##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062915_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1534956
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.167328574890746	32.0	32.0	32.0	32.0	32.0
2	31.296271684660667	32.0	32.0	32.0	32.0	32.0
3	31.377919627663594	32.0	32.0	32.0	32.0	32.0
4	31.477272964176173	32.0	32.0	32.0	32.0	32.0
5	31.40129033014627	32.0	32.0	32.0	32.0	32.0
6	34.922029686844446	36.0	36.0	36.0	36.0	36.0
7	34.94878289670844	36.0	36.0	36.0	36.0	36.0
8	34.893530498594096	36.0	36.0	36.0	36.0	36.0
9	34.99564287184779	36.0	36.0	36.0	36.0	36.0
10	34.85113644951386	36.0	36.0	36.0	32.0	36.0
11	35.02284690896677	36.0	36.0	36.0	36.0	36.0
12	34.91567119839266	36.0	36.0	36.0	32.0	36.0
13	34.96634626660308	36.0	36.0	36.0	36.0	36.0
14	34.91208673082486	36.0	36.0	36.0	32.0	36.0
15	34.88796616971431	36.0	36.0	36.0	32.0	36.0
16	34.88598956582469	36.0	36.0	36.0	32.0	36.0
17	34.8579535830343	36.0	36.0	36.0	32.0	36.0
18	34.84941066714616	36.0	36.0	36.0	32.0	36.0
19	34.84708551906374	36.0	36.0	36.0	32.0	36.0
20	34.82918402872786	36.0	36.0	36.0	32.0	36.0
21	34.81554520129567	36.0	36.0	36.0	32.0	36.0
22	34.80348622370934	36.0	36.0	36.0	32.0	36.0
23	34.75061565282653	36.0	36.0	36.0	32.0	36.0
24	34.71703618865948	36.0	36.0	36.0	32.0	36.0
25	34.69682388289957	36.0	36.0	36.0	32.0	36.0
26	34.632182942051756	36.0	36.0	36.0	32.0	36.0
27	34.61283320173347	36.0	36.0	36.0	32.0	36.0
28	34.58507475132838	36.0	36.0	36.0	32.0	36.0
29	34.544923763287024	36.0	36.0	36.0	32.0	36.0
30	34.51924615428716	36.0	36.0	36.0	32.0	36.0
31	34.51123875863543	36.0	36.0	36.0	32.0	36.0
32	34.48191804846523	36.0	36.0	36.0	32.0	36.0
33	34.431400639497156	36.0	36.0	36.0	32.0	36.0
34	34.424367213131845	36.0	36.0	36.0	32.0	36.0
35	34.379683196130706	36.0	36.0	36.0	32.0	36.0
36	34.35386942687608	36.0	36.0	36.0	32.0	36.0
37	34.3422977596752	36.0	36.0	36.0	32.0	36.0
38	33.90483570864572	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	10.0
21	38.0
22	119.0
23	368.0
24	1049.0
25	2623.0
26	5414.0
27	10508.0
28	18354.0
29	28815.0
30	43632.0
31	62382.0
32	89581.0
33	142323.0
34	348095.0
35	781644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.994431927611146	18.47634448815563	12.145838409926363	26.383385174306856
2	15.82527447040827	21.010374238740397	36.4167441933189	26.747607097532438
3	17.53529091387636	25.28645772256664	28.982133689825634	28.196117673731365
4	12.06667070589408	16.44519322165521	35.8211716756344	35.66696439681631
5	14.174552348440184	36.92759722597731	32.865263151037	16.032587274545506
6	33.90764010861577	35.98607643756938	16.580779073221834	13.52550438059301
7	29.718186076540654	30.788058756349564	21.210476699877326	18.28327846723246
8	28.41317090236747	32.13738225025392	19.44813082559519	20.00131602178342
9	27.916741862115114	13.729852777627569	18.318132451271723	40.035272908985604
10	16.197560688000387	26.200876776371206	30.94731328435425	26.654249251274152
11	37.74939457294997	20.89829281625053	22.106540827315982	19.24577178348352
12	25.10068758119178	23.869439055185005	28.146462481416286	22.88341088220693
13	29.710252092080875	19.034955422145927	25.151812268112096	26.102980217661102
14	24.130984862106796	19.726949827877803	24.842601351439388	31.299463958576013
15	25.460729818965493	26.51398476568709	22.527355833001074	25.497929582346334
16	26.013596489799372	25.325107250701162	23.495477066102914	25.165819193396548
17	24.17991134599298	25.484965041343205	25.006579993172444	25.328543619491374
18	25.48411811152893	23.935604668798323	26.1847896617232	24.395487557949544
19	25.746405760165114	24.743836305405498	25.468417335741222	24.041340598688173
20	26.090910749233203	23.38379732057466	25.09537732677679	25.429914603415344
21	27.734754439055216	23.646100374278074	24.14748315097185	24.47166203569486
22	26.283246088647548	23.790599724421888	24.738575397975836	25.187578788954724
23	24.334475169939946	23.3238259908766	25.697643056404296	26.64405578277916
24	24.92566562168557	24.81660712098588	25.11264166529855	25.145085592029996
25	25.140557958088642	23.834264740181137	25.236196003267853	25.788981298462367
26	24.916691368802343	24.783202113417005	25.6782772133385	24.62182930444215
27	25.879342312538355	24.189519685931955	24.562885923617255	25.368252077912434
28	24.649761947573744	23.973521065098936	25.922371716192515	25.45434527113481
29	24.699730806615953	24.294833206945345	25.81285717636206	25.19257881007664
30	24.691847844498476	24.417116842437178	26.131889122554654	24.759146190509696
31	25.31623056296076	24.391839244903437	24.524546631955573	25.767383560180228
32	24.863448854559998	24.483959149317634	24.566111341302292	26.086480654820072
33	24.501418933181146	23.882117793604507	25.392519394692748	26.2239438785216
34	25.321051548057405	24.056715632239623	25.662755153893663	24.959477665809313
35	26.32466337797305	23.875146909748555	25.385613659284047	24.414576052994352
36	24.963842611775192	24.818952465086948	24.83002770111977	25.387177222018092
37	25.897680454684043	24.7900265545071	24.810027127813434	24.502265862995422
38	24.90250522165485	24.24053098659634	25.398285551228245	25.45867824052056
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	496.0
1	396.0
2	296.0
3	296.0
4	909.5
5	1523.0
6	1523.0
7	1745.5
8	1968.0
9	1953.5
10	1939.0
11	1939.0
12	2428.0
13	2917.0
14	3617.0
15	4317.0
16	4317.0
17	6539.0
18	8761.0
19	8761.0
20	10036.0
21	11311.0
22	11334.0
23	11357.0
24	11357.0
25	12318.5
26	13280.0
27	13280.0
28	17238.0
29	21196.0
30	25940.5
31	30685.0
32	30685.0
33	40069.5
34	49454.0
35	49454.0
36	54236.5
37	59019.0
38	68360.5
39	77702.0
40	77702.0
41	83276.0
42	88850.0
43	103480.5
44	118111.0
45	118111.0
46	122078.0
47	126045.0
48	126045.0
49	135296.0
50	144547.0
51	144461.5
52	144376.0
53	144376.0
54	138480.0
55	132584.0
56	132584.0
57	130108.5
58	127633.0
59	114646.5
60	101660.0
61	101660.0
62	97822.0
63	93984.0
64	77004.5
65	60025.0
66	60025.0
67	50335.0
68	40645.0
69	40645.0
70	32711.5
71	24778.0
72	19226.0
73	13674.0
74	13674.0
75	10173.0
76	6672.0
77	6672.0
78	6588.0
79	6504.0
80	4995.5
81	3487.0
82	3487.0
83	3419.0
84	3351.0
85	3351.0
86	2160.5
87	970.0
88	807.5
89	645.0
90	645.0
91	375.0
92	105.0
93	77.5
94	50.0
95	50.0
96	35.5
97	21.0
98	21.0
99	19.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008729891931755699
2	0.0
3	0.0
4	5.863360252671738E-4
5	6.514844725190819E-5
6	2.6059378900763276E-4
7	4.5603913076335743E-4
8	0.0017590080758015214
9	0.004364945965877849
10	9.77226708778623E-4
11	0.005798211805419829
12	6.514844725190819E-4
13	6.514844725190819E-5
14	0.0
15	0.0
16	6.514844725190819E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	6.514844725190819E-5
22	6.514844725190819E-5
23	1.3029689450381638E-4
24	0.0
25	1.3029689450381638E-4
26	6.514844725190819E-5
27	1.3029689450381638E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.3029689450381638E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1534956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.452182295963084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.68108711005034	37.15366011308784
2	14.078734819764483	13.642908519675034
3	4.222504852131516	6.137687245231946
4	1.7425524278511157	3.3772187157806126
5	0.8915585468965349	2.159897862087743
6	0.5441524923468749	1.5819225453596062
7	0.34536993784542025	1.171374903162222
8	0.23945267433963172	0.9281603702687774
9	0.18738658966221194	0.8171360281919091
>10	0.857339685026329	7.455628171886672
>50	0.08402703837364606	2.9055605971205747
>100	0.1016142588740608	10.917874506106092
>500	0.01650719080563223	5.548972849799987
>1k	0.007577071189470531	5.79350474566438
>5k	1.353048426691166E-4	0.40849282657668434
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6231	0.40593997482664	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4490	0.29251652816106777	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2715	0.17687803428893076	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2466	0.16065607092320563	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2397	0.15616082806282394	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2254	0.14684460010580108	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2120	0.13811470817404536	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2114	0.13772381749053395	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2086	0.13589966096748052	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2029	0.13218619947412172	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2007	0.13075293363457977	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2000	0.13029689450381637	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1985	0.12931966779503776	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1949	0.12697432369396908	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1885	0.12280482306984694	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1863	0.12137155723030497	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1830	0.119221658470992	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1796	0.11700661126442713	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1767	0.11511730629412179	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1747	0.11381433734908362	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1689	0.11003572740847295	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1684	0.1097099851722134	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1618	0.10541018765358746	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1597	0.10404207026129739	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1585	0.10326028889427451	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1578	0.10280424976351113	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1572	0.10241335907999968	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1545	0.10065435100419817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.302968945038164E-4
2	0.0	0.0	0.0	0.0	1.302968945038164E-4
3	0.0	0.0	0.0	6.51484472519082E-5	1.302968945038164E-4
4	0.0	0.0	0.0	6.51484472519082E-5	1.302968945038164E-4
5	0.0	0.0	0.0	6.51484472519082E-5	1.954453417557246E-4
6	0.0	0.0	0.0	6.51484472519082E-5	1.954453417557246E-4
7	0.0	0.0	0.0	6.51484472519082E-5	1.954453417557246E-4
8	0.0	0.0	0.0	6.51484472519082E-5	1.954453417557246E-4
9	0.0	0.0	0.0	1.302968945038164E-4	1.954453417557246E-4
10	0.0	0.0	0.0	1.302968945038164E-4	2.605937890076328E-4
11	0.0	0.0	0.0	1.302968945038164E-4	2.605937890076328E-4
12	0.0	0.0	0.0	1.302968945038164E-4	3.908906835114492E-4
13	0.0	0.0	0.0	1.302968945038164E-4	3.908906835114492E-4
14	0.0	0.0	0.0	1.302968945038164E-4	3.908906835114492E-4
15	0.0	0.0	0.0	1.302968945038164E-4	4.560391307633574E-4
16	0.0	0.0	0.0	1.302968945038164E-4	4.560391307633574E-4
17	0.0	0.0	0.0	1.302968945038164E-4	4.560391307633574E-4
18	0.0	0.0	0.0	1.954453417557246E-4	4.560391307633574E-4
19	0.0	0.0	0.0	2.605937890076328E-4	4.560391307633574E-4
20	0.0	0.0	0.0	5.211875780152656E-4	4.560391307633574E-4
21	0.0	0.0	0.0	7.166329197709902E-4	4.560391307633574E-4
22	0.0	0.0	0.0	0.0010423751560305313	4.560391307633574E-4
23	0.0	0.0	0.0	0.0016938596285496132	4.560391307633574E-4
24	0.0	0.0	0.0	0.0031271254680915934	5.211875780152656E-4
25	0.0	0.0	0.0	0.0037786099406106753	5.211875780152656E-4
26	0.0	0.0	0.0	0.00469068820213739	5.211875780152656E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACT	80	5.61461E-5	16.002737	4
CATCGTT	440	0.0	15.635471	28
GTATAAT	165	2.910383E-11	15.515784	1
TAATACC	155	1.891749E-10	15.48652	4
ATCGTTT	435	0.0	15.447395	29
TCCAATA	450	0.0	15.289513	2
TAGCGTA	385	0.0	14.963598	7
GCGACGC	75	6.248746E-4	14.932482	32
ACCGTCG	355	0.0	14.875784	8
GTTATTC	340	0.0	14.590256	3
TAAACGC	450	0.0	14.576947	28
CGCTTCG	450	0.0	14.576947	32
GTTTTCG	605	0.0	14.544624	28
TAAGACT	155	3.1268428E-9	14.454085	4
TCTAGCG	490	0.0	14.3665285	28
CGTTATT	335	0.0	14.328942	2
CGTATAT	415	0.0	14.265177	10
AGCGTAT	405	0.0	14.224654	8
AATAGCG	430	0.0	14.142414	5
CAACGGA	125	8.600564E-7	14.079198	14
>>END_MODULE