.0	2.7901785714285713E-4	8.370535714285714E-4	0.0
17	0.0	0.0	2.7901785714285713E-4	0.0013950892857142857	0.0
18	0.0	0.0	2.7901785714285713E-4	0.0013950892857142857	0.0
19	0.0	0.0	2.7901785714285713E-4	0.0013950892857142857	0.0
20	0.0	0.0	2.7901785714285713E-4	0.0013950892857142857	0.0
21	0.0	0.0	2.7901785714285713E-4	0.002232142857142857	0.0
22	0.0	0.0	2.7901785714285713E-4	0.003627232142857143	0.0
23	0.0	0.0	2.7901785714285713E-4	0.008370535714285714	0.0
24	0.0	0.0	2.7901785714285713E-4	0.013671875	0.0
25	0.0	0.0	2.7901785714285713E-4	0.017578125	0.0
26	0.0	0.0	2.7901785714285713E-4	0.020368303571428572	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGACC	180	0.0	22.224646	8
CGGACCA	180	0.0	22.221544	9
TCTAGCG	130	0.0	22.15317	28
AAGACGG	190	0.0	21.054928	5
AGACGGA	190	0.0	21.054928	6
GACGGAC	190	0.0	21.054928	7
GGACCAG	190	0.0	21.051989	10
TAGCGGC	140	0.0	20.5708	30
CAAGACG	195	0.0	20.515057	4
TGTCCAC	55	5.820326E-5	20.363014	10
AGGACCT	95	1.70985E-9	20.21273	5
TAGGACA	80	1.2579767E-7	20.00218	4
AAGGAGA	40	0.004474741	20.00218	6
CAAAATG	40	0.0044783563	19.99939	21
TGTACAA	40	0.0044783563	19.99939	32
CTGATCT	40	0.0044783563	19.99939	9
TGTAGGA	180	0.0	19.557688	2
CCAGAGC	205	0.0	19.5116	13
CATTTGC	205	0.0	19.5116	25
ATTTGCC	205	0.0	19.5116	26
>>END_MODULE