FastQCFastQC Report
Thu 2 Feb 2017
SRR4062908_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062908_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704691
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27000.3831466557682729No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19760.28040658955485454No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15810.22435365287764422No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11870.16844262236923702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10310.14630526003595903No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8820.12516124088430247No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.12303264835225652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT200.00375612531.9937574
GTAATAG555.8379497E-520.3596633
CTAGCGG951.720764E-920.21088629
TAAGGCG400.00448685119.9960985
TCTAGCG1003.25781E-919.19897728
GCATTCG1600.018.9989922
ATTCGTA1700.018.82252924
CGTGTTG601.1366146E-418.66832214
GACGGCC1650.018.42587711
ATACCGT1159.422365E-1018.0834266
CGTATTG1700.017.88140127
CTCTTAC450.00881356117.7894551
CGGTGTT450.0088424517.77935414
CCTTAAG450.00884968317.7768332
TTCGTAT1800.017.77683325
CGTCGTA1101.0599251E-817.45609310
AACGAAT550.001363773117.45485531
ACCGTCG1101.0617441E-817.4536178
GACTGTA550.001364421917.4536177
AACAGCG550.001364421917.4536177
Produced by FastQC (version 0.11.5)