FastQCFastQC Report
Thu 2 Feb 2017
SRR4062912_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062912_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568312
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41370.26378679752498224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27490.17528399961232202No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20890.13320053662791587No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19770.12605910048510757No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18490.11789745917904089No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17660.11260514489463833No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16630.1060375741561628No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16390.1045072664112753No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16380.10444350358857166No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16370.10437974076586802No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16340.10418845229775708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15990.10195675350312948No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15840.1010003111625748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT3300.020.84815229
TCTAGCG2900.020.41281528
CATCGTT3450.019.94107628
CGGCATC3900.017.64018225
TAGCGGC3350.017.19375630
TAGGACG753.2397777E-517.0636734
TCGTTTA3850.016.62311230
GCATCGT4050.016.59179927
GGCATCG4150.016.5775226
CTAGCGG3400.016.47032529
CGTTTAT4150.015.80697731
CAAGACG4900.015.3442484
TATACTG1807.2759576E-1215.1084595
CGCAAGA4750.014.8288552
GCGCAAG4800.014.6846951
AGGCCCG6050.014.54475910
CCGATAA2650.014.4898749
TAGCGTA3000.014.3988527
TAGACAG3000.014.3974745
ATAGCGT3150.014.2197286
Produced by FastQC (version 0.11.5)