FastQCFastQC Report
Thu 2 Feb 2017
SRR4062915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534956
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT62310.40593997482664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT44900.29251652816106777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT27150.17687803428893076No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24660.16065607092320563No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23970.15616082806282394No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22540.14684460010580108No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21200.13811470817404536No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21140.13772381749053395No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20860.13589966096748052No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20290.13218619947412172No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA20070.13075293363457977No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20000.13029689450381637No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19850.12931966779503776No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19490.12697432369396908No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA18850.12280482306984694No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18630.12137155723030497No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC18300.119221658470992No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17960.11700661126442713No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG17670.11511730629412179No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT17470.11381433734908362No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16890.11003572740847295No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT16840.1097099851722134No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG16180.10541018765358746No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT15970.10404207026129739No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15850.10326028889427451No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15780.10280424976351113No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15720.10241335907999968No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15450.10065435100419817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT805.61461E-516.0027374
CATCGTT4400.015.63547128
GTATAAT1652.910383E-1115.5157841
TAATACC1551.891749E-1015.486524
ATCGTTT4350.015.44739529
TCCAATA4500.015.2895132
TAGCGTA3850.014.9635987
GCGACGC756.248746E-414.93248232
ACCGTCG3550.014.8757848
GTTATTC3400.014.5902563
TAAACGC4500.014.57694728
CGCTTCG4500.014.57694732
GTTTTCG6050.014.54462428
TAAGACT1553.1268428E-914.4540854
TCTAGCG4900.014.366528528
CGTTATT3350.014.3289422
CGTATAT4150.014.26517710
AGCGTAT4050.014.2246548
AATAGCG4300.014.1424145
CAACGGA1258.600564E-714.07919814
Produced by FastQC (version 0.11.5)