FastQCFastQC Report
Thu 2 Feb 2017
SRR4062910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308730
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28840.22036630932277856No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24420.18659310935028617No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22780.17406187678130708No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22360.17085265868437338No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21340.1630588433061059No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21240.16229474375921696No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20840.15923834557166106No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20250.15473015824501615No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20100.1535840089246827No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19930.15228503969497148No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19280.14731839264019317No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19240.14701275282143758No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA18700.14288661526823715No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18020.13769073834939216No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17560.1341758804337029No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG17010.12997333292581356No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT16720.12775744423983557No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG16300.12454822614290191No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15170.11591390126305655No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15060.11507339176147868No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15030.114844161897412No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC14880.11369801257707854No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14880.11369801257707854No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT14810.11316314289425626No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14740.11262827321143398No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG14640.11186417366454503No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT14210.10857854561292246No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT13940.10651547683632225No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG13560.10361189855814415No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG13450.10277138905656628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCG450.00884847817.7786259
TAGGACG3000.017.600844
TCTAGCG4700.017.36120428
ATACCGT3800.017.264646
TACCGTC3550.017.1282337
CAACGGA957.696708E-716.84162314
ATCGTTT4850.016.8242629
TAGCGGC4800.016.6661930
ACCGTCG3650.016.6583298
CATCGTT4900.016.65258428
CTAGCGG4850.016.49437329
GTCTTAC1203.1061063E-816.0019861
CGAACGA2300.015.99954216
CGTTTAT5200.015.99954131
CGTCGTA3850.015.79235810
TAAACGC3750.015.78621628
GGACGTG3450.015.7694716
CCGTCGT3900.015.59048759
GTCCTAC2600.015.3865251
GCATCGT5450.015.26561727
Produced by FastQC (version 0.11.5)