FastQCFastQC Report
Thu 2 Feb 2017
SRR4062913_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062913_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501230
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT53340.3553086469095342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34020.22661417637537218No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21910.14594699013475615No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21320.14201687949214975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20840.1388195013422327No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18430.12276599854785743No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17530.11677091451676293No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17370.11570512180012389No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17350.11557189771054402No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17230.11477255317306476No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17180.11443949294911507No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17100.11390659659079555No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15210.1013169201254971No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC15120.10071741172238764No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15120.10071741172238764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3700.020.75662628
CTAGCGG4000.019.59987829
TAGCGGC4250.018.44694330
GTTCAAA4850.018.1623691
GTATACG550.0013558517.4718991
TAAACGC4200.016.76179928
CGCAAGA4650.016.5209792
GCGCAAG4850.016.5112441
AGCGGCG4750.016.5051631
CAAGACG4800.016.3305114
TACCGTC3750.015.7860427
AAGACGG4900.015.6697175
GTATTAG4000.015.615511
AAACGCT4050.015.407310529
ACCGTCG3850.015.3760168
ATACCGT3850.015.3729426
CGTCTTA1255.140282E-815.35990415
GTTTTCG4800.015.33323928
GTCATAT1153.3092329E-715.3195621
GTATTAT1153.3092329E-715.3195621
Produced by FastQC (version 0.11.5)