FastQCFastQC Report
Thu 2 Feb 2017
SRR4062914_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062914_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551125
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38940.7065547743252438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28190.5114992061691994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16280.2953957813563166No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12350.22408709457926967No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA11580.21011567248809254No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11460.20793830800635063No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9920.1799954638239964No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8940.1622136538897709No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA6960.12628713994102972No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG6570.11921070537536857No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA6470.11739623497391699No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC5820.10560217736448174No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT5780.1048763892039011No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG5750.10433204808346563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG200.003737885532.0243451
TGTAGGA1200.021.3379332
ATCCACG400.004460886620.0152171
TAGCGTA801.2632518E-719.9988657
TAGGACG801.2681085E-719.9916064
GTGTAGG751.4947254E-619.2146071
CTAGGAC1003.265086E-919.1936843
CGAGGTT751.5117221E-619.1919424
TAGGAGT507.2097685E-419.1919424
AGCGTAT852.3917892E-718.822468
AGCGGCG1208.185452E-1118.66730131
CTAGCGG1208.185452E-1118.66730129
TAGCGGC1159.367795E-1018.08757230
AGGACGT802.8020331E-617.9924455
GTATAGT450.00880591817.7913021
CGTATAT904.35497E-717.77838310
AAGACCG450.00886600417.7703175
ATGCACT450.00886600417.7703174
CGAAAAC1453.6379788E-1217.65577323
GATATAC1006.7362635E-817.613391
Produced by FastQC (version 0.11.5)