##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062916_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1563047
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24691515994081	32.0	32.0	32.0	32.0	32.0
2	30.839550570136407	32.0	32.0	32.0	32.0	32.0
3	30.88404507350067	32.0	32.0	32.0	32.0	32.0
4	30.957328218537256	32.0	32.0	32.0	32.0	32.0
5	30.85482906144217	32.0	32.0	32.0	32.0	32.0
6	34.5310032263905	36.0	36.0	36.0	32.0	36.0
7	34.444593796603684	36.0	36.0	36.0	32.0	36.0
8	34.42103468417776	36.0	36.0	36.0	32.0	36.0
9	34.5813158529462	36.0	36.0	36.0	32.0	36.0
10	34.281750964622304	36.0	36.0	36.0	32.0	36.0
11	34.56864828760747	36.0	36.0	36.0	32.0	36.0
12	34.37751903813513	36.0	36.0	36.0	32.0	36.0
13	34.47419047539837	36.0	36.0	36.0	32.0	36.0
14	34.36702159308069	36.0	36.0	36.0	32.0	36.0
15	34.313973924008685	36.0	36.0	36.0	32.0	36.0
16	34.321951291291946	36.0	36.0	36.0	32.0	36.0
17	34.246363673005355	36.0	36.0	36.0	32.0	36.0
18	34.270304091943494	36.0	36.0	36.0	32.0	36.0
19	34.27060158779614	36.0	36.0	36.0	32.0	36.0
20	34.250352676535	36.0	36.0	36.0	32.0	36.0
21	34.23580800833244	36.0	36.0	36.0	32.0	36.0
22	34.19575546992509	36.0	36.0	36.0	32.0	36.0
23	34.15433061193937	36.0	36.0	36.0	32.0	36.0
24	34.13736950968205	36.0	36.0	36.0	32.0	36.0
25	34.10335901607565	36.0	36.0	36.0	32.0	36.0
26	34.0698974503006	36.0	36.0	36.0	32.0	36.0
27	34.06984946709856	36.0	36.0	36.0	32.0	36.0
28	34.0486760794781	36.0	36.0	36.0	32.0	36.0
29	34.01961489321818	36.0	36.0	36.0	32.0	36.0
30	33.99048205204322	36.0	36.0	36.0	32.0	36.0
31	34.014650231247046	36.0	36.0	36.0	32.0	36.0
32	33.962017776816694	36.0	36.0	36.0	32.0	36.0
33	33.91470058162039	36.0	36.0	36.0	32.0	36.0
34	33.929654706480356	36.0	36.0	36.0	32.0	36.0
35	33.88499706022915	36.0	36.0	36.0	32.0	36.0
36	33.840250485110175	36.0	36.0	36.0	32.0	36.0
37	33.85026489926407	36.0	36.0	36.0	32.0	36.0
38	33.24171186151152	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	3.0
9	7.0
10	6.0
11	13.0
12	4.0
13	9.0
14	165.0
15	342.0
16	456.0
17	587.0
18	696.0
19	871.0
20	1282.0
21	1929.0
22	2907.0
23	4609.0
24	6890.0
25	10498.0
26	15375.0
27	21960.0
28	30925.0
29	41697.0
30	56414.0
31	75694.0
32	103097.0
33	153903.0
34	338199.0
35	694504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.00749959004632	17.013718074857543	11.284359242405609	25.694423092690528
2	16.77612922471063	19.269635086398605	37.64865463474713	26.30558105414364
3	19.107880857119277	23.357069685570647	28.414646709276504	29.120402748033573
4	12.514771263741995	14.902101790082028	36.022241828380146	36.56088511779583
5	14.629321469265962	36.57394345246664	33.65143160215452	15.14530347611288
6	34.806550303830605	35.348186421094255	16.55489743717699	13.290365837898149
7	30.72929395552018	30.35681611417706	20.433243807283983	18.480646123018772
8	28.546321851876023	32.82573340959488	19.17997085171096	19.44797388681814
9	27.511754330180715	14.04173019133855	18.415255901796563	40.03125957668417
10	15.703117002763845	26.528367796089668	31.626765789743068	26.14174941140342
11	37.93164779510461	21.140585353245186	21.88798366241806	19.03978318923214
12	24.774986308521473	23.675829294133905	28.656072618578442	22.89311177876618
13	29.49076517994542	19.267357078642338	25.252953766712416	25.988923974699823
14	23.61213749860038	19.55621670905514	25.16531503431067	31.666330758033816
15	25.307419798244556	27.03662416713808	22.362422601114492	25.29353343350287
16	25.965949044496327	25.783402958487883	23.57314288310204	24.677505113913746
17	24.145254707026286	25.892660027375396	25.09174725814079	24.870338007457523
18	25.117131942102944	24.818279940703054	26.02558828290299	24.038999834291012
19	25.471928688606404	25.06494138000752	25.594197631655053	23.86893229973102
20	25.72248251575997	24.237241831144242	25.152737176891055	24.887538476204735
21	26.967957076481508	24.100012412298156	24.654215126880562	24.277815384339775
22	25.706803535873142	24.30388137319636	25.143254559321083	24.846060531609414
23	24.392341283170975	24.198346049874434	25.582481565014316	25.82683110194027
24	24.697761380258225	25.047509722696788	25.30633373388669	24.948395163158306
25	24.93458776373657	24.491787396954532	25.30130581316256	25.272319026146338
26	24.622269887301666	25.390719984437748	25.68500776832724	24.302002359933347
27	25.274580435464163	24.864773214411397	25.065997837395148	24.794648512729296
28	24.555814748038035	24.818007209332976	25.656664794168044	24.96951324846095
29	24.451677631621045	25.06459320040592	25.73298405869247	24.75074510928056
30	24.52153217189909	25.21212276313011	25.831952450720085	24.434392614250708
31	25.032918013991274	25.061453357509468	24.77552409700993	25.130104531489327
32	24.673921249726483	25.076105302354623	24.7430846224422	25.506888825476693
33	24.39780003787743	24.704850358555948	25.354397109030696	25.542952494535925
34	25.1294723466007	24.869056013185897	25.45515272426197	24.546318915951428
35	25.756178714827314	24.60288260041911	25.405467662726156	24.23547102202742
36	24.569527609740867	25.39080046428951	24.97827642118276	25.061395504786866
37	25.571943725702468	25.097254107066536	24.993985601273515	24.336816565957477
38	24.57716529437997	24.976087085874713	25.41141408977692	25.0353335299684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	99.0
1	139.0
2	179.0
3	179.0
4	490.0
5	801.0
6	801.0
7	1002.5
8	1204.0
9	1242.5
10	1281.0
11	1281.0
12	1671.0
13	2061.0
14	2745.5
15	3430.0
16	3430.0
17	5043.0
18	6656.0
19	6656.0
20	8173.0
21	9690.0
22	10487.5
23	11285.0
24	11285.0
25	13415.0
26	15545.0
27	15545.0
28	19910.5
29	24276.0
30	29677.0
31	35078.0
32	35078.0
33	43439.5
34	51801.0
35	51801.0
36	58636.5
37	65472.0
38	75368.0
39	85264.0
40	85264.0
41	92118.0
42	98972.0
43	111258.5
44	123545.0
45	123545.0
46	128783.5
47	134022.0
48	134022.0
49	140731.0
50	147440.0
51	148568.0
52	149696.0
53	149696.0
54	142023.0
55	134350.0
56	134350.0
57	128620.5
58	122891.0
59	110495.5
60	98100.0
61	98100.0
62	91415.0
63	84730.0
64	70627.5
65	56525.0
66	56525.0
67	46966.0
68	37407.0
69	37407.0
70	30346.5
71	23286.0
72	18418.5
73	13551.0
74	13551.0
75	10441.0
76	7331.0
77	7331.0
78	6773.5
79	6216.0
80	4955.5
81	3695.0
82	3695.0
83	3489.0
84	3283.0
85	3283.0
86	2266.0
87	1249.0
88	1037.5
89	826.0
90	826.0
91	624.0
92	422.0
93	377.5
94	333.0
95	333.0
96	295.5
97	258.0
98	258.0
99	527.5
100	797.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12123755715599083
2	0.05559653676440951
3	0.011771878900634466
4	0.0029429697251586165
5	5.118208217667158E-4
6	3.198880136041974E-4
7	6.397760272083948E-5
8	3.198880136041974E-4
9	3.838656163250369E-4
10	4.4784321904587645E-4
11	0.0015994400680209874
12	0.001983305684346024
13	0.013563251776817971
14	0.0078052675319424175
15	0.023223869787664733
16	0.009852550819009282
17	0.02111260889787703
18	0.004222521779575407
19	0.00633378266936311
20	0.004350476985017085
21	0.005182185820387999
22	0.006077872258479751
23	0.0078052675319424175
24	0.013371318968655453
25	0.017018042323743304
26	0.01861748239176429
27	0.007485379518338221
28	0.004670364998621283
29	0.012091766914238664
30	0.002367171300671061
31	0.004670364998621283
32	0.005182185820387999
33	0.007101513902013183
34	0.010556304448938516
35	0.014203027804026366
36	0.014650871023072243
37	0.008381065956429973
38	0.005374118628550517
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1563047.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.114122549978106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.90526972025785	43.71585852371647
2	13.368870498557566	15.003648750216925
3	4.21534944380683	7.096219058422756
4	1.711817545281776	3.842285580765771
5	0.831230990373743	2.3321898830585943
6	0.47802822759482955	1.6094480727363056
7	0.3010789063634065	1.1826345054222704
8	0.2091270912442887	0.9387986581281975
9	0.1484614826864523	0.7497707250077132
>10	0.6885405689726026	6.89591482212279
>50	0.06374525347812926	2.502526614771487
>100	0.06656518570991961	8.073533065686837
>500	0.008248905465734169	3.1155044650642214
>1k	0.003666180206992964	2.9416672748796677
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3916	0.25053629225480745	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2667	0.1706282664564789	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1837	0.11752685619818215	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1829	0.11701503537641543	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1699	0.10869794702270628	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1575	0.10076472428532218	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1572	0.10057279147715967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	3.8386561632503696E-4
13	0.0	0.0	0.0	6.397760272083949E-5	3.8386561632503696E-4
14	0.0	0.0	0.0	6.397760272083949E-5	3.8386561632503696E-4
15	0.0	0.0	0.0	6.397760272083949E-5	4.478432190458764E-4
16	0.0	0.0	0.0	6.397760272083949E-5	4.478432190458764E-4
17	0.0	0.0	0.0	1.2795520544167899E-4	4.478432190458764E-4
18	0.0	0.0	0.0	1.9193280816251848E-4	4.478432190458764E-4
19	0.0	0.0	0.0	2.5591041088335797E-4	4.478432190458764E-4
20	0.0	0.0	0.0	3.1988801360419746E-4	5.118208217667159E-4
21	0.0	0.0	0.0	3.1988801360419746E-4	5.118208217667159E-4
22	0.0	0.0	0.0	5.118208217667159E-4	5.757984244875554E-4
23	0.0	0.0	0.0	7.677312326500739E-4	5.757984244875554E-4
24	0.0	0.0	0.0	0.0012795520544167899	5.757984244875554E-4
25	0.0	0.0	0.0	0.0017273952734626662	5.757984244875554E-4
26	0.0	0.0	0.0	0.002111260889787703	7.037536299292344E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	395	0.0	19.037327	29
TAGGACG	120	8.367351E-11	18.663048	4
TCTAGCG	400	0.0	17.999388	28
TCGCGCA	45	0.008853395	17.777174	9
ACCGTCG	370	0.0	17.72856	8
TACCGTC	400	0.0	16.798891	7
TAGCGGC	455	0.0	16.175276	30
TCGCGTA	190	0.0	15.999455	9
ATCGTTT	295	0.0	15.728279	29
ATACCGT	410	0.0	15.606729	6
TATACTG	175	5.456968E-12	15.539844	5
CGCGTAA	200	0.0	15.199483	10
CATCGTT	320	0.0	14.999491	28
CCGTGCG	75	6.247743E-4	14.932826	13
CGTACAC	65	0.004166068	14.767311	3
CCGTCGT	435	0.0	14.712143	9
CGTTTAT	320	0.0	14.499507	31
GCGTAAC	210	0.0	14.475698	11
TCGTTTA	310	0.0	14.451122	30
CTATACT	155	3.1432137E-9	14.448811	4
>>END_MODULE