##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062914_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	551125
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.091331367657066	32.0	32.0	32.0	32.0	32.0
2	30.774659106373328	32.0	32.0	32.0	32.0	32.0
3	30.86886278067589	32.0	32.0	32.0	32.0	32.0
4	30.885352687684282	32.0	32.0	32.0	32.0	32.0
5	30.87121251984577	32.0	32.0	32.0	32.0	32.0
6	34.48068586981175	36.0	36.0	36.0	32.0	36.0
7	34.36502608301202	36.0	36.0	36.0	32.0	36.0
8	34.33881242912225	36.0	36.0	36.0	32.0	36.0
9	34.41154547516444	36.0	36.0	36.0	32.0	36.0
10	34.208469947833976	36.0	36.0	36.0	32.0	36.0
11	34.482253572238605	36.0	36.0	36.0	32.0	36.0
12	34.31317940576094	36.0	36.0	36.0	32.0	36.0
13	34.379861193014285	36.0	36.0	36.0	32.0	36.0
14	34.30209662054888	36.0	36.0	36.0	32.0	36.0
15	34.27284735767748	36.0	36.0	36.0	32.0	36.0
16	34.27919618961216	36.0	36.0	36.0	32.0	36.0
17	34.20160762077569	36.0	36.0	36.0	32.0	36.0
18	34.224471762304375	36.0	36.0	36.0	32.0	36.0
19	34.20741211158993	36.0	36.0	36.0	32.0	36.0
20	34.14907507371286	36.0	36.0	36.0	32.0	36.0
21	34.11979859378544	36.0	36.0	36.0	32.0	36.0
22	34.09943116352915	36.0	36.0	36.0	32.0	36.0
23	34.05968337491495	36.0	36.0	36.0	32.0	36.0
24	34.05159265139488	36.0	36.0	36.0	32.0	36.0
25	34.04017418915854	36.0	36.0	36.0	32.0	36.0
26	33.993146745293714	36.0	36.0	36.0	32.0	36.0
27	34.00816330233613	36.0	36.0	36.0	32.0	36.0
28	33.989927874801545	36.0	36.0	36.0	32.0	36.0
29	33.94961215695169	36.0	36.0	36.0	32.0	36.0
30	33.9133753685643	36.0	36.0	36.0	32.0	36.0
31	33.929221138580175	36.0	36.0	36.0	32.0	36.0
32	33.86841097754593	36.0	36.0	36.0	32.0	36.0
33	33.81595645271037	36.0	36.0	36.0	32.0	36.0
34	33.79383805851667	36.0	36.0	36.0	32.0	36.0
35	33.73536493535949	36.0	36.0	36.0	27.0	36.0
36	33.68936811068269	36.0	36.0	36.0	27.0	36.0
37	33.675534588342025	36.0	36.0	36.0	27.0	36.0
38	33.043481968700384	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	2.0
9	2.0
10	2.0
11	9.0
12	4.0
13	1.0
14	260.0
15	416.0
16	465.0
17	507.0
18	558.0
19	698.0
20	900.0
21	1073.0
22	1504.0
23	2107.0
24	2897.0
25	4009.0
26	5634.0
27	8084.0
28	10975.0
29	14447.0
30	19360.0
31	25858.0
32	34705.0
33	51411.0
34	113572.0
35	251662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.340240459554614	17.460750009991898	11.758464953873768	24.440544576579722
2	16.024284059591803	20.66287349268537	38.254111188168785	25.05873125955404
3	18.76231712584065	25.767880928098837	28.807829246073947	26.66197269998657
4	12.046575009390123	16.06699920705629	36.1406322864178	35.745793497135786
5	13.89891893265012	37.655001977783506	33.16652211307115	15.279556976495224
6	32.509439816520086	36.13222456692971	17.616212714766032	13.742122901784176
7	29.045679292356542	31.6440009072352	21.168156044454527	18.14216375595373
8	28.50050805632167	32.40709827260851	19.859921614167515	19.23247205690231
9	28.224059282699653	14.09760452313644	18.913053734018963	38.76528246014494
10	16.077986645376686	27.23508491798519	31.463928001161275	25.223000435476845
11	36.633255551482996	21.92849392321734	22.201395718490186	19.236854806809482
12	24.79786176212285	24.861007279879807	28.838267635381815	21.502863322615532
13	30.012213291618195	20.128702324154915	25.42306442919648	24.436019955030407
14	24.0559344117124	20.273502288258925	26.144401659305284	29.526161640723387
15	25.067876588021782	27.06987295825771	24.19419237749546	23.668058076225044
16	24.496887872684052	26.496089426025733	24.986843776652695	24.020178924637523
17	23.07847265072048	26.09052303001706	26.312656527893726	24.518347791368733
18	23.891430303052296	25.035701778102787	27.917248086983506	23.15561983186141
19	24.763098966744938	25.53631635678693	27.0718633069243	22.628721369543836
20	25.204594530838882	24.086809776987426	27.054927507303706	23.653668184869986
21	26.69819229666513	24.304917925695808	25.65026148368857	23.34662829395049
22	25.328535499254194	24.629909886369	26.28483601351523	23.75671860086158
23	23.544302419267034	24.628000493579833	26.91715843186784	24.910538655285297
24	23.635093322686895	26.091154079975322	26.70725621319493	23.56649638414286
25	24.044745213971627	25.0577553712949	26.83170275356559	24.065796661167884
26	23.440848896865997	25.892661078252914	27.37826689000964	23.288223134871448
27	24.374854828407177	25.538949538354334	26.399272690319957	23.686922942918528
28	23.33216594778702	25.666271691142445	27.344947912224704	23.65661444884583
29	23.215160654595344	25.75518366487738	27.404737795295596	23.62491788523168
30	23.46866749530489	25.98542927391332	27.508369548452656	23.03753368232914
31	23.699438747643303	26.04715390798945	26.02429008744504	24.2291172569222
32	23.1710592214617	26.234657511377574	26.145741846977955	24.448541420182767
33	23.11772444767046	25.68978814136007	26.724855963344375	24.467631447625095
34	23.69490301260682	26.092140291914905	26.889707123297118	23.323249572181158
35	24.457563263113578	25.879985191422637	26.726034059727926	22.936417485735856
36	23.315996355968192	26.790711348400652	26.113262606208647	23.78002968942251
37	23.985693183756574	26.810037273212284	26.2329739087514	22.97129563427974
38	23.31949394284719	26.67324763556917	26.28474062030471	23.722517801278933
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1034.0
1	744.0
2	454.0
3	454.0
4	882.5
5	1311.0
6	1311.0
7	1494.5
8	1678.0
9	1564.5
10	1451.0
11	1451.0
12	1861.5
13	2272.0
14	2633.5
15	2995.0
16	2995.0
17	4328.0
18	5661.0
19	5661.0
20	6281.0
21	6901.0
22	6502.5
23	6104.0
24	6104.0
25	6465.5
26	6827.0
27	6827.0
28	8946.0
29	11065.0
30	13237.0
31	15409.0
32	15409.0
33	17856.0
34	20303.0
35	20303.0
36	22078.5
37	23854.0
38	27178.5
39	30503.0
40	30503.0
41	32732.0
42	34961.0
43	38930.5
44	42900.0
45	42900.0
46	45793.0
47	48686.0
48	48686.0
49	49651.0
50	50616.0
51	49021.0
52	47426.0
53	47426.0
54	44636.0
55	41846.0
56	41846.0
57	40377.0
58	38908.0
59	34718.5
60	30529.0
61	30529.0
62	28917.0
63	27305.0
64	22612.0
65	17919.0
66	17919.0
67	15015.0
68	12111.0
69	12111.0
70	9861.5
71	7612.0
72	5991.5
73	4371.0
74	4371.0
75	3400.5
76	2430.0
77	2430.0
78	2243.5
79	2057.0
80	1597.0
81	1137.0
82	1137.0
83	1173.5
84	1210.0
85	1210.0
86	833.5
87	457.0
88	381.0
89	305.0
90	305.0
91	208.5
92	112.0
93	98.5
94	85.0
95	85.0
96	71.0
97	57.0
98	57.0
99	160.0
100	263.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12320254025856203
2	0.05643002948514402
3	0.011431163529144932
4	0.002540258562032207
5	5.443411204354728E-4
6	3.628940802903153E-4
7	0.0
8	9.072352007257881E-4
9	5.443411204354728E-4
10	9.072352007257881E-4
11	0.001995917441596734
12	0.00308459968246768
13	0.015604445452483556
14	0.009253799047403038
15	0.022680880018144705
16	0.00997958720798367
17	0.01905193921524155
18	0.005806305284645045
19	0.009253799047403038
20	0.0063506464050805175
21	0.007076434565661148
22	0.007802222726241778
23	0.008890904967112723
24	0.01451576321161261
25	0.017056021773644816
26	0.01887049217509639
27	0.009616693127693353
28	0.006532093445225675
29	0.012156951689725562
30	0.0036289408029031526
31	0.006532093445225675
32	0.007439328645951463
33	0.00907235200725788
34	0.013427080970741665
35	0.01687457473349966
36	0.015785892492628715
37	0.0107053753685643
38	0.007439328645951463
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	551125.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.18777952696259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.40435262494536	49.59735007852293
2	11.715105470497434	14.10212370386826
3	2.917290048722729	5.267556308061767
4	1.0357376499011373	2.493549972800961
5	0.5108006275703301	1.5371977777218575
6	0.26153933867357254	0.9444883232227551
7	0.17586515811504336	0.7409453354171851
8	0.10731028967487334	0.5167014444740613
9	0.087882761036805	0.47605214209535623
>10	0.6046968410786254	7.328947498121499
>50	0.09333422677299025	4.075926835682156
>100	0.07974186047351017	9.062483088991158
>500	0.004530787526903949	1.6969090082578002
>1k	0.0018123150107615792	2.1597684827622308
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3894	0.7065547743252438	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2819	0.5114992061691994	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1628	0.2953957813563166	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1235	0.22408709457926967	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1158	0.21011567248809254	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1146	0.20793830800635063	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	992	0.1799954638239964	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	894	0.1622136538897709	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	696	0.12628713994102972	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	657	0.11921070537536857	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	647	0.11739623497391699	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	582	0.10560217736448174	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	578	0.1048763892039011	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	575	0.10433204808346563	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.8144704014515762E-4
13	0.0	0.0	0.0	0.0	1.8144704014515762E-4
14	0.0	0.0	0.0	1.8144704014515762E-4	1.8144704014515762E-4
15	0.0	0.0	0.0	1.8144704014515762E-4	3.6289408029031525E-4
16	0.0	0.0	0.0	3.6289408029031525E-4	3.6289408029031525E-4
17	0.0	0.0	0.0	5.443411204354728E-4	3.6289408029031525E-4
18	0.0	0.0	0.0	5.443411204354728E-4	3.6289408029031525E-4
19	0.0	0.0	0.0	5.443411204354728E-4	3.6289408029031525E-4
20	0.0	0.0	0.0	7.257881605806305E-4	3.6289408029031525E-4
21	0.0	0.0	0.0	9.072352007257881E-4	3.6289408029031525E-4
22	0.0	0.0	0.0	0.0016330233613064188	3.6289408029031525E-4
23	0.0	0.0	0.0	0.004717623043774099	3.6289408029031525E-4
24	0.0	0.0	0.0	0.00762077568609662	3.6289408029031525E-4
25	0.0	0.0	0.0	0.009435246087548197	3.6289408029031525E-4
26	0.0	0.0	0.0	0.011612610569290088	3.6289408029031525E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	20	0.0037378855	32.024345	1
TGTAGGA	120	0.0	21.337933	2
ATCCACG	40	0.0044608866	20.015217	1
TAGCGTA	80	1.2632518E-7	19.998865	7
TAGGACG	80	1.2681085E-7	19.991606	4
GTGTAGG	75	1.4947254E-6	19.214607	1
CTAGGAC	100	3.265086E-9	19.193684	3
CGAGGTT	75	1.5117221E-6	19.191942	4
TAGGAGT	50	7.2097685E-4	19.191942	4
AGCGTAT	85	2.3917892E-7	18.82246	8
AGCGGCG	120	8.185452E-11	18.667301	31
CTAGCGG	120	8.185452E-11	18.667301	29
TAGCGGC	115	9.367795E-10	18.087572	30
AGGACGT	80	2.8020331E-6	17.992445	5
GTATAGT	45	0.008805918	17.791302	1
CGTATAT	90	4.35497E-7	17.778383	10
AAGACCG	45	0.008866004	17.770317	5
ATGCACT	45	0.008866004	17.770317	4
CGAAAAC	145	3.6379788E-12	17.655773	23
GATATAC	100	6.7362635E-8	17.61339	1
>>END_MODULE