##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062913_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1501230
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229032193601245	32.0	32.0	32.0	32.0	32.0
2	30.836580004396396	32.0	32.0	32.0	32.0	32.0
3	30.882511007640403	32.0	32.0	32.0	32.0	32.0
4	30.950803008199944	32.0	32.0	32.0	32.0	32.0
5	30.854863678450336	32.0	32.0	32.0	32.0	32.0
6	34.51210207629744	36.0	36.0	36.0	32.0	36.0
7	34.4349446786968	36.0	36.0	36.0	32.0	36.0
8	34.408890043497664	36.0	36.0	36.0	32.0	36.0
9	34.55905624054942	36.0	36.0	36.0	32.0	36.0
10	34.26870765971903	36.0	36.0	36.0	32.0	36.0
11	34.56830931969119	36.0	36.0	36.0	32.0	36.0
12	34.366133770308345	36.0	36.0	36.0	32.0	36.0
13	34.45921344497512	36.0	36.0	36.0	32.0	36.0
14	34.352828680482006	36.0	36.0	36.0	32.0	36.0
15	34.29569353130433	36.0	36.0	36.0	32.0	36.0
16	34.31080980262851	36.0	36.0	36.0	32.0	36.0
17	34.226577539750735	36.0	36.0	36.0	32.0	36.0
18	34.2611225461788	36.0	36.0	36.0	32.0	36.0
19	34.24820713681448	36.0	36.0	36.0	32.0	36.0
20	34.22280863025652	36.0	36.0	36.0	32.0	36.0
21	34.21140264982714	36.0	36.0	36.0	32.0	36.0
22	34.18174097240263	36.0	36.0	36.0	32.0	36.0
23	34.13013262458118	36.0	36.0	36.0	32.0	36.0
24	34.114230331128475	36.0	36.0	36.0	32.0	36.0
25	34.094533815604535	36.0	36.0	36.0	32.0	36.0
26	34.05014288283608	36.0	36.0	36.0	32.0	36.0
27	34.053814538744895	36.0	36.0	36.0	32.0	36.0
28	34.04124351365214	36.0	36.0	36.0	32.0	36.0
29	34.000300420322006	36.0	36.0	36.0	32.0	36.0
30	33.98135728702464	36.0	36.0	36.0	32.0	36.0
31	33.99853520113507	36.0	36.0	36.0	32.0	36.0
32	33.94222604131279	36.0	36.0	36.0	32.0	36.0
33	33.90437308074046	36.0	36.0	36.0	32.0	36.0
34	33.90455293326139	36.0	36.0	36.0	32.0	36.0
35	33.86845120334659	36.0	36.0	36.0	32.0	36.0
36	33.811788333566476	36.0	36.0	36.0	32.0	36.0
37	33.82142842868848	36.0	36.0	36.0	32.0	36.0
38	33.21129273995324	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	2.0
9	4.0
10	15.0
11	12.0
12	7.0
13	8.0
14	147.0
15	364.0
16	482.0
17	575.0
18	718.0
19	941.0
20	1338.0
21	1980.0
22	3184.0
23	4627.0
24	6885.0
25	10385.0
26	15112.0
27	21375.0
28	30301.0
29	39926.0
30	54003.0
31	72143.0
32	98565.0
33	147214.0
34	323972.0
35	666940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.04501367536986	16.942119272167538	11.447628435432312	25.56523861703029
2	16.955800482670966	19.05204008794982	37.54309681211889	26.44906261726032
3	19.253168756520385	23.31116236371316	28.105701163576185	29.329967716190268
4	12.60132002472715	14.758950268587995	35.39299646145975	37.2467332452251
5	14.807364114397167	36.30299501873802	33.15443510837142	15.735205758493393
6	35.09438637697397	35.01764894356135	16.284722522902992	13.603242156561684
7	31.19237637961963	30.136841214764704	20.040913145163064	18.6298692604526
8	28.934475424370664	32.34209707685001	18.744783419918292	19.978644078861034
9	27.7052407200786	13.929757364818732	18.327965494845877	40.03703642025679
10	16.32152515953691	25.989994804225898	30.816469271659052	26.872010764578143
11	38.13221796119512	21.015878598697178	21.502355111200817	19.34954832890688
12	25.5080302959652	23.4957600303759	27.868490997142263	23.127718676516633
13	29.700327846572332	19.039684691676985	25.01833768929792	26.241649772452757
14	23.99442803410331	19.536055895531636	24.597125014822602	31.872391055542455
15	25.506356650713684	26.635435870818842	22.327265071102143	25.53094240736533
16	26.21892764612575	25.59629806542474	23.262403126338643	24.922371162110863
17	24.313703804136	25.686929141412694	24.74963672256797	25.249730331883335
18	25.318905293338247	24.370499703227726	26.018368571849198	24.292226431584833
19	25.737083960088412	24.663284298769316	25.253910751852953	24.345720989289315
20	26.091263364753686	23.77743729807148	24.97745062119042	25.153848715984413
21	27.509614378967413	23.61716485570604	24.352407541750022	24.52081322357653
22	26.40063845704438	23.64469439131741	24.93932859420206	25.015338557436152
23	24.537248921940712	23.840815627712637	25.6577777156002	25.96415773474645
24	25.006479001041303	24.905146758534126	24.96623924136061	25.12213499906396
25	25.168676167741427	24.21861310780551	25.147556252594182	25.46515447185888
26	24.8792581128088	25.303795182850088	25.516196968677402	24.30074973566371
27	25.581190847505724	24.587319024261557	24.796499098327292	25.034991029905424
28	24.747343198234418	24.283031575188836	25.641859191671436	25.32776603490531
29	24.52238528552956	24.72337752231267	25.736066614569907	25.018170577587867
30	24.506173456302854	24.920446180850462	25.86490006228413	24.708480300562556
31	25.228975520653265	24.788910849932353	24.54016705936743	25.44194657004696
32	25.006761536913995	24.60866356057871	24.449117935859327	25.93545696664797
33	24.555693084147514	24.376158315690738	25.157384374945874	25.91076422521588
34	25.511442672053125	24.556176751176512	25.19279863135623	24.739581945414137
35	25.849247423010098	24.23116692072358	25.34970579143171	24.569879864834615
36	24.493431633423985	25.209429560114643	24.897100698076326	25.400038108385044
37	25.756333226966255	24.98143017312075	24.568197397759363	24.694039202153633
38	24.719961149293432	24.850729216837173	25.14204289965366	25.287266734215734
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	95.0
1	128.5
2	162.0
3	162.0
4	449.5
5	737.0
6	737.0
7	1040.5
8	1344.0
9	1380.0
10	1416.0
11	1416.0
12	1921.0
13	2426.0
14	3112.5
15	3799.0
16	3799.0
17	5567.5
18	7336.0
19	7336.0
20	8859.0
21	10382.0
22	10435.5
23	10489.0
24	10489.0
25	11874.0
26	13259.0
27	13259.0
28	17511.0
29	21763.0
30	26824.0
31	31885.0
32	31885.0
33	40287.5
34	48690.0
35	48690.0
36	53754.0
37	58818.0
38	67800.5
39	76783.0
40	76783.0
41	82224.5
42	87666.0
43	100455.0
44	113244.0
45	113244.0
46	116861.5
47	120479.0
48	120479.0
49	128487.5
50	136496.0
51	139053.5
52	141611.0
53	141611.0
54	136470.5
55	131330.0
56	131330.0
57	128065.5
58	124801.0
59	112595.0
60	100389.0
61	100389.0
62	94828.5
63	89268.0
64	75090.5
65	60913.0
66	60913.0
67	51282.0
68	41651.0
69	41651.0
70	33178.5
71	24706.0
72	19331.0
73	13956.0
74	13956.0
75	10753.0
76	7550.0
77	7550.0
78	7047.5
79	6545.0
80	5163.5
81	3782.0
82	3782.0
83	3645.0
84	3508.0
85	3508.0
86	2444.5
87	1381.0
88	1127.0
89	873.0
90	873.0
91	648.0
92	423.0
93	374.0
94	325.0
95	325.0
96	300.0
97	275.0
98	275.0
99	474.5
100	674.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12116730947289889
2	0.055221385130859356
3	0.011190823524709738
4	0.0030641540603371905
5	2.6644817915975563E-4
6	4.6628431352957245E-4
7	6.661204478993891E-5
8	4.6628431352957245E-4
9	3.330602239496946E-4
10	6.661204478993892E-4
11	0.001798525209328351
12	0.002664481791597557
13	0.014854485988156379
14	0.009592134449751204
15	0.02404694816916795
16	0.011790331927819189
17	0.020716345929671
18	0.005262351538405174
19	0.007460549016473159
20	0.005328963583195114
21	0.006194920165464319
22	0.007393936971683219
23	0.010191642852860654
24	0.015120934167316132
25	0.01845153640681308
26	0.020050225481771614
27	0.008726177867481998
28	0.00552879971756493
29	0.011790331927819189
30	0.002997542015547251
31	0.005795247896724686
32	0.006128308120674381
33	0.00852634173311218
34	0.012123392151768882
35	0.016919459376644484
36	0.01651978710790485
37	0.00925907422580151
38	0.006594592434203952
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1501230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.01047671474417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.16313066781109	36.34770388687285
2	14.430703281306922	14.145112942917503
3	5.149930609022475	7.572016625881329
4	2.2958569586160307	4.500841760425374
5	1.1781275162189	2.887029560032289
6	0.7020694693652424	2.0645255628274803
7	0.40998390399978013	1.4065454606280792
8	0.29684123843009597	1.1638664483243233
9	0.20046369049731338	0.8842338931743233
>10	0.9761195205776588	8.588255680252987
>50	0.09056228166768383	3.125323502874336
>100	0.08887317428647876	9.31632590882006
>500	0.012013516233291433	3.924072347261299
>1k	0.005187654737103119	3.7172605068994056
>5k	1.3651722992376629E-4	0.3568859128082622
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5334	0.3553086469095342	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3402	0.22661417637537218	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2191	0.14594699013475615	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2132	0.14201687949214975	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2084	0.1388195013422327	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1843	0.12276599854785743	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1753	0.11677091451676293	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1737	0.11570512180012389	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1735	0.11557189771054402	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1723	0.11477255317306476	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1718	0.11443949294911507	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1710	0.11390659659079555	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1521	0.1013169201254971	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1512	0.10071741172238764	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1512	0.10071741172238764	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	6.661204478993892E-5	0.0	0.0	0.0	0.0
11	6.661204478993892E-5	0.0	0.0	0.0	0.0
12	6.661204478993892E-5	0.0	0.0	0.0	6.661204478993892E-5
13	6.661204478993892E-5	0.0	0.0	0.0	1.9983613436981676E-4
14	6.661204478993892E-5	0.0	0.0	0.0	1.9983613436981676E-4
15	1.3322408957987784E-4	0.0	0.0	0.0	1.9983613436981676E-4
16	1.3322408957987784E-4	0.0	0.0	6.661204478993892E-5	1.9983613436981676E-4
17	1.9983613436981676E-4	0.0	0.0	6.661204478993892E-5	1.9983613436981676E-4
18	1.9983613436981676E-4	0.0	0.0	1.9983613436981676E-4	1.9983613436981676E-4
19	1.9983613436981676E-4	0.0	0.0	1.9983613436981676E-4	1.9983613436981676E-4
20	1.9983613436981676E-4	0.0	0.0	4.662843135295724E-4	2.664481791597557E-4
21	1.9983613436981676E-4	0.0	0.0	4.662843135295724E-4	3.330602239496946E-4
22	1.9983613436981676E-4	0.0	0.0	5.328963583195114E-4	3.330602239496946E-4
23	1.9983613436981676E-4	0.0	0.0	9.325686270591448E-4	3.330602239496946E-4
24	2.664481791597557E-4	0.0	0.0	0.0019983613436981673	3.330602239496946E-4
25	2.664481791597557E-4	0.0	0.0	0.0021981974780679843	3.330602239496946E-4
26	2.664481791597557E-4	0.0	0.0	0.0027977058811774346	3.330602239496946E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	370	0.0	20.756626	28
CTAGCGG	400	0.0	19.599878	29
TAGCGGC	425	0.0	18.446943	30
GTTCAAA	485	0.0	18.162369	1
GTATACG	55	0.00135585	17.471899	1
TAAACGC	420	0.0	16.761799	28
CGCAAGA	465	0.0	16.520979	2
GCGCAAG	485	0.0	16.511244	1
AGCGGCG	475	0.0	16.50516	31
CAAGACG	480	0.0	16.330511	4
TACCGTC	375	0.0	15.786042	7
AAGACGG	490	0.0	15.669717	5
GTATTAG	400	0.0	15.61551	1
AAACGCT	405	0.0	15.4073105	29
ACCGTCG	385	0.0	15.376016	8
ATACCGT	385	0.0	15.372942	6
CGTCTTA	125	5.140282E-8	15.359904	15
GTTTTCG	480	0.0	15.333239	28
GTCATAT	115	3.3092329E-7	15.319562	1
GTATTAT	115	3.3092329E-7	15.319562	1
>>END_MODULE