##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062913_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1501230
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19073692905151	32.0	32.0	32.0	32.0	32.0
2	31.306146293372766	32.0	32.0	32.0	32.0	32.0
3	31.38325306581936	32.0	32.0	32.0	32.0	32.0
4	31.48555850868954	32.0	32.0	32.0	32.0	32.0
5	31.397661251107426	32.0	32.0	32.0	32.0	32.0
6	34.93057559467903	36.0	36.0	36.0	36.0	36.0
7	34.95582955309979	36.0	36.0	36.0	36.0	36.0
8	34.89158156977945	36.0	36.0	36.0	32.0	36.0
9	34.99797166323614	36.0	36.0	36.0	36.0	36.0
10	34.85117936625301	36.0	36.0	36.0	32.0	36.0
11	35.02531124477928	36.0	36.0	36.0	36.0	36.0
12	34.9272083558149	36.0	36.0	36.0	32.0	36.0
13	34.98221591628198	36.0	36.0	36.0	36.0	36.0
14	34.91785069576281	36.0	36.0	36.0	32.0	36.0
15	34.894252046655076	36.0	36.0	36.0	32.0	36.0
16	34.899591668165435	36.0	36.0	36.0	32.0	36.0
17	34.86781705668019	36.0	36.0	36.0	32.0	36.0
18	34.869314495447064	36.0	36.0	36.0	32.0	36.0
19	34.85433211433291	36.0	36.0	36.0	32.0	36.0
20	34.84049546038915	36.0	36.0	36.0	32.0	36.0
21	34.839624174843294	36.0	36.0	36.0	32.0	36.0
22	34.82280263517249	36.0	36.0	36.0	32.0	36.0
23	34.766402882969295	36.0	36.0	36.0	32.0	36.0
24	34.74018504826043	36.0	36.0	36.0	32.0	36.0
25	34.728930943293165	36.0	36.0	36.0	32.0	36.0
26	34.660812134050076	36.0	36.0	36.0	32.0	36.0
27	34.64604757432239	36.0	36.0	36.0	32.0	36.0
28	34.61605217055348	36.0	36.0	36.0	32.0	36.0
29	34.58385723706561	36.0	36.0	36.0	32.0	36.0
30	34.55949987676772	36.0	36.0	36.0	32.0	36.0
31	34.55135988489439	36.0	36.0	36.0	32.0	36.0
32	34.52173417797406	36.0	36.0	36.0	32.0	36.0
33	34.48177960738861	36.0	36.0	36.0	32.0	36.0
34	34.47279097806465	36.0	36.0	36.0	32.0	36.0
35	34.44042618386256	36.0	36.0	36.0	32.0	36.0
36	34.40939163219493	36.0	36.0	36.0	32.0	36.0
37	34.4019037722401	36.0	36.0	36.0	32.0	36.0
38	33.96488945731167	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	26.0
22	95.0
23	371.0
24	952.0
25	2452.0
26	4964.0
27	9909.0
28	17071.0
29	27209.0
30	41508.0
31	59976.0
32	86641.0
33	139030.0
34	340037.0
35	770986.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.394481783704094	18.194844983781906	12.150081839929491	26.26059139258451
2	15.975833150150208	19.925394509835268	36.74133876887618	27.357433571138333
3	18.181957461548198	23.900867954943614	28.489771720522505	29.427402862985687
4	12.040758686566846	15.620832572843796	35.42524576579409	36.91316297479526
5	14.326252472972161	36.80328797053083	32.85878912624981	16.011670430247197
6	35.11694100892137	35.405838024495964	15.964474393279632	13.512746573303039
7	30.887145121967468	30.405736667510425	19.967293268141912	18.7398249423802
8	28.810733322941605	32.53650094524758	18.62723938411691	20.025526347693905
9	27.489824603475952	13.774056236135815	18.162233457902836	40.5738857024854
10	16.131533457899103	26.241277898235758	30.756753696172765	26.870434947692367
11	38.25217314262837	20.9391759877482	21.355119050711878	19.453531818911554
12	25.1156553938878	23.71933092064025	27.859544226156334	23.305469459315614
13	29.740225655564185	18.98175224782944	24.97891723164933	26.29910486495704
14	23.836587331721322	19.55150110242934	24.598629124118222	32.013282441731114
15	25.462387508909362	26.856977278631522	22.08242574422307	25.598209468236043
16	26.111455273342525	25.609666739939918	23.22928531937145	25.04959266734611
17	24.302205524802993	25.77073466424199	24.64619012409824	25.28086968685678
18	25.31131139132578	24.290814865143915	26.084011110889072	24.313862632641232
19	25.80617227207024	24.621810115705124	25.28766411542535	24.28435349679929
20	26.012736222963834	23.713355048859935	25.065512946050905	25.208395782125326
21	27.39430652772264	23.695268140482327	24.3613894758158	24.549035855979238
22	26.207593911388607	23.76606100734798	24.99372181059652	25.0326232706669
23	24.43004879348532	23.813885326982962	25.74217722193542	26.013888657596297
24	25.047461081912832	24.800929904145267	24.996636091738107	25.154972922203793
25	25.1003011532551	24.243869522904234	25.135672172599914	25.520157151240753
26	24.815784390133423	25.148578165903956	25.613063954224202	24.422573489738415
27	25.586969076669845	24.406602856726057	24.843511549537077	25.162916517067018
28	24.595032073699567	24.30487000659459	25.827488126403015	25.272609793302824
29	24.562592007886867	24.46227426843322	25.8540663322742	25.121067391405717
30	24.569919332813758	24.695549649287585	25.985425284599962	24.749105733298695
31	25.238038142056844	24.54527287624148	24.688488772539852	25.528200209161824
32	25.034471733178794	24.401524083584793	24.643592254351432	25.920411928884977
33	24.531217734790804	24.202087621483717	25.29585739693451	25.97083724679096
34	25.348014628005032	24.39592867182244	25.494627738587695	24.761428961584834
35	25.915882309839265	24.097306875029144	25.50641807051551	24.48039274461608
36	24.61394989441991	24.99443789426004	25.034138672954843	25.357473538365205
37	25.805106479353597	24.759630436375506	24.633333999453782	24.801929084817118
38	24.76615841676492	24.575381520486534	25.29878832690527	25.359671735843275
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	53.5
2	75.0
3	75.0
4	325.0
5	575.0
6	575.0
7	895.0
8	1215.0
9	1398.5
10	1582.0
11	1582.0
12	2006.5
13	2431.0
14	3148.0
15	3865.0
16	3865.0
17	5827.5
18	7790.0
19	7790.0
20	9182.0
21	10574.0
22	10684.5
23	10795.0
24	10795.0
25	11891.0
26	12987.0
27	12987.0
28	17214.0
29	21441.0
30	26463.5
31	31486.0
32	31486.0
33	40159.0
34	48832.0
35	48832.0
36	54148.5
37	59465.0
38	68459.5
39	77454.0
40	77454.0
41	82528.0
42	87602.0
43	100335.5
44	113069.0
45	113069.0
46	116879.5
47	120690.0
48	120690.0
49	129310.5
50	137931.0
51	139729.0
52	141527.0
53	141527.0
54	136650.5
55	131774.0
56	131774.0
57	129005.5
58	126237.0
59	112984.0
60	99731.0
61	99731.0
62	94439.5
63	89148.0
64	74780.5
65	60413.0
66	60413.0
67	51042.0
68	41671.0
69	41671.0
70	33207.0
71	24743.0
72	19335.0
73	13927.0
74	13927.0
75	10509.0
76	7091.0
77	7091.0
78	6820.0
79	6549.0
80	5167.0
81	3785.0
82	3785.0
83	3392.0
84	2999.0
85	2999.0
86	1974.5
87	950.0
88	765.0
89	580.0
90	580.0
91	356.0
92	132.0
93	88.5
94	45.0
95	45.0
96	31.5
97	18.0
98	18.0
99	18.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008592953777902122
2	0.0
3	0.0
4	2.6644817915975563E-4
5	0.0
6	1.9983613436981676E-4
7	6.661204478993892E-4
8	0.002398033612437801
9	0.003996722687396335
10	9.991806718490836E-4
11	0.004196558821766152
12	1.9983613436981676E-4
13	2.6644817915975563E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.3322408957987781E-4
22	6.661204478993891E-5
23	3.330602239496946E-4
24	0.0
25	6.661204478993891E-5
26	0.0
27	6.661204478993891E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1501230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.64394511976063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.90594340609454	33.53976878018487
2	15.544101119558334	14.500763991133775
3	5.699278916704241	7.975105590388844
4	2.4884973273536932	4.642933310710267
5	1.3027955151169082	3.0383761254691675
6	0.7446327285347358	2.08395648744911
7	0.4870830790867241	1.5903633486779631
8	0.323126367896414	1.2057510856726337
9	0.23915971737185843	1.0039817458755385
>10	1.0499758449071988	8.821795632663544
>50	0.10083598390694215	3.2980734575818333
>100	0.09461362927290443	9.478818527083273
>500	0.013782812681864773	4.252414807695982
>1k	0.006029980548315838	4.173259621826744
>5k	1.4357096543609138E-4	0.39463748758665235
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5893	0.39254477994711007	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4055	0.2701118416232023	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2601	0.17325792849863111	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2291	0.15260819461375008	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2272	0.15134256576274122	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1882	0.12536386829466506	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1841	0.12263277445827754	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1819	0.12116730947289889	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1733	0.11543867362096415	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1727	0.1150390013522245	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1721	0.11463932908348487	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1708	0.11377337250121568	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1681	0.11197484729188732	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1648	0.10977664981381932	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1531	0.1019830405733965	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1522	0.10138353217028703	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	6.661204478993892E-5	0.0	0.0	0.0	0.0
11	6.661204478993892E-5	0.0	0.0	0.0	0.0
12	6.661204478993892E-5	0.0	0.0	0.0	6.661204478993892E-5
13	6.661204478993892E-5	0.0	0.0	0.0	6.661204478993892E-5
14	6.661204478993892E-5	0.0	0.0	0.0	6.661204478993892E-5
15	1.9983613436981676E-4	0.0	0.0	0.0	6.661204478993892E-5
16	1.9983613436981676E-4	0.0	0.0	6.661204478993892E-5	6.661204478993892E-5
17	2.664481791597557E-4	0.0	0.0	6.661204478993892E-5	6.661204478993892E-5
18	2.664481791597557E-4	0.0	0.0	6.661204478993892E-5	6.661204478993892E-5
19	3.330602239496946E-4	0.0	0.0	1.3322408957987784E-4	6.661204478993892E-5
20	3.330602239496946E-4	0.0	0.0	3.330602239496946E-4	6.661204478993892E-5
21	3.996722687396335E-4	0.0	0.0	3.330602239496946E-4	6.661204478993892E-5
22	3.996722687396335E-4	0.0	0.0	3.996722687396335E-4	6.661204478993892E-5
23	4.662843135295724E-4	0.0	0.0	5.995084031094502E-4	6.661204478993892E-5
24	4.662843135295724E-4	0.0	0.0	0.001665301119748473	6.661204478993892E-5
25	5.328963583195114E-4	0.0	0.0	0.0019317492989082286	6.661204478993892E-5
26	5.328963583195114E-4	0.0	0.0	0.00246464565722774	1.9983613436981676E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATAC	65	9.4343395E-6	19.692944	3
TACCGTC	340	0.0	17.884123	7
ACCGTCG	365	0.0	17.535397	8
CGTCGTA	375	0.0	16.64109	10
TCTAGCG	415	0.0	16.577744	28
ATACCGT	370	0.0	16.43351	6
TAGCGGC	430	0.0	16.37153	30
AACCGCG	235	0.0	16.342041	7
CTAGCGG	425	0.0	16.18768	29
TACGGAC	70	3.6973695E-4	15.999983	13
CGCAAGA	505	0.0	15.842095	2
CCGTCGT	395	0.0	15.798504	9
GTCGTAG	390	0.0	15.590765	11
AAGACGG	515	0.0	15.534999	5
ATTACAC	125	5.1379175E-8	15.360496	3
CGATAAC	220	0.0	15.273729	10
CCGATAA	235	0.0	14.979706	9
GCGCAAG	535	0.0	14.954252	1
ACGAACG	225	0.0	14.93282	15
CCCCTAT	65	0.004160554	14.7702	1
>>END_MODULE