FastQCFastQC Report
Thu 2 Feb 2017
SRR4062916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563047
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46080.2948087933376284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT32140.20562401514477813No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20760.13281750324846278No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19580.12526814612740372No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18800.12027789311517825No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17310.11074523030977315No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17110.10946567825535637No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16420.10505122366761845No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16390.10485929085945593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG3300.018.90865529
TAGCGGC3350.018.62643830
ATACCGT3100.018.5808126
AAGACGG4900.017.6328125
CAAGACG5200.017.538624
ATCGTTT3500.017.37102929
CATCGTT3600.017.33293528
ACCGTCG3200.017.0006988
CGTCGTA3250.016.73861110
TCTAGCG3750.016.63961828
TACCGTC3500.016.4578177
CGTTTAT3800.016.42067531
CGCATCG3900.016.41040413
CCTAATA1102.021061E-715.9996332
CCGTCGT3350.015.761849
CGCAAGA5100.015.6859142
AGCGGCG4250.015.43493931
CGTGCAA1255.1399184E-815.36013811
GACGGAC5350.015.2529627
GTCGTAG3600.015.11124511
Produced by FastQC (version 0.11.5)