FastQCFastQC Report
Thu 2 Feb 2017
SRR4062911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265528
Sequences flagged as poor quality0
Sequence length38
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT48001.8077189599590249No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34001.2804675966376426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22840.8601729384471695No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC17480.6583109879184116No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17340.6530384742851978No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15070.5675484318038023No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA11820.44515079388990997No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6720.25308065439426347No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA6660.2508210056943147No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.23952276219457083No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG6250.23538007291133137No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.22935434304480132No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA6060.2282245186948269No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT6000.2259648699948781No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.21880931577837365No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA5630.21203036967852731No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA5570.20977072097857855No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5430.20449820734536472No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5360.2018619505287578No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA5320.2003555180621253No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA5070.19094031514567203No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT4680.17625259859600492No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA4250.16005844957970533No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA4230.15930523334638907No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC3910.14725377361332892No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG3720.14009821939682443No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG3630.13670874634690128No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC3630.13670874634690128No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3600.13557892199692687No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA3590.13520231388026874No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT3330.12541050284715735No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA3330.12541050284715735No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA3320.12503389473049925No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC3230.12164442168057606No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA3210.1208912054472598No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA3170.11938477298062727No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGTATG2960.11147600253080653No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG2780.1046970564309602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG200.003749117331.99868427
TATAGGA401.3673707E-731.9986842
CTAGCGG200.003749117331.99868429
TCTAGCA451.2338034E-524.8878633
TGGATAC451.2338034E-524.88786324
ATTTAGA1100.024.7262551
TTTAGAA1250.024.3189982
GTATAAA350.002062877222.85620327
ATAGGAC854.2564352E-1022.5873053
TTAGAAA1350.022.5175913
CAAGTAT502.7880453E-522.39907613
CACAAAT502.7880453E-522.39907624
CCGTTCT502.7880453E-522.3990769
TTAATAC453.5278665E-421.3324553
TAGAAAT1500.021.3324554
CCGAGCC756.358641E-821.33245514
GGGTTCC453.5278665E-421.3324557
TACACAA453.5278665E-421.33245522
CAAGTTA604.6996993E-621.33245322
GCAGGCC604.6996993E-621.3324538
Produced by FastQC (version 0.11.5)