FastQCFastQC Report
Thu 2 Feb 2017
SRR4062914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551125
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT48020.871308686777047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35350.6414152869131323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22710.41206622816965294No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15150.2748922658199138No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12270.2226355182581084No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11830.2146518484917215No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9950.18053980494443184No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.14552052619641642No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.1319119981855296No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.11757768201406216No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG6310.11449308233159447No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC5980.10850533000680426No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC5900.107053753685643No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT5860.10632796552506238No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC5830.10578362440462691No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA5690.10324336584259469No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG5630.10215468360172375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTA356.427528E-527.42678329
TAGGATT401.5908288E-424.002794
CTATAAG350.002063788722.85981
GTCGTAA350.002065967522.85565430
TACCGTC1800.020.4486777
CGTAAAC400.004482260419.99869532
CGGTCTG400.004482260419.99869525
ACCGTCG1700.019.7687998
CTATACT659.409758E-619.6945954
CCCGTGC507.1794295E-419.2039748
CCGTCGT1750.019.2004899
AGGTCGT507.192594E-419.19874828
ATACCGT1850.019.0309666
CGTCGTA1850.019.02751210
GAGGTCG601.1373855E-418.66545127
CGGTCCA1401.8189894E-1218.2861810
GTCGTAG1850.018.16262411
GATATAC802.7877286E-618.0020941
CATCGTT1006.80011E-817.59885228
ACTAGAC550.001362362317.4565753
Produced by FastQC (version 0.11.5)