##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062916_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1563047
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21034236334544	32.0	32.0	32.0	32.0	32.0
2	31.301942296041002	32.0	32.0	32.0	32.0	32.0
3	31.383499024661447	32.0	32.0	32.0	32.0	32.0
4	31.48324842439159	32.0	32.0	32.0	32.0	32.0
5	31.40336151120216	32.0	32.0	32.0	32.0	32.0
6	34.93753482780748	36.0	36.0	36.0	36.0	36.0
7	34.95878626810326	36.0	36.0	36.0	36.0	36.0
8	34.89718351399542	36.0	36.0	36.0	36.0	36.0
9	35.00652827458163	36.0	36.0	36.0	36.0	36.0
10	34.86245519168649	36.0	36.0	36.0	32.0	36.0
11	35.03902313877958	36.0	36.0	36.0	36.0	36.0
12	34.941001134322896	36.0	36.0	36.0	32.0	36.0
13	34.99313328389997	36.0	36.0	36.0	36.0	36.0
14	34.936832353729606	36.0	36.0	36.0	32.0	36.0
15	34.90650249160774	36.0	36.0	36.0	32.0	36.0
16	34.923673440401984	36.0	36.0	36.0	32.0	36.0
17	34.882445633432646	36.0	36.0	36.0	32.0	36.0
18	34.87890831177821	36.0	36.0	36.0	32.0	36.0
19	34.87751551936698	36.0	36.0	36.0	32.0	36.0
20	34.86819654175466	36.0	36.0	36.0	32.0	36.0
21	34.86301755481441	36.0	36.0	36.0	32.0	36.0
22	34.835011359223365	36.0	36.0	36.0	32.0	36.0
23	34.78628409766309	36.0	36.0	36.0	32.0	36.0
24	34.76754697715424	36.0	36.0	36.0	32.0	36.0
25	34.74539409243612	36.0	36.0	36.0	32.0	36.0
26	34.68054639431828	36.0	36.0	36.0	32.0	36.0
27	34.66332234411377	36.0	36.0	36.0	32.0	36.0
28	34.63140263856429	36.0	36.0	36.0	32.0	36.0
29	34.6061513185464	36.0	36.0	36.0	32.0	36.0
30	34.58528694274708	36.0	36.0	36.0	32.0	36.0
31	34.57071860283152	36.0	36.0	36.0	32.0	36.0
32	34.54795473200742	36.0	36.0	36.0	32.0	36.0
33	34.50633858098957	36.0	36.0	36.0	32.0	36.0
34	34.49902722055063	36.0	36.0	36.0	32.0	36.0
35	34.46736534474012	36.0	36.0	36.0	32.0	36.0
36	34.43906741128066	36.0	36.0	36.0	32.0	36.0
37	34.435731619074794	36.0	36.0	36.0	32.0	36.0
38	34.010130213614815	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	5.0
21	30.0
22	98.0
23	366.0
24	1011.0
25	2337.0
26	5056.0
27	9991.0
28	17389.0
29	27697.0
30	42331.0
31	61125.0
32	89421.0
33	143212.0
34	354493.0
35	808484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36171241957197	18.142182933134045	12.028757019057029	26.467347628236954
2	15.821169576051874	20.204024836137155	36.901688690984585	27.073116896826388
3	18.109244315749944	23.950079556148985	28.79836626793692	29.142309860164158
4	11.994999497774533	15.69950410833227	36.01801102081005	36.28748537308314
5	14.087749176927614	37.030452078825576	33.34527582681508	15.536522917431734
6	34.81989938856449	35.832475498114896	16.115679479067435	13.231945634253183
7	30.352915735785995	30.616057467646296	20.42013107823492	18.610895718332785
8	28.351871223244817	33.138837439180044	18.958167388028908	19.551123949546227
9	27.316862191102103	13.894967037241774	18.244298057039902	40.54387271461622
10	15.470405032257734	26.758820654162797	31.738296494770434	26.032477818809035
11	38.07417634797163	21.013539537984542	21.749762312713294	19.162521801330538
12	24.57064548406827	23.737463564685317	28.629456368470752	23.062434582775662
13	29.45126164397584	19.264830074726174	25.286365032244856	25.99754324905313
14	23.483810787519506	19.504851741502335	25.05465286712428	31.956684603853887
15	25.235581527618812	27.21671197347233	22.24059801144815	25.30710848746071
16	25.885913859276144	25.894998678862503	23.45930736567742	24.759780096183928
17	24.074899859057343	25.95251454370854	25.053565247878023	24.919020349356096
18	25.056636172808624	24.753638246322726	26.101646335650813	24.088079245217834
19	25.408640942978682	24.955935426126022	25.67817858324158	23.957245047653718
20	25.647149445921976	24.221536524493505	25.171859835308858	24.95945419427567
21	26.862165284962824	24.15040888112058	24.584881059162687	24.40254477475391
22	25.548400717829622	24.355472811083494	25.240028278136585	24.8560981929503
23	24.23386673695686	24.0949071171381	25.683218130775586	25.988008015129456
24	24.64084573272589	25.062074269039897	25.3245743730035	24.97250562523072
25	24.82502092071624	24.471671942696428	25.473371191086112	25.229935945501214
26	24.630225450674228	25.195211660301965	25.71502968240878	24.459533206615028
27	25.375917842206313	24.686684482364825	25.072278974127997	24.86511870130086
28	24.500798760369968	24.70655073072019	25.79084314163298	25.00180736727686
29	24.48243718838909	24.802133269185124	25.885529993659816	24.82989954876597
30	24.559594177270423	25.042625077812758	25.941446418437835	24.456334326478988
31	24.977431900640223	24.84243915889925	24.907504380866346	25.272624559594174
32	24.68454243538422	24.880953675737196	24.906800627236418	25.527703261642166
33	24.39414809663433	24.4741840776381	25.459823025155355	25.671844800572213
34	25.052925471850813	24.718002721607217	25.68841499967691	24.54065680686505
35	25.805685945464212	24.455502617643614	25.450610250363553	24.28820118652862
36	24.489858590304706	25.319136276772227	25.1354565793607	25.055548553562367
37	25.551502929854315	24.9224111623003	25.06495326116233	24.461132646683048
38	24.60303395364043	24.689212005044006	25.531415322547108	25.176338718768456
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	56.0
2	73.0
3	73.0
4	384.0
5	695.0
6	695.0
7	927.0
8	1159.0
9	1221.0
10	1283.0
11	1283.0
12	1651.0
13	2019.0
14	2713.5
15	3408.0
16	3408.0
17	5176.0
18	6944.0
19	6944.0
20	8505.0
21	10066.0
22	10980.5
23	11895.0
24	11895.0
25	13658.0
26	15421.0
27	15421.0
28	19837.0
29	24253.0
30	29414.0
31	34575.0
32	34575.0
33	43516.5
34	52458.0
35	52458.0
36	59274.5
37	66091.0
38	75634.5
39	85178.0
40	85178.0
41	91873.0
42	98568.0
43	111482.5
44	124397.0
45	124397.0
46	128840.0
47	133283.0
48	133283.0
49	140900.5
50	148518.0
51	148305.5
52	148093.0
53	148093.0
54	141312.0
55	134531.0
56	134531.0
57	129154.5
58	123778.0
59	111303.0
60	98828.0
61	98828.0
62	92288.5
63	85749.0
64	71013.5
65	56278.0
66	56278.0
67	46931.0
68	37584.0
69	37584.0
70	30469.5
71	23355.0
72	18342.5
73	13330.0
74	13330.0
75	10165.5
76	7001.0
77	7001.0
78	6620.5
79	6240.0
80	4868.0
81	3496.0
82	3496.0
83	3171.0
84	2846.0
85	2846.0
86	1847.0
87	848.0
88	700.5
89	553.0
90	553.0
91	342.5
92	132.0
93	87.5
94	43.0
95	43.0
96	33.0
97	23.0
98	23.0
99	19.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010172438832613479
2	1.2795520544167896E-4
3	0.0
4	2.559104108833579E-4
5	6.397760272083948E-5
6	2.559104108833579E-4
7	7.037536299292344E-4
8	0.0020472832870668633
9	0.0045424097931796035
10	7.037536299292344E-4
11	0.004414454587737924
12	1.9193280816251845E-4
13	4.4784321904587645E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	6.397760272083948E-5
22	1.2795520544167896E-4
23	1.9193280816251845E-4
24	0.0
25	1.9193280816251845E-4
26	0.0
27	3.838656163250369E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.559104108833579E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1563047.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.948400617549744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.92616715090999	40.96095282812337
2	14.575525031641604	15.726525272362512
3	4.582120354467055	7.41594193681853
4	1.8837377094457028	4.064985464302492
5	0.9132452947816746	2.4634061512487047
6	0.5026211146965234	1.6269363152692475
7	0.3289413408909675	1.2422101466641937
8	0.22288664179363943	0.9619502275026854
9	0.15890575890786032	0.7715440387797321
>10	0.7537791348164886	7.205926375280535
>50	0.06798029367851452	2.5863437114568013
>100	0.07119011720587906	8.376306202103981
>500	0.00895837275293182	3.3745232731696
>1k	0.003941684011290001	3.2224480569176857
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4608	0.2948087933376284	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3214	0.20562401514477813	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2076	0.13281750324846278	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1958	0.12526814612740372	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1880	0.12027789311517825	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1731	0.11074523030977315	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1711	0.10946567825535637	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1642	0.10505122366761845	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1639	0.10485929085945593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5591041088335797E-4
2	0.0	0.0	0.0	0.0	2.5591041088335797E-4
3	0.0	0.0	0.0	0.0	2.5591041088335797E-4
4	0.0	0.0	0.0	0.0	2.5591041088335797E-4
5	0.0	0.0	0.0	0.0	2.5591041088335797E-4
6	0.0	0.0	0.0	0.0	3.1988801360419746E-4
7	0.0	0.0	0.0	0.0	3.8386561632503696E-4
8	0.0	0.0	0.0	0.0	3.8386561632503696E-4
9	0.0	0.0	0.0	0.0	3.8386561632503696E-4
10	0.0	0.0	0.0	0.0	3.8386561632503696E-4
11	0.0	0.0	0.0	0.0	3.8386561632503696E-4
12	0.0	0.0	0.0	0.0	3.8386561632503696E-4
13	0.0	0.0	0.0	0.0	3.8386561632503696E-4
14	0.0	0.0	0.0	0.0	3.8386561632503696E-4
15	0.0	0.0	0.0	0.0	4.478432190458764E-4
16	0.0	0.0	0.0	6.397760272083949E-5	4.478432190458764E-4
17	0.0	0.0	0.0	1.2795520544167899E-4	4.478432190458764E-4
18	0.0	0.0	0.0	1.9193280816251848E-4	5.757984244875554E-4
19	0.0	0.0	0.0	1.9193280816251848E-4	5.757984244875554E-4
20	0.0	0.0	0.0	4.478432190458764E-4	6.397760272083949E-4
21	0.0	0.0	0.0	4.478432190458764E-4	7.037536299292344E-4
22	0.0	0.0	0.0	6.397760272083949E-4	7.677312326500739E-4
23	0.0	0.0	0.0	8.956864380917528E-4	7.677312326500739E-4
24	0.0	0.0	0.0	0.0014075072598584688	7.677312326500739E-4
25	0.0	0.0	0.0	0.0017273952734626662	7.677312326500739E-4
26	0.0	0.0	0.0	0.001983305684346024	8.956864380917528E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	330	0.0	18.908655	29
TAGCGGC	335	0.0	18.626438	30
ATACCGT	310	0.0	18.580812	6
AAGACGG	490	0.0	17.632812	5
CAAGACG	520	0.0	17.53862	4
ATCGTTT	350	0.0	17.371029	29
CATCGTT	360	0.0	17.332935	28
ACCGTCG	320	0.0	17.000698	8
CGTCGTA	325	0.0	16.738611	10
TCTAGCG	375	0.0	16.639618	28
TACCGTC	350	0.0	16.457817	7
CGTTTAT	380	0.0	16.420675	31
CGCATCG	390	0.0	16.410404	13
CCTAATA	110	2.021061E-7	15.999633	2
CCGTCGT	335	0.0	15.76184	9
CGCAAGA	510	0.0	15.685914	2
AGCGGCG	425	0.0	15.434939	31
CGTGCAA	125	5.1399184E-8	15.360138	11
GACGGAC	535	0.0	15.252962	7
GTCGTAG	360	0.0	15.111245	11
>>END_MODULE