##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062915_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1534956
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.222515173073365	32.0	32.0	32.0	32.0	32.0
2	30.849683639140146	32.0	32.0	32.0	32.0	32.0
3	30.906882021373903	32.0	32.0	32.0	32.0	32.0
4	30.96832938533743	32.0	32.0	32.0	32.0	32.0
5	30.892208636599356	32.0	32.0	32.0	32.0	32.0
6	34.54666778721996	36.0	36.0	36.0	32.0	36.0
7	34.46228621537034	36.0	36.0	36.0	32.0	36.0
8	34.450143847771535	36.0	36.0	36.0	32.0	36.0
9	34.57361513945676	36.0	36.0	36.0	32.0	36.0
10	34.30434553172859	36.0	36.0	36.0	32.0	36.0
11	34.57027693301958	36.0	36.0	36.0	32.0	36.0
12	34.391598847133075	36.0	36.0	36.0	32.0	36.0
13	34.483739599050395	36.0	36.0	36.0	32.0	36.0
14	34.372385918554016	36.0	36.0	36.0	32.0	36.0
15	34.323162357748366	36.0	36.0	36.0	32.0	36.0
16	34.33781554650427	36.0	36.0	36.0	32.0	36.0
17	34.266405030502504	36.0	36.0	36.0	32.0	36.0
18	34.28790988145589	36.0	36.0	36.0	32.0	36.0
19	34.27521049463307	36.0	36.0	36.0	32.0	36.0
20	34.24548977299675	36.0	36.0	36.0	32.0	36.0
21	34.22618954549837	36.0	36.0	36.0	32.0	36.0
22	34.205665178676135	36.0	36.0	36.0	32.0	36.0
23	34.154937340223434	36.0	36.0	36.0	32.0	36.0
24	34.13742999799343	36.0	36.0	36.0	32.0	36.0
25	34.1159095114127	36.0	36.0	36.0	32.0	36.0
26	34.06605922254449	36.0	36.0	36.0	32.0	36.0
27	34.06825211927899	36.0	36.0	36.0	32.0	36.0
28	34.05315201217494	36.0	36.0	36.0	32.0	36.0
29	34.0217888981834	36.0	36.0	36.0	32.0	36.0
30	33.98504387096438	36.0	36.0	36.0	32.0	36.0
31	34.00841652790047	36.0	36.0	36.0	32.0	36.0
32	33.9520624695431	36.0	36.0	36.0	32.0	36.0
33	33.909910121202174	36.0	36.0	36.0	32.0	36.0
34	33.909204563518436	36.0	36.0	36.0	32.0	36.0
35	33.86141752597469	36.0	36.0	36.0	32.0	36.0
36	33.82648362558927	36.0	36.0	36.0	32.0	36.0
37	33.818929011645935	36.0	36.0	36.0	32.0	36.0
38	33.20644305113632	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	2.0
9	2.0
10	8.0
11	23.0
12	9.0
13	5.0
14	307.0
15	489.0
16	665.0
17	774.0
18	898.0
19	1143.0
20	1510.0
21	2164.0
22	3336.0
23	4984.0
24	6947.0
25	10414.0
26	15121.0
27	21494.0
28	29764.0
29	40189.0
30	53660.0
31	72097.0
32	98120.0
33	146504.0
34	327527.0
35	696798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.617310162147426	17.274222153516607	11.465184190838107	25.643283493497858
2	16.794543960087424	19.99318190558569	37.13384519014727	26.07842894417962
3	18.46598405650266	24.65501907420861	28.517424151108127	28.361572718180604
4	12.597903997727572	15.473620157897274	35.79895265036269	36.129523194012464
5	14.737715096162555	36.455383324440554	33.15593376474719	15.6509678146497
6	33.89810743020945	35.492947653018014	17.032932668816574	13.57601224795596
7	30.15130736731696	30.671974096960497	21.1187298650449	18.05798867067764
8	28.67366536543162	31.884124063814372	19.589863396379823	19.85234717437418
9	28.10546765570825	13.95422924726083	18.524203331174746	39.416099765856174
10	16.418678617521188	25.925875254812365	30.887460397810223	26.767985729856225
11	37.578277491263414	21.05941401658975	22.15523374447204	19.207074747674806
12	25.31762179601192	23.78673066987725	28.16493074896917	22.730716785141666
13	29.5993656361947	19.129756588300754	25.145005818476562	26.12587195702798
14	24.324162320925495	19.768170671003286	24.710522818073073	31.19714418999815
15	25.48627736674534	26.436546423930906	22.639640614146323	25.43753559517743
16	26.093231205466743	25.317303532428813	23.536559621789827	25.05290564031462
17	24.32782894385314	25.413024694817203	25.019124832046835	25.240021529282824
18	25.52396365335038	24.009715373183823	26.1641410735774	24.302179899888397
19	25.88611264944457	24.79910088933772	25.379483337133923	23.935303124083788
20	26.150986494700884	23.49344801772648	24.912191775377735	25.4433737121949
21	27.82700361462424	23.73996335860356	23.987410057880897	24.445622968891296
22	26.408532374288207	23.76184147088334	24.622636592262488	25.20698956256597
23	24.393693882111794	23.569301295846373	25.554345864387322	26.482658957654508
24	24.97364405332958	24.93513635374437	25.005505754001607	25.085713838924445
25	25.21243357887295	23.95845482711774	25.04986267808681	25.779248915922498
26	24.868035152398953	25.020704520590165	25.60030077755167	24.51095954945921
27	25.80759284850072	24.446472887384278	24.462370320274633	25.283563943840363
28	24.69803808638987	24.15610611650821	25.701244202311713	25.444611594790207
29	24.616724806782393	24.648781142534716	25.637966690079082	25.096527360603805
30	24.54196130858146	24.808556049394298	25.96731803866536	24.682164603358885
31	25.275688945469422	24.706459519363705	24.35731215819848	25.660539376968394
32	24.816434443478748	24.775584184760444	24.347796626954395	26.060184744806413
33	24.43321638341331	24.184852542113372	25.14481918369607	26.237111890777243
34	25.460421295421526	24.432072140162497	25.244105056717856	24.86340150769812
35	26.315861490184634	24.124605143816265	25.1718862397181	24.387647126281
36	24.792294214731715	25.170794067801577	24.7031587699048	25.333752947561905
37	25.925783553280972	24.928998882616046	24.616587450768325	24.528630113334657
38	24.79016315901603	24.627805506999557	25.136834533967733	25.445196800016678
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	940.0
1	725.5
2	511.0
3	511.0
4	1026.5
5	1542.0
6	1542.0
7	1803.5
8	2065.0
9	1932.5
10	1800.0
11	1800.0
12	2251.5
13	2703.0
14	3383.0
15	4063.0
16	4063.0
17	6055.5
18	8048.0
19	8048.0
20	9342.5
21	10637.0
22	10832.5
23	11028.0
24	11028.0
25	12442.5
26	13857.0
27	13857.0
28	17681.5
29	21506.0
30	26457.5
31	31409.0
32	31409.0
33	40184.5
34	48960.0
35	48960.0
36	53960.0
37	58960.0
38	68517.0
39	78074.0
40	78074.0
41	83726.5
42	89379.0
43	103484.5
44	117590.0
45	117590.0
46	121743.0
47	125896.0
48	125896.0
49	134779.0
50	143662.0
51	143906.5
52	144151.0
53	144151.0
54	137755.5
55	131360.0
56	131360.0
57	129508.0
58	127656.0
59	114555.5
60	101455.0
61	101455.0
62	97368.0
63	93281.0
64	76609.5
65	59938.0
66	59938.0
67	50217.5
68	40497.0
69	40497.0
70	32605.0
71	24713.0
72	19163.0
73	13613.0
74	13613.0
75	10194.0
76	6775.0
77	6775.0
78	6736.0
79	6697.0
80	5271.5
81	3846.0
82	3846.0
83	3952.0
84	4058.0
85	4058.0
86	2714.5
87	1371.0
88	1198.5
89	1026.0
90	1026.0
91	754.0
92	482.0
93	415.5
94	349.0
95	349.0
96	315.5
97	282.0
98	282.0
99	529.0
100	776.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12019888517977063
2	0.05231420314328228
3	0.010488900007557221
4	0.002215047206564879
5	1.9544534175572461E-4
6	3.9089068351144923E-4
7	6.514844725190819E-5
8	2.6059378900763276E-4
9	1.3029689450381638E-4
10	1.9544534175572461E-4
11	0.002084750312061062
12	0.0021498987593129705
13	0.012378204977862557
14	0.007817813670228983
15	0.02202017517114497
16	0.009641970193282414
17	0.019674831070076275
18	0.004495242860381665
19	0.00690573540870227
20	0.005016430438396931
21	0.0052118757801526555
22	0.006254250936183188
23	0.008404149695496157
24	0.012899392555877824
25	0.015700775787709873
26	0.017394635416259487
27	0.007622368328473259
28	0.005016430438396931
29	0.010293454665801496
30	0.002736234784580144
31	0.004820985096641207
32	0.005342172674656472
33	0.00723147764496181
34	0.010814642243816761
35	0.014332658395419803
36	0.013876619264656446
37	0.007882962117480892
38	0.004755836649389299
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1534956.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.624555919054224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.45023365650881	39.71508240606801
2	12.967787668112454	13.129769839015616
3	3.914357895333529	5.94487890478511
4	1.6355797878829639	3.312020017270238
5	0.8290754534526155	2.098578832721359
6	0.507805227486281	1.5424448480920372
7	0.3163037488251018	1.1208915773861996
8	0.21727124308329038	0.8799408156057966
9	0.17176826118252822	0.782612274901825
>10	0.7930277245420984	7.195275396077106
>50	0.08178112689060468	2.9671941655763567
>100	0.09314507767541653	10.501760518460289
>500	0.015524115283502787	5.474196496086626
>1k	0.006209646113401114	4.998267681009202
>5k	1.293676273625232E-4	0.3370862269441996
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5147	0.3353190580055715	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3591	0.23394807408160231	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2428	0.1581804299276331	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2285	0.14886420197061023	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2252	0.14671430321129725	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2132	0.13889648954106829	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2077	0.13531332494221332	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2046	0.13329372307740417	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2036	0.13264223860488508	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1972	0.12847273798076297	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1945	0.12671372990496144	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1944	0.12664858145770955	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1894	0.12339115909511411	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1852	0.12065492431053398	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1786	0.11635512679190804	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1775	0.11563849387213705	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1760	0.11466126716335842	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1739	0.11329314977106836	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1717	0.11185988393152638	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1656	0.10788582864915998	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1630	0.10619196902061036	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1620	0.10554048454809127	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1615	0.10521474231183173	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1612	0.10501929697007602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.954453417557246E-4
2	0.0	0.0	0.0	0.0	1.954453417557246E-4
3	0.0	0.0	0.0	0.0	1.954453417557246E-4
4	0.0	0.0	0.0	0.0	1.954453417557246E-4
5	0.0	0.0	0.0	0.0	1.954453417557246E-4
6	0.0	0.0	0.0	0.0	1.954453417557246E-4
7	0.0	0.0	0.0	6.51484472519082E-5	1.954453417557246E-4
8	0.0	0.0	0.0	6.51484472519082E-5	1.954453417557246E-4
9	0.0	0.0	0.0	6.51484472519082E-5	1.954453417557246E-4
10	0.0	0.0	0.0	1.302968945038164E-4	1.954453417557246E-4
11	0.0	0.0	0.0	1.302968945038164E-4	1.954453417557246E-4
12	0.0	0.0	0.0	1.302968945038164E-4	1.954453417557246E-4
13	0.0	0.0	0.0	1.302968945038164E-4	1.954453417557246E-4
14	0.0	0.0	0.0	1.302968945038164E-4	1.954453417557246E-4
15	0.0	0.0	0.0	1.302968945038164E-4	1.954453417557246E-4
16	0.0	0.0	0.0	1.302968945038164E-4	1.954453417557246E-4
17	0.0	0.0	0.0	1.302968945038164E-4	1.954453417557246E-4
18	0.0	0.0	0.0	1.954453417557246E-4	1.954453417557246E-4
19	0.0	0.0	0.0	2.605937890076328E-4	1.954453417557246E-4
20	0.0	0.0	0.0	5.211875780152656E-4	1.954453417557246E-4
21	0.0	0.0	0.0	7.817813670228983E-4	2.605937890076328E-4
22	0.0	0.0	0.0	0.0011726720505343476	2.605937890076328E-4
23	0.0	0.0	0.0	0.0018241565230534295	3.2574223625954097E-4
24	0.0	0.0	0.0	0.0033877192570992264	3.2574223625954097E-4
25	0.0	0.0	0.0	0.004169500624122125	3.2574223625954097E-4
26	0.0	0.0	0.0	0.00508157888564884	3.2574223625954097E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTC	100	3.270543E-9	19.19744	5
TCTAGCG	490	0.0	17.630875	28
GGACCGT	110	1.0657459E-8	17.452219	6
CTAGCGG	490	0.0	17.304943	29
TACCGTC	395	0.0	17.011497	7
ATACCGT	425	0.0	16.938917	6
ACCGTCG	380	0.0	16.840956	8
TAGCGGC	525	0.0	16.45602	30
CGTCGTA	395	0.0	16.202482	10
TTATACT	200	0.0	15.197972	4
GTACACG	75	6.1846944E-4	14.952775	1
AGCGGCG	605	0.0	14.8089075	31
CCGTCGT	435	0.0	14.712599	9
CGAGCCG	560	0.0	14.285671	15
TAGGACG	90	1.536455E-4	14.220325	4
AGGCCCG	575	0.0	14.191261	10
GTATTAG	500	0.0	14.0983305	1
GTCGTAG	455	0.0	14.065891	11
ACAATAC	345	0.0	13.912548	3
TTTCGTT	515	0.0	13.668525	28
>>END_MODULE