FastQCFastQC Report
Thu 2 Feb 2017
SRR4062915_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062915_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534956
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT51470.3353190580055715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35910.23394807408160231No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24280.1581804299276331No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22850.14886420197061023No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22520.14671430321129725No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21320.13889648954106829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20770.13531332494221332No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20460.13329372307740417No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20360.13264223860488508No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19720.12847273798076297No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA19450.12671372990496144No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19440.12664858145770955No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18940.12339115909511411No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18520.12065492431053398No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17860.11635512679190804No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG17750.11563849387213705No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17600.11466126716335842No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT17390.11329314977106836No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT17170.11185988393152638No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.10788582864915998No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT16300.10619196902061036No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA16200.10554048454809127No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC16150.10521474231183173No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16120.10501929697007602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTC1003.270543E-919.197445
TCTAGCG4900.017.63087528
GGACCGT1101.0657459E-817.4522196
CTAGCGG4900.017.30494329
TACCGTC3950.017.0114977
ATACCGT4250.016.9389176
ACCGTCG3800.016.8409568
TAGCGGC5250.016.4560230
CGTCGTA3950.016.20248210
TTATACT2000.015.1979724
GTACACG756.1846944E-414.9527751
AGCGGCG6050.014.808907531
CCGTCGT4350.014.7125999
CGAGCCG5600.014.28567115
TAGGACG901.536455E-414.2203254
AGGCCCG5750.014.19126110
GTATTAG5000.014.09833051
GTCGTAG4550.014.06589111
ACAATAC3450.013.9125483
TTTCGTT5150.013.66852528
Produced by FastQC (version 0.11.5)