FastQCFastQC Report
Thu 2 Feb 2017
SRR4062911_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062911_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265528
Sequences flagged as poor quality0
Sequence length38
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38791.4608628845168872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27031.017971739326926No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17990.6775180018679762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16430.618767135669308No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13880.5227320659214847No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA13150.4952396734054412No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.44891687505649125No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA7490.28207947937693956No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6350.23914615407791268No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA6190.2331204242113826No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG5980.22521165376156188No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA5950.22408182941158747No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT5840.21993914012834806No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5540.20864089662860413No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5330.2007321261787834No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.1931999638456208No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA5060.1905637070290139No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4860.18303154469585128No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT4730.1781356391792956No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.170603476846133No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA4500.1694736524961586No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA4440.16721400379620982No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA4150.15629236841312402No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG4070.153279503479859No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA4040.1521496791298846No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.14235786809677323No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG3680.13859178693019192No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA3570.13444909764695248No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3460.13030640836371304No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC3440.1295531921303968No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT3290.12390407038052485No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG3120.11750173239733663No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA2930.11034617818083214No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA2920.10996957006417403No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA2850.10733331324756712No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT2840.10695670513090898No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGTATG2800.10545027266427645No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC2800.10545027266427645No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC2770.10432044831430207No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA2760.10394384019764395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGTT252.8897967E-431.99868227
ATAATAC200.003749116331.998683
GTCAATA200.003749116331.9986813
CGCCGGG356.4141335E-527.4274418
AATTTGT308.4017613E-426.6655727
AATACAT308.4110117E-426.6605455
TGACCCG451.2338027E-524.88786313
CCCATGA451.2338027E-524.8878639
GCCGGGC401.5894987E-423.9990119
TCTAGCG752.4720066E-923.465728
GTTATAT552.1906199E-623.2937091
TCAATAA350.002062877222.85620114
TGGGATA350.00206513822.8518966
GACCCGC502.7880425E-522.39907614
CCATGAC502.7880425E-522.39907610
ATGACCC502.7880425E-522.39907612
ACCCGCC502.7880425E-522.39907615
CCCGCCG502.7880425E-522.39907616
CTAGCGG805.2095857E-921.99909229
TAGGACC1700.021.6461684
Produced by FastQC (version 0.11.5)