##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062912_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1568312
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2505636633527	32.0	32.0	32.0	32.0	32.0
2	30.867569080642117	32.0	32.0	32.0	32.0	32.0
3	30.91402029698172	32.0	32.0	32.0	32.0	32.0
4	30.98369775911936	32.0	32.0	32.0	32.0	32.0
5	30.898244737016615	32.0	32.0	32.0	32.0	32.0
6	34.5611746897301	36.0	36.0	36.0	32.0	36.0
7	34.49682461142936	36.0	36.0	36.0	32.0	36.0
8	34.468230173587905	36.0	36.0	36.0	32.0	36.0
9	34.60056162294237	36.0	36.0	36.0	32.0	36.0
10	34.326150026270284	36.0	36.0	36.0	32.0	36.0
11	34.60194463856681	36.0	36.0	36.0	32.0	36.0
12	34.41996809308352	36.0	36.0	36.0	32.0	36.0
13	34.504377317778605	36.0	36.0	36.0	32.0	36.0
14	34.38466963206301	36.0	36.0	36.0	32.0	36.0
15	34.342328567274876	36.0	36.0	36.0	32.0	36.0
16	34.355100898290644	36.0	36.0	36.0	32.0	36.0
17	34.29204265477787	36.0	36.0	36.0	32.0	36.0
18	34.309266268446585	36.0	36.0	36.0	32.0	36.0
19	34.29432663908712	36.0	36.0	36.0	32.0	36.0
20	34.27827753661261	36.0	36.0	36.0	32.0	36.0
21	34.26188921592132	36.0	36.0	36.0	32.0	36.0
22	34.24138691790919	36.0	36.0	36.0	32.0	36.0
23	34.17924877192804	36.0	36.0	36.0	32.0	36.0
24	34.16429383949112	36.0	36.0	36.0	32.0	36.0
25	34.153943220481636	36.0	36.0	36.0	32.0	36.0
26	34.0984727528706	36.0	36.0	36.0	32.0	36.0
27	34.10690729905784	36.0	36.0	36.0	32.0	36.0
28	34.094466534720134	36.0	36.0	36.0	32.0	36.0
29	34.0579412769908	36.0	36.0	36.0	32.0	36.0
30	34.02396972031075	36.0	36.0	36.0	32.0	36.0
31	34.04027514933253	36.0	36.0	36.0	32.0	36.0
32	34.004497191885285	36.0	36.0	36.0	32.0	36.0
33	33.95758815847867	36.0	36.0	36.0	32.0	36.0
34	33.96192849382011	36.0	36.0	36.0	32.0	36.0
35	33.925595162187115	36.0	36.0	36.0	32.0	36.0
36	33.887701554282565	36.0	36.0	36.0	32.0	36.0
37	33.882213488132464	36.0	36.0	36.0	32.0	36.0
38	33.29998750248675	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	4.0
10	10.0
11	12.0
12	11.0
13	3.0
14	142.0
15	331.0
16	453.0
17	523.0
18	673.0
19	915.0
20	1283.0
21	1870.0
22	2978.0
23	4572.0
24	6828.0
25	10440.0
26	15258.0
27	21749.0
28	30401.0
29	40959.0
30	55043.0
31	73490.0
32	100601.0
33	150436.0
34	336297.0
35	713023.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.13159423620821	16.735745341020092	11.139159691763549	25.99350073100815
2	17.039714738328303	19.12022625961543	37.327158363228854	26.512900638827407
3	19.291751082984028	23.11186445433739	28.125316860114747	29.47106760256383
4	12.576493671039435	14.788872047935714	35.67727944284997	36.95735483817488
5	14.811102396847561	36.41308159738827	33.432994752313	15.342821253451167
6	35.067215622411624	35.15705749688678	16.43589823702059	13.339828643681004
7	31.047776207795387	30.19922056325527	20.202485219777696	18.550518009171643
8	28.815659178974524	32.56058922137056	18.9518378735796	19.671913726075317
9	27.730823453796415	13.807228039882446	18.12627486037509	40.335673645946045
10	15.913677505332192	26.2927172966993	31.556489330838073	26.237115867130438
11	38.34559618233142	20.873857909936127	21.524504124563933	19.256041783168516
12	24.965296309261692	23.508857172553064	28.392050639013387	23.133795879171856
13	29.91711567391902	19.03979939914125	24.888638336216886	26.154446590722845
14	23.738433970806735	19.318249934637187	24.97924332183373	31.964072772722346
15	25.479574018115326	26.899322301547112	22.14267765508427	25.47842602525329
16	26.02079403523405	25.54022531122819	23.501188030165636	24.93779262337212
17	24.306218997169644	25.648500448341267	24.877901941451604	25.167378613037485
18	25.354749559851015	24.529227104122576	25.816228473191565	24.299794862834847
19	25.802987617698186	24.665700807164363	25.304456174762752	24.226855400374696
20	26.07546748712717	23.789060880932265	24.9250585853433	25.210413046597267
21	27.316268463702613	23.871071003155187	24.308448092477292	24.50421244066491
22	26.113040068103345	23.949547412487526	24.89617110007939	25.04124141932974
23	24.52426759752347	24.014626049238565	25.363212068148616	26.097894285089353
24	24.918181029732875	24.69415137848703	25.15949278615595	25.228174805624143
25	25.137097818233066	24.36696489441284	24.95266386529434	25.543273422059755
26	24.907175810481768	25.24919994540879	25.383573402856346	24.460050841253096
27	25.69058201598041	24.53426605533839	24.591211412028034	25.18394051665317
28	24.71410516542812	24.49851233935752	25.52444341852045	25.262939076693915
29	24.638884478106686	24.833830423173634	25.58772344331805	24.939561655401636
30	24.648194920641803	25.065215214991817	25.64056293913863	24.64602692522775
31	25.252165196420396	24.88078825775498	24.488368326342382	25.378678219482243
32	24.985859683422703	24.72887895825243	24.417186207723322	25.868075150601545
33	24.61280251397788	24.375012753540382	25.07556472676815	25.936620005713586
34	25.399601944490325	24.72159390558011	25.04127546117237	24.837528688757196
35	25.954774862792767	24.35803084819134	25.272022988828276	24.41517130018762
36	24.62988153020352	25.11933391111035	24.86571148519941	25.38507307348673
37	25.774239853177406	24.84537522215654	24.679829939846353	24.7005549848197
38	24.69847942059076	24.7715559954037	25.248211664449716	25.281752919555828
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	82.0
1	124.0
2	166.0
3	166.0
4	428.5
5	691.0
6	691.0
7	929.5
8	1168.0
9	1175.5
10	1183.0
11	1183.0
12	1542.0
13	1901.0
14	2540.0
15	3179.0
16	3179.0
17	4706.5
18	6234.0
19	6234.0
20	7642.5
21	9051.0
22	9676.5
23	10302.0
24	10302.0
25	12104.0
26	13906.0
27	13906.0
28	18132.5
29	22359.0
30	28040.5
31	33722.0
32	33722.0
33	42053.5
34	50385.0
35	50385.0
36	56915.5
37	63446.0
38	73144.5
39	82843.0
40	82843.0
41	89731.5
42	96620.0
43	109097.0
44	121574.0
45	121574.0
46	125741.5
47	129909.0
48	129909.0
49	137086.5
50	144264.0
51	146613.0
52	148962.0
53	148962.0
54	142654.0
55	136346.0
56	136346.0
57	131656.0
58	126966.0
59	114396.5
60	101827.0
61	101827.0
62	96428.0
63	91029.0
64	76298.0
65	61567.0
66	61567.0
67	51820.0
68	42073.0
69	42073.0
70	33962.5
71	25852.0
72	20385.5
73	14919.0
74	14919.0
75	11425.5
76	7932.0
77	7932.0
78	7260.5
79	6589.0
80	5173.5
81	3758.0
82	3758.0
83	3638.5
84	3519.0
85	3519.0
86	2430.5
87	1342.0
88	1118.0
89	894.0
90	894.0
91	664.0
92	434.0
93	378.5
94	323.0
95	323.0
96	282.0
97	241.0
98	241.0
99	497.5
100	754.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1263779145986258
2	0.05796040583761395
3	0.01217869913639633
4	0.0028693270216640565
5	1.275256454072914E-4
6	6.37628227036457E-5
7	0.0
8	3.188141135182285E-4
9	1.9128846811093713E-4
10	4.463397589255199E-4
11	0.0015940705675911427
12	0.0022316987946275997
13	0.014219109462912993
14	0.00905432082391769
15	0.022827090527905162
16	0.010839679859619769
17	0.019766475038130168
18	0.0059299425114390505
19	0.006758859206586445
20	0.005547365575217176
21	0.006376282270364571
22	0.00682262202929009
23	0.0096281862282505
24	0.013772769703987471
25	0.017279724952687984
26	0.018746269874871838
27	0.00905432082391769
28	0.0049735001708843644
29	0.011796122200174454
30	0.002741801376256765
31	0.005993705334142696
32	0.0064400450930682165
33	0.007651538724437484
34	0.012051173490989038
35	0.015685654385096844
36	0.01485673768994945
37	0.010265814455286959
38	0.005993705334142696
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1568312.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.004367007472744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.69945018414485	40.88100890008459
2	14.308105674161231	15.454003800182125
3	4.85890636024298	7.872064870005163
4	2.030224639223147	4.385639860968831
5	0.9871910610062395	2.6656314182538687
6	0.5489161417852305	1.778632126637732
7	0.32413529103151995	1.2253304851858133
8	0.22938522692799992	0.99102431848897
9	0.14637773880342578	0.711453341525777
>10	0.725825238191568	6.910717050724887
>50	0.05954699183252795	2.2834705595631206
>100	0.06772719128219411	7.854746401145113
>500	0.009827380778869384	3.495152617540767
>1k	0.004380880588170689	3.4911242496933976
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4137	0.26378679752498224	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2749	0.17528399961232202	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2089	0.13320053662791587	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1977	0.12605910048510757	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1849	0.11789745917904089	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1766	0.11260514489463833	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1663	0.1060375741561628	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1639	0.1045072664112753	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1638	0.10444350358857166	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1637	0.10437974076586802	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1634	0.10418845229775708	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1599	0.10195675350312948	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1584	0.1010003111625748	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.275256454072914E-4
2	0.0	0.0	0.0	0.0	1.275256454072914E-4
3	0.0	0.0	0.0	0.0	1.275256454072914E-4
4	0.0	0.0	0.0	0.0	1.275256454072914E-4
5	0.0	0.0	0.0	0.0	1.275256454072914E-4
6	0.0	0.0	0.0	0.0	1.275256454072914E-4
7	0.0	0.0	0.0	0.0	1.275256454072914E-4
8	0.0	0.0	0.0	6.37628227036457E-5	1.275256454072914E-4
9	0.0	0.0	0.0	6.37628227036457E-5	1.275256454072914E-4
10	0.0	0.0	0.0	6.37628227036457E-5	1.275256454072914E-4
11	0.0	0.0	0.0	6.37628227036457E-5	1.275256454072914E-4
12	0.0	0.0	0.0	6.37628227036457E-5	3.188141135182285E-4
13	0.0	0.0	0.0	6.37628227036457E-5	3.188141135182285E-4
14	0.0	0.0	0.0	1.275256454072914E-4	3.188141135182285E-4
15	0.0	0.0	0.0	1.275256454072914E-4	3.825769362218742E-4
16	0.0	0.0	0.0	1.912884681109371E-4	5.101025816291656E-4
17	0.0	0.0	0.0	1.912884681109371E-4	5.101025816291656E-4
18	0.0	0.0	0.0	1.912884681109371E-4	5.101025816291656E-4
19	0.0	0.0	0.0	1.912884681109371E-4	5.101025816291656E-4
20	0.0	0.0	0.0	2.550512908145828E-4	5.101025816291656E-4
21	0.0	0.0	0.0	3.825769362218742E-4	5.101025816291656E-4
22	0.0	0.0	0.0	3.825769362218742E-4	5.101025816291656E-4
23	0.0	0.0	0.0	0.001083967985961977	5.101025816291656E-4
24	0.0	0.0	0.0	0.0015940705675911427	5.101025816291656E-4
25	0.0	0.0	0.0	0.0018491218584057254	5.101025816291656E-4
26	0.0	0.0	0.0	0.002359224440034891	5.101025816291656E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	330	0.0	20.848152	29
TCTAGCG	290	0.0	20.412815	28
CATCGTT	345	0.0	19.941076	28
CGGCATC	390	0.0	17.640182	25
TAGCGGC	335	0.0	17.193756	30
TAGGACG	75	3.2397777E-5	17.063673	4
TCGTTTA	385	0.0	16.623112	30
GCATCGT	405	0.0	16.591799	27
GGCATCG	415	0.0	16.57752	26
CTAGCGG	340	0.0	16.470325	29
CGTTTAT	415	0.0	15.806977	31
CAAGACG	490	0.0	15.344248	4
TATACTG	180	7.2759576E-12	15.108459	5
CGCAAGA	475	0.0	14.828855	2
GCGCAAG	480	0.0	14.684695	1
AGGCCCG	605	0.0	14.544759	10
CCGATAA	265	0.0	14.489874	9
TAGCGTA	300	0.0	14.398852	7
TAGACAG	300	0.0	14.397474	5
ATAGCGT	315	0.0	14.219728	6
>>END_MODULE