FastQCFastQC Report
Thu 2 Feb 2017
SRR4062909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085368
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC43520.20869218286652524No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT42400.2033214281604014No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA37320.1789612193147684No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG35700.17119280625769648No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC35480.1701378365832793No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT35180.16869924157271043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35170.16865128840569146No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC34970.1676922250653122No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC33640.1613144538517902No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG33380.16006767150929718No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG31550.15129224194482702No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG30680.14712031641417725No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC29230.14016710719642766No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG28670.13748172984336576No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA26950.1292337851161042No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG26820.12861039394485768No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG25610.12280806073556322No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC25600.12276010756854426No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT24930.11954724537827377No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG24610.11801274403366697No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA24510.11753321236347733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24460.11729344652838251No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA23860.11441625650724475No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT23850.11436830334022581No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC23550.11292970832965693No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT22730.10899754863410199No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG22660.10866187646496925No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG22130.10612035861296423No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT22010.10554492060873667No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC21800.10453790410133847No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC21670.10391451293009196No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC21660.10386655976307299No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG21520.1031952154248075No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC21430.10276363692163686No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT20920.10031802540366976No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG20900.10022211906963183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27900.018.7543741
TCTAGCG7350.018.72052828
CTAGCGG7650.018.19553429
GGTATCA11950.018.0769391
ACTAGAC1151.8428182E-816.6959533
CAAGACG9550.016.5870844
TAGCGGC8400.016.57093230
AAGACGG10000.016.1614535
GCGTACG600.00244133516.001449
TACCGTC8350.015.9056227
ACCGTCG8000.015.8014218
ACACCGT1950.015.59114556
ATACCGT8550.015.3464086
CGTCGTA8400.015.23873410
CGCGGTA4000.015.19954531
CTTACAC2750.015.1275443
CCGTCGT8500.015.0601789
CGCAAGA10000.015.0395492
GACGGAC10550.014.8638967
ATTTGCC10250.014.67273126
Produced by FastQC (version 0.11.5)