FastQCFastQC Report
Thu 2 Feb 2017
SRR4062913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501230
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT58930.39254477994711007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT40550.2701118416232023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT26010.17325792849863111No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22910.15260819461375008No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22720.15134256576274122No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18820.12536386829466506No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18410.12263277445827754No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18190.12116730947289889No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17330.11543867362096415No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17270.1150390013522245No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17210.11463932908348487No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17080.11377337250121568No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16810.11197484729188732No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16480.10977664981381932No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15310.1019830405733965No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15220.10138353217028703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC659.4343395E-619.6929443
TACCGTC3400.017.8841237
ACCGTCG3650.017.5353978
CGTCGTA3750.016.6410910
TCTAGCG4150.016.57774428
ATACCGT3700.016.433516
TAGCGGC4300.016.3715330
AACCGCG2350.016.3420417
CTAGCGG4250.016.1876829
TACGGAC703.6973695E-415.99998313
CGCAAGA5050.015.8420952
CCGTCGT3950.015.7985049
GTCGTAG3900.015.59076511
AAGACGG5150.015.5349995
ATTACAC1255.1379175E-815.3604963
CGATAAC2200.015.27372910
CCGATAA2350.014.9797069
GCGCAAG5350.014.9542521
ACGAACG2250.014.9328215
CCCCTAT650.00416055414.77021
Produced by FastQC (version 0.11.5)