FastQCFastQC Report
Thu 2 Feb 2017
SRR4062909_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062909_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085368
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT42380.20322552182636353No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC38650.18533899052829045No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT36580.1754126849553652No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA36120.17320683927249292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33730.16174603235496085No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG33610.1611705943507333No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG32950.15800568532748177No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC31820.152586977454339No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC31730.15215539895116834No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG31360.15038113177146673No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC30020.14395540739092572No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG29400.14098231103575004No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG29090.1394957628581622No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC28990.13901623118797257No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG28480.13657061967000547No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC26330.12626068876092852No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG26270.12597296975881475No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA25590.12271215440152529No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA25280.12122560622393745No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT24580.11786888453261007No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC23540.11288175516263796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23410.11225836399139145No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT23360.11201859815629664No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC22570.10823029796179859No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG22260.10674374978421075No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA21750.10429813826624365No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG21740.1042501850992247No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT21740.1042501850992247No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT21020.10079755707385939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26800.019.8397051
TCTAGCG7800.018.66556228
CTAGCGG8300.017.34878729
TAAACGC6500.017.2297528
GGTATCA10250.017.1870251
GTATAGG1404.0017767E-1117.1591261
AAGACGG8400.017.1385565
CAAGACG8550.017.0249654
CGCAAGA8500.016.5692882
CGCTTCG6700.016.4770332
TAGCGGC8800.016.3630630
AAACGCT6800.016.23472229
ACGCTTC6950.016.1145431
GCGCAAG8650.016.1077581
GCAAGAC10400.015.8436963
AACGCTT7100.015.54874830
GTAAACG7250.015.44736127
TACACCG1255.1591996E-815.3561445
GTCCTAT8450.015.3518331
CGGTCCA9800.015.34713410
Produced by FastQC (version 0.11.5)