##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062909_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2085368
Sequences flagged as poor quality	0
Sequence length	38
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27576859336098	32.0	32.0	32.0	32.0	32.0
2	30.873807404736237	32.0	32.0	32.0	32.0	32.0
3	30.918790352590047	32.0	32.0	32.0	32.0	32.0
4	30.995587829102586	32.0	32.0	32.0	32.0	32.0
5	30.878750417192553	32.0	32.0	32.0	32.0	32.0
6	34.55953529544905	36.0	36.0	36.0	32.0	36.0
7	34.49753185049354	36.0	36.0	36.0	32.0	36.0
8	34.47486103172198	36.0	36.0	36.0	32.0	36.0
9	34.63251138408185	36.0	36.0	36.0	32.0	36.0
10	34.32346616999973	36.0	36.0	36.0	32.0	36.0
11	34.58987526422195	36.0	36.0	36.0	32.0	36.0
12	34.39756052648741	36.0	36.0	36.0	32.0	36.0
13	34.50606655515957	36.0	36.0	36.0	32.0	36.0
14	34.38293481054663	36.0	36.0	36.0	32.0	36.0
15	34.324278017117365	36.0	36.0	36.0	32.0	36.0
16	34.3444734934074	36.0	36.0	36.0	32.0	36.0
17	34.25508543336236	36.0	36.0	36.0	32.0	36.0
18	34.28842487273229	36.0	36.0	36.0	32.0	36.0
19	34.281873031522494	36.0	36.0	36.0	32.0	36.0
20	34.271665720390835	36.0	36.0	36.0	32.0	36.0
21	34.255200041431536	36.0	36.0	36.0	32.0	36.0
22	34.235618845211015	36.0	36.0	36.0	32.0	36.0
23	34.16980072581914	36.0	36.0	36.0	32.0	36.0
24	34.15514767657315	36.0	36.0	36.0	32.0	36.0
25	34.12831787962604	36.0	36.0	36.0	32.0	36.0
26	34.08238258187524	36.0	36.0	36.0	32.0	36.0
27	34.08952089031768	36.0	36.0	36.0	32.0	36.0
28	34.07602159427017	36.0	36.0	36.0	32.0	36.0
29	34.04485155617618	36.0	36.0	36.0	32.0	36.0
30	34.017100578890634	36.0	36.0	36.0	32.0	36.0
31	34.04089446083377	36.0	36.0	36.0	32.0	36.0
32	33.99120874589041	36.0	36.0	36.0	32.0	36.0
33	33.96470311235235	36.0	36.0	36.0	32.0	36.0
34	33.97314622646938	36.0	36.0	36.0	32.0	36.0
35	33.939771781287526	36.0	36.0	36.0	32.0	36.0
36	33.89101012387262	36.0	36.0	36.0	32.0	36.0
37	33.907427370133234	36.0	36.0	36.0	32.0	36.0
38	33.30279308016619	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	7.0
10	20.0
11	21.0
12	11.0
13	11.0
14	170.0
15	427.0
16	551.0
17	632.0
18	732.0
19	1065.0
20	1508.0
21	2334.0
22	3705.0
23	6127.0
24	9234.0
25	14000.0
26	20394.0
27	29037.0
28	41126.0
29	54480.0
30	72947.0
31	98891.0
32	134032.0
33	202432.0
34	451024.0
35	940444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.91601972278635	16.811082784811344	11.231377858435716	26.041519633966594
2	17.609307596607483	18.673536020205034	36.29143190804483	27.425724475142655
3	19.19006139212065	22.39932358841525	28.094244890344495	30.31637012911961
4	12.919745342663353	14.434530660160206	34.78443513488629	37.86128886229015
5	15.538183704922782	35.482308621124545	32.89719886101528	16.08230881293739
6	36.145279123798474	34.42040122472565	15.765345634936809	13.668974016539071
7	31.704667953090294	29.570512254911364	19.97364493940638	18.751174852591966
8	29.352553657350615	31.915513949136887	18.56675243914486	20.165179954367634
9	27.390422007860497	13.775964292085218	18.117652361889817	40.71596133816446
10	16.83464725515019	25.141606245444432	29.97444086392757	28.049305635477808
11	39.165837459388825	20.442904583218443	21.134931005958304	19.25632695143443
12	25.390060038747052	22.98846220244375	27.256248441486196	24.365229317323
13	29.578237556712995	18.016364037485733	25.1510747892147	27.254323616586575
14	24.28925425229322	19.1296724629994	23.541784862521933	33.03928842218544
15	25.958945142356665	26.332062110377198	21.25649141504814	26.452501332218
16	27.210416188081467	24.894263643814632	22.351747293585703	25.5435728745182
17	25.143901920786675	25.351870832887673	23.8135435916941	25.690683654631552
18	26.54148643334644	23.50977815716026	24.872677747619	25.0760576618743
19	26.441014475725527	24.12427495486077	24.3138477327278	25.1208628366859
20	26.654693595685856	23.098307864581212	23.686059365011616	26.560939174721316
21	28.42837576801564	23.130050967656544	23.29583492387462	25.145738340453207
22	27.20282829077261	22.91123657633714	24.0095874913738	25.876347641516446
23	25.12276030483977	22.815408498432497	24.97984568098099	27.081985515746748
24	25.768072527513635	24.106151534466445	24.1278775592226	25.99789837879732
25	25.899475873288726	23.402631760697403	24.16815028546872	26.529742080545155
26	25.613813618218508	24.731013637211703	24.721948702059564	24.93322404251023
27	26.58068556920794	23.497029283224958	23.811725279315475	26.110559868251627
28	25.540343921052962	23.50432514916705	25.01599331685566	25.93933761292433
29	25.35690854470325	23.805684830859306	24.91818248088367	25.919224143553777
30	24.96071310568594	24.252038434739156	25.223456949380452	25.56379151019445
31	26.253302961275626	23.98901810334492	23.41186908044599	26.34580985493346
32	25.91558333189464	23.639429357365238	23.306899773070377	27.13808753766974
33	25.238634183771342	23.383368501822368	24.302608862459238	27.075388451947056
34	26.69869993343322	23.470402719454576	24.24263497039025	25.58826237672196
35	27.066277223828916	23.219905708790762	24.647079418521557	25.066737648858766
36	25.276875588714333	24.191291152450876	23.967795527401712	26.564037731433075
37	26.881427527321488	23.890930820095416	23.863019453551075	25.364622199032024
38	25.548520940475072	23.420424660135698	24.582494902275226	26.448559497114005
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	74.5
2	107.0
3	107.0
4	310.0
5	513.0
6	513.0
7	635.0
8	757.0
9	749.5
10	742.0
11	742.0
12	1018.5
13	1295.0
14	1881.5
15	2468.0
16	2468.0
17	3864.0
18	5260.0
19	5260.0
20	6330.5
21	7401.0
22	8331.5
23	9262.0
24	9262.0
25	10919.5
26	12577.0
27	12577.0
28	16274.0
29	19971.0
30	25470.5
31	30970.0
32	30970.0
33	43446.0
34	55922.0
35	55922.0
36	64211.0
37	72500.0
38	85568.5
39	98637.0
40	98637.0
41	106677.5
42	114718.0
43	136090.5
44	157463.0
45	157463.0
46	159520.5
47	161578.0
48	161578.0
49	177005.0
50	192432.0
51	201050.0
52	209668.0
53	209668.0
54	201886.5
55	194105.0
56	194105.0
57	189703.5
58	185302.0
59	167535.0
60	149768.0
61	149768.0
62	144357.0
63	138946.0
64	116722.5
65	94499.0
66	94499.0
67	79867.0
68	65235.0
69	65235.0
70	52558.0
71	39881.0
72	30926.0
73	21971.0
74	21971.0
75	16826.0
76	11681.0
77	11681.0
78	11241.0
79	10801.0
80	8580.0
81	6359.0
82	6359.0
83	6247.5
84	6136.0
85	6136.0
86	4179.0
87	2222.0
88	1882.0
89	1542.0
90	1542.0
91	1105.5
92	669.0
93	588.0
94	507.0
95	507.0
96	456.5
97	406.0
98	406.0
99	730.5
100	1055.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11978701121336857
2	0.05442684456652255
3	0.011844432253683762
4	0.0026853773530619057
5	9.590633403792521E-5
6	1.438595010568878E-4
7	0.0
8	2.877190021137756E-4
9	1.9181266807585042E-4
10	3.8362533615170084E-4
11	0.0020619861818153918
12	0.002301752016910205
13	0.01476957544184048
14	0.009542680236773557
15	0.02392863034246234
16	0.010981275247342435
17	0.021147346655362508
18	0.00517894203804796
19	0.007336834553901278
20	0.0063777712135220255
21	0.005946192710351363
22	0.007240928219863352
23	0.009926305572925257
24	0.01491343494289737
25	0.018030390799129936
26	0.019708751644793628
27	0.00867952323043223
28	0.0058502863763134376
29	0.011508760084551024
30	0.003212862190270494
31	0.0056584737082375864
32	0.006713443382654764
33	0.008056132059185718
34	0.011700572752626875
35	0.016208170452409357
36	0.015153200777992181
37	0.00939882073571667
38	0.005946192710351363
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2085368.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.23099177088705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.12172318046699	35.654137497127984
2	12.869466148874809	11.899363672486816
3	4.212915535942898	5.8430179042085495
4	1.8447893036790404	3.411457564696247
5	1.0204805065639213	2.358891295065365
6	0.6330895728560486	1.7561015299765428
7	0.4349382052813374	1.4075337212444126
8	0.2867193369252654	1.060425544475691
9	0.22794801174471382	0.9484436389643935
>10	1.1565235344414222	9.363490732725738
>50	0.0773312240748393	2.5145613802038462
>100	0.08475216932244702	8.64998476129917
>500	0.017365198797858132	5.5691964384148935
>1k	0.011958071028465182	9.563394319110435
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	4238	0.20322552182636353	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3865	0.18533899052829045	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3658	0.1754126849553652	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3612	0.17320683927249292	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3373	0.16174603235496085	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3361	0.1611705943507333	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3295	0.15800568532748177	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3182	0.152586977454339	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3173	0.15215539895116834	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3136	0.15038113177146673	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3002	0.14395540739092572	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2940	0.14098231103575004	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2909	0.1394957628581622	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2899	0.13901623118797257	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2848	0.13657061967000547	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2633	0.12626068876092852	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2627	0.12597296975881475	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2559	0.12271215440152529	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2528	0.12122560622393745	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2458	0.11786888453261007	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2354	0.11288175516263796	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2341	0.11225836399139145	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	2336	0.11201859815629664	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	2257	0.10823029796179859	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	2226	0.10674374978421075	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2175	0.10429813826624365	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2174	0.1042501850992247	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2174	0.1042501850992247	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	2102	0.10079755707385939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.79531670189626E-5
2	0.0	0.0	0.0	0.0	4.79531670189626E-5
3	0.0	0.0	0.0	0.0	4.79531670189626E-5
4	0.0	0.0	0.0	0.0	4.79531670189626E-5
5	0.0	0.0	0.0	0.0	4.79531670189626E-5
6	0.0	0.0	0.0	0.0	4.79531670189626E-5
7	0.0	0.0	0.0	4.79531670189626E-5	4.79531670189626E-5
8	0.0	0.0	0.0	9.59063340379252E-5	4.79531670189626E-5
9	0.0	0.0	0.0	9.59063340379252E-5	4.79531670189626E-5
10	0.0	0.0	0.0	9.59063340379252E-5	4.79531670189626E-5
11	0.0	0.0	0.0	9.59063340379252E-5	4.79531670189626E-5
12	0.0	4.79531670189626E-5	0.0	9.59063340379252E-5	9.59063340379252E-5
13	0.0	4.79531670189626E-5	0.0	9.59063340379252E-5	9.59063340379252E-5
14	0.0	4.79531670189626E-5	0.0	9.59063340379252E-5	9.59063340379252E-5
15	0.0	4.79531670189626E-5	0.0	9.59063340379252E-5	1.918126680758504E-4
16	0.0	4.79531670189626E-5	0.0	1.438595010568878E-4	1.918126680758504E-4
17	0.0	4.79531670189626E-5	0.0	1.438595010568878E-4	1.918126680758504E-4
18	0.0	4.79531670189626E-5	0.0	1.438595010568878E-4	2.39765835094813E-4
19	0.0	4.79531670189626E-5	0.0	1.438595010568878E-4	3.356721691327382E-4
20	0.0	4.79531670189626E-5	0.0	1.438595010568878E-4	3.356721691327382E-4
21	0.0	4.79531670189626E-5	0.0	1.918126680758504E-4	3.836253361517008E-4
22	0.0	4.79531670189626E-5	0.0	2.39765835094813E-4	3.836253361517008E-4
23	0.0	4.79531670189626E-5	0.0	3.836253361517008E-4	3.836253361517008E-4
24	0.0	4.79531670189626E-5	0.0	6.233911712465138E-4	3.836253361517008E-4
25	0.0	4.79531670189626E-5	0.0	6.713443382654764E-4	3.836253361517008E-4
26	0.0	4.79531670189626E-5	0.0	9.59063340379252E-4	4.315785031706634E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2680	0.0	19.839705	1
TCTAGCG	780	0.0	18.665562	28
CTAGCGG	830	0.0	17.348787	29
TAAACGC	650	0.0	17.22975	28
GGTATCA	1025	0.0	17.187025	1
GTATAGG	140	4.0017767E-11	17.159126	1
AAGACGG	840	0.0	17.138556	5
CAAGACG	855	0.0	17.024965	4
CGCAAGA	850	0.0	16.569288	2
CGCTTCG	670	0.0	16.47703	32
TAGCGGC	880	0.0	16.36306	30
AAACGCT	680	0.0	16.234722	29
ACGCTTC	695	0.0	16.11454	31
GCGCAAG	865	0.0	16.107758	1
GCAAGAC	1040	0.0	15.843696	3
AACGCTT	710	0.0	15.548748	30
GTAAACG	725	0.0	15.447361	27
TACACCG	125	5.1591996E-8	15.356144	5
GTCCTAT	845	0.0	15.351833	1
CGGTCCA	980	0.0	15.347134	10
>>END_MODULE