FastQCFastQC Report
Thu 2 Feb 2017
SRR4062916_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062916_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563047
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39160.25053629225480745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26670.1706282664564789No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18370.11752685619818215No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18290.11701503537641543No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16990.10869794702270628No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15750.10076472428532218No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15720.10057279147715967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG3950.019.03732729
TAGGACG1208.367351E-1118.6630484
TCTAGCG4000.017.99938828
TCGCGCA450.00885339517.7771749
ACCGTCG3700.017.728568
TACCGTC4000.016.7988917
TAGCGGC4550.016.17527630
TCGCGTA1900.015.9994559
ATCGTTT2950.015.72827929
ATACCGT4100.015.6067296
TATACTG1755.456968E-1215.5398445
CGCGTAA2000.015.19948310
CATCGTT3200.014.99949128
CCGTGCG756.247743E-414.93282613
CGTACAC650.00416606814.7673113
CCGTCGT4350.014.7121439
CGTTTAT3200.014.49950731
GCGTAAC2100.014.47569811
TCGTTTA3100.014.45112230
CTATACT1553.1432137E-914.4488114
Produced by FastQC (version 0.11.5)