FastQCFastQC Report
Thu 2 Feb 2017
SRR4062912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568312
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT44520.28387208667663066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT32990.21035355209932716No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21740.13862037655772574No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20500.1307137865424737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19870.126696728712144No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18590.11853508740607736No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18300.11668596554767165No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18060.11515565780278415No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17290.11024592045460342No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16680.10635638826968104No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16550.10552747157453364No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15970.1018292278577222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG4050.017.77676829
ATCGTTT3650.017.09492129
TCTAGCG4350.016.9185828
CGTCGTA4200.016.76255610
TAGCGGC4300.016.74323530
TCGTTTA3800.016.4201230
CATCGTT3800.016.4201228
TACCGTC4400.016.3658377
ATACCGT4450.016.1819536
GTCGTAG4600.015.65228311
ACCGTCG4500.015.6465498
CCGTCGT4500.015.646059
CTAGATA3200.015.5010983
GTTTTCG4650.015.13892628
CGCCCCT2550.015.060855
GGCATCG4250.015.05796826
GCATCGT4300.014.88287627
CGTTTAT4200.014.85629931
AGCGGCG5000.014.71916531
TTAATAC1851.0913936E-1114.7037433