##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062911_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	265528
Sequences flagged as poor quality	0
Sequence length	38
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.853657618028983	32.0	32.0	32.0	32.0	32.0
2	30.652266427646047	32.0	32.0	32.0	32.0	32.0
3	30.791965442439217	32.0	32.0	32.0	32.0	32.0
4	30.759106384260793	32.0	32.0	32.0	32.0	32.0
5	30.850802928504717	32.0	32.0	32.0	32.0	32.0
6	34.420411406706634	36.0	36.0	36.0	32.0	36.0
7	34.18624024584978	36.0	36.0	36.0	32.0	36.0
8	34.1573431050586	36.0	36.0	36.0	32.0	36.0
9	34.16953390979482	36.0	36.0	36.0	32.0	36.0
10	34.04941851706788	36.0	36.0	36.0	32.0	36.0
11	34.35378189870748	36.0	36.0	36.0	32.0	36.0
12	34.183765930523336	36.0	36.0	36.0	32.0	36.0
13	34.243853755536136	36.0	36.0	36.0	32.0	36.0
14	34.1764446687355	36.0	36.0	36.0	32.0	36.0
15	34.186598023560606	36.0	36.0	36.0	32.0	36.0
16	34.17296857581874	36.0	36.0	36.0	32.0	36.0
17	34.109163628694525	36.0	36.0	36.0	32.0	36.0
18	34.15643924557862	36.0	36.0	36.0	32.0	36.0
19	34.082883914314124	36.0	36.0	36.0	32.0	36.0
20	33.923326353529575	36.0	36.0	36.0	32.0	36.0
21	33.85285920882167	36.0	36.0	36.0	32.0	36.0
22	33.86253427133862	36.0	36.0	36.0	32.0	36.0
23	33.864518996113404	36.0	36.0	36.0	32.0	36.0
24	33.83813383146033	36.0	36.0	36.0	32.0	36.0
25	33.81524359012985	36.0	36.0	36.0	32.0	36.0
26	33.80643849236239	36.0	36.0	36.0	32.0	36.0
27	33.802088668614985	36.0	36.0	36.0	32.0	36.0
28	33.73132776957609	36.0	36.0	36.0	27.0	36.0
29	33.67926922961043	36.0	36.0	36.0	27.0	36.0
30	33.57407881654665	36.0	36.0	36.0	27.0	36.0
31	33.5955982043325	36.0	36.0	36.0	27.0	36.0
32	33.50047829230816	36.0	36.0	36.0	21.0	36.0
33	33.42079178090446	36.0	36.0	36.0	21.0	36.0
34	33.310833509083785	36.0	36.0	36.0	21.0	36.0
35	33.222236449639965	36.0	36.0	36.0	21.0	36.0
36	33.142915248109425	36.0	36.0	36.0	14.0	36.0
37	33.122518152511226	36.0	36.0	36.0	14.0	36.0
38	32.36282049350728	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	1.0
14	199.0
15	298.0
16	281.0
17	334.0
18	394.0
19	498.0
20	626.0
21	793.0
22	1024.0
23	1389.0
24	1732.0
25	2269.0
26	3045.0
27	4285.0
28	5927.0
29	7837.0
30	10518.0
31	13460.0
32	17830.0
33	25415.0
34	54447.0
35	112923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.18843924532287	18.50791747274421	12.918358958114137	21.38528432381879
2	12.530053285700287	22.70784814705949	44.21130380386039	20.550794763379837
3	19.301232410281134	30.311944436074366	29.905535299966857	20.481287853677646
4	9.93179447200033	18.440725968944076	38.60523273112108	33.02224682793452
5	10.162770028019644	41.97184477719863	34.23254797987406	13.632837214907656
6	26.938880038564818	39.94245406305197	20.102663759241057	13.016002139142158
7	24.439230514296046	34.44420174143593	22.217995842246392	18.898571902021633
8	25.95583140007833	36.26397216112802	20.759392606429454	17.020803832364194
9	28.073498840047	14.406390286523454	20.14815763309331	37.37195324033624
10	15.641347207628604	29.6338978710263	32.69347373336798	22.031281187977118
11	34.147572347509076	23.34252270981154	24.68590409906449	17.82400084361489
12	24.120710888158268	26.811962625269746	32.62493079799794	16.442395688574045
13	30.759755913816484	22.57797197529004	27.291321380141632	19.370950730751847
14	22.310522886402243	23.997860696704794	28.177788156244464	25.513828260648495
15	23.234534043563105	31.140611932764244	27.734748770031718	17.890105253640932
16	19.925122128520258	28.104437304848606	31.760571602906225	20.20986896372491
17	18.458721955188572	28.05987915680986	30.88884535989272	22.59255352810885
18	19.47257614390039	26.65742683142699	35.75251302176509	18.117484002907535
19	22.370740305522915	26.726099008707706	30.52140769532074	20.381752990448643
20	22.389065901370152	26.555261790161115	32.797399799636935	18.258272508831794
21	24.751990478844807	25.102254495054876	28.016978389086827	22.12877663701349
22	22.013732217982955	28.715627083278406	29.55289314406023	19.717747554678407
23	21.192843691148777	28.132580037664784	30.067796610169488	20.60677966101695
24	21.2066082082806	29.434852046043513	30.594603145904898	18.76393659977099
25	22.07971251963432	27.420624606842726	31.19794786028273	19.30171501324022
26	17.677127240811682	29.597360183521488	33.84524979941463	18.8802627762522
27	20.016195551872844	29.04766388580253	32.47773111617484	18.45840944614979
28	18.54029278507376	29.95567172217431	31.029041236220383	20.47499425653155
29	18.567608286252355	27.817702448210923	31.443691148775894	22.170998116760828
30	19.157213660535394	29.36871996505024	31.507133065184316	19.96693330923005
31	20.1205962774091	28.555933020481028	30.546035237313284	20.777435464796586
32	18.30637932463072	31.098171847812168	30.732089456676487	19.86335937088063
33	18.69496440812022	29.948401190162325	31.590900531053446	19.765733870664004
34	18.714994368966416	30.725857178908672	32.64153857163842	17.91760988048649
35	19.443879434387267	30.888346479233995	31.67182709185557	17.99594699452317
36	18.232223168076658	31.90827388261528	30.44830989204704	19.411193057261023
37	19.25416476774099	31.69215935156553	29.483504770226855	19.570171110466628
38	19.143999939739523	33.04458521961177	28.45763311915756	19.353781721491146
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	644.0
1	515.5
2	387.0
3	387.0
4	835.0
5	1283.0
6	1283.0
7	1490.0
8	1697.0
9	1593.5
10	1490.0
11	1490.0
12	1871.0
13	2252.0
14	2471.0
15	2690.0
16	2690.0
17	3856.0
18	5022.0
19	5022.0
20	5546.5
21	6071.0
22	5725.0
23	5379.0
24	5379.0
25	6402.0
26	7425.0
27	7425.0
28	9983.5
29	12542.0
30	15301.5
31	18061.0
32	18061.0
33	19372.5
34	20684.0
35	20684.0
36	20634.5
37	20585.0
38	21393.5
39	22202.0
40	22202.0
41	20850.5
42	19499.0
43	19471.5
44	19444.0
45	19444.0
46	20741.5
47	22039.0
48	22039.0
49	20152.5
50	18266.0
51	15194.0
52	12122.0
53	12122.0
54	10899.0
55	9676.0
56	9676.0
57	9239.5
58	8803.0
59	7808.5
60	6814.0
61	6814.0
62	6655.5
63	6497.0
64	5645.0
65	4793.0
66	4793.0
67	4173.0
68	3553.0
69	3553.0
70	2887.5
71	2222.0
72	1765.5
73	1309.0
74	1309.0
75	966.5
76	624.0
77	624.0
78	574.0
79	524.0
80	398.0
81	272.0
82	272.0
83	277.0
84	282.0
85	282.0
86	188.0
87	94.0
88	80.5
89	67.0
90	67.0
91	49.5
92	32.0
93	28.5
94	25.0
95	25.0
96	26.0
97	27.0
98	27.0
99	78.5
100	130.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13369588141363623
2	0.06251694736524963
3	0.012051459733060167
4	0.002636256816606912
5	0.0
6	3.766081166581302E-4
7	0.0
8	0.0
9	0.0
10	3.766081166581302E-4
11	0.0015064324666325209
12	3.766081166581302E-4
13	0.01807718959959025
14	0.007908770449820733
15	0.027869000632701632
16	0.010921635383085777
17	0.022596486999487814
18	0.0041426892832394325
19	0.006025729866530083
20	0.003766081166581302
21	0.005272513633213824
22	0.007908770449820733
23	0.010545027266427648
24	0.015064324666325207
25	0.01845379771624838
26	0.020713446416197163
27	0.008661986683136995
28	0.003389473049923172
29	0.010545027266427648
30	0.0015064324666325209
31	0.005272513633213824
32	0.005272513633213824
33	0.006778946099846344
34	0.013181284083034556
35	0.01732397336627399
36	0.015817540899641467
37	0.010168419149769516
38	0.005272513633213824
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	265528.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.8998225737609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.80129711548653	29.097571860795995
2	13.128356221148291	10.213814553755974
3	4.355426736526299	5.082759818516626
4	1.9915370462196227	3.098817509880608
5	1.140490696615191	2.2182442872677957
6	0.7280302923878338	1.6992149521326005
7	0.48535311658888847	1.3216105084650756
8	0.38848219444740817	1.208951074965557
9	0.29960066160842286	1.0488971321594125
>10	2.1950741787378276	17.17743868540998
>50	0.2966046547215591	8.05591844804911
>100	0.1727697147031304	12.08290647102626
>500	0.009986688711163608	2.2865957460200157
>1k	0.006990682097814525	5.4072589515549785
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3879	1.4608628845168872	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2703	1.017971739326926	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1799	0.6775180018679762	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1643	0.618767135669308	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1388	0.5227320659214847	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1315	0.4952396734054412	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1192	0.44891687505649125	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	749	0.28207947937693956	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	635	0.23914615407791268	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	619	0.2331204242113826	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	598	0.22521165376156188	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	595	0.22408182941158747	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	584	0.21993914012834806	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	554	0.20864089662860413	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	533	0.2007321261787834	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	513	0.1931999638456208	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	506	0.1905637070290139	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	486	0.18303154469585128	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	473	0.1781356391792956	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	453	0.170603476846133	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	450	0.1694736524961586	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	444	0.16721400379620982	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	415	0.15629236841312402	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	407	0.153279503479859	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	404	0.1521496791298846	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	378	0.14235786809677323	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	368	0.13859178693019192	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	357	0.13444909764695248	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	346	0.13030640836371304	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	344	0.1295531921303968	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	329	0.12390407038052485	No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG	312	0.11750173239733663	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA	293	0.11034617818083214	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	292	0.10996957006417403	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	285	0.10733331324756712	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	284	0.10695670513090898	No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGTATG	280	0.10545027266427645	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	280	0.10545027266427645	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	277	0.10432044831430207	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	276	0.10394384019764395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	3.766081166581302E-4	0.0	0.0	0.0	0.0
11	3.766081166581302E-4	0.0	0.0	0.0	0.0
12	3.766081166581302E-4	0.0	0.0	3.766081166581302E-4	0.0
13	3.766081166581302E-4	0.0	0.0	3.766081166581302E-4	0.0
14	3.766081166581302E-4	0.0	0.0	3.766081166581302E-4	0.0
15	3.766081166581302E-4	0.0	0.0	3.766081166581302E-4	0.0
16	3.766081166581302E-4	0.0	0.0	7.532162333162604E-4	0.0
17	3.766081166581302E-4	0.0	0.0	7.532162333162604E-4	0.0
18	3.766081166581302E-4	0.0	0.0	7.532162333162604E-4	0.0
19	3.766081166581302E-4	0.0	0.0	0.0018830405832906511	0.0
20	3.766081166581302E-4	0.0	0.0	0.0018830405832906511	0.0
21	3.766081166581302E-4	0.0	0.0	0.002259648699948781	0.0
22	3.766081166581302E-4	0.0	0.0	0.003389473049923172	0.0
23	3.766081166581302E-4	0.0	0.0	0.0075321623331626045	0.0
24	3.766081166581302E-4	0.0	0.0	0.013557892199692688	0.0
25	3.766081166581302E-4	0.0	0.0	0.01770058148293212	0.0
26	3.766081166581302E-4	0.0	0.0	0.022973095116145942	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCACGTT	25	2.8897967E-4	31.998682	27
ATAATAC	20	0.0037491163	31.99868	3
GTCAATA	20	0.0037491163	31.99868	13
CGCCGGG	35	6.4141335E-5	27.42744	18
AATTTGT	30	8.4017613E-4	26.66557	27
AATACAT	30	8.4110117E-4	26.660545	5
TGACCCG	45	1.2338027E-5	24.887863	13
CCCATGA	45	1.2338027E-5	24.887863	9
GCCGGGC	40	1.5894987E-4	23.99901	19
TCTAGCG	75	2.4720066E-9	23.4657	28
GTTATAT	55	2.1906199E-6	23.293709	1
TCAATAA	35	0.0020628772	22.856201	14
TGGGATA	35	0.002065138	22.851896	6
GACCCGC	50	2.7880425E-5	22.399076	14
CCATGAC	50	2.7880425E-5	22.399076	10
ATGACCC	50	2.7880425E-5	22.399076	12
ACCCGCC	50	2.7880425E-5	22.399076	15
CCCGCCG	50	2.7880425E-5	22.399076	16
CTAGCGG	80	5.2095857E-9	21.999092	29
TAGGACC	170	0.0	21.646168	4
>>END_MODULE