FastQCFastQC Report
Thu 2 Feb 2017
SRR4062910_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062910_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308730
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24980.1908720668128644No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22340.1706998387749956No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22120.16901881977183988No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21980.16794908040619533No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20860.15939116548103888No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19780.151138890374638No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19620.14991633109961566No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19580.14961069128086008No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19490.14892300168866No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19380.14808249218708214No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19160.14640147318392638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17210.1315015320195915No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT16710.12768103428514666No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA16640.1271461646023244No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16550.12645847501012433No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC16280.12439540623352409No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16260.12424258632414631No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16020.12240874741161278No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15920.12164464786472381No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15660.1196579890428125No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15650.1195815790881236No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT14850.11346878271301183No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC14430.11025956461607818No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT14390.1099539247973226No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG14300.10926623520512253No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG14240.10880777547698914No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG13900.10620983701756664No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT13880.10605701710818885No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT13720.1048344578331665No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC13160.10055550037058827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATG400.004483712420.0000729
AAACGCT3300.017.93945929
TTGGCGT450.00886047417.7744454
CAAGACG5000.017.276764
ACGCTTC3250.017.23017331
AAGACGG5200.017.226885
AACCGCG3100.017.0303677
TAAACGC3400.016.94123628
TTAGACT854.993897E-616.9380024
CGTCTTA1354.4383341E-1016.59265315
CGCTTCG3400.016.47001632
CACGTTT1850.016.43249128
ACGTTTT2050.016.39030329
TCTAGCG5100.016.31378428
CGCAAGA5300.016.0049512
TAGCGGC5200.016.00005730
AACGCTT3700.016.00005730
TCACGTT1703.6379788E-1215.99944527
TAGGTTG600.002445829615.9963895
TATACAG1900.015.9963885
Produced by FastQC (version 0.11.5)