FastQCFastQC Report
Thu 2 Feb 2017
SRR4062908_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062908_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704691
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30250.4292661606292687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21920.3110583220163164No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15160.2151297519054451No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14870.21101447301015622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13260.18816757983286292No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.14616335386715595No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8150.11565352757449719No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC7070.10032766134376629No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATAG350.00206420822.860083
CAATAGG453.531822E-421.3360791
ATAATAC707.765939E-720.5740743
TAGCGGC1203.6379788E-1219.99831630
TCTAGCG1203.6379788E-1219.99831628
CTAGCGG1203.6379788E-1219.99831629
GTCCTAA1800.018.6690671
TAATACT802.7896385E-618.0023164
TACACCG450.00883318917.7825895
ACTATAC550.001362760217.456793
ATCGTTT1601.8189894E-1216.99856829
CCGACTG957.688777E-716.84068725
TAAACGC1151.8391802E-816.69424628
CATCGTT1651.8189894E-1216.4834628
ACCGTGC600.002436242516.0043329
CACGCAT1001.3089048E-616.004339
TCTAGGC600.002438552216.0020583
GTACTAT908.5609045E-616.0020581
GTGTTAG703.6902472E-416.0020561
GGTAAAA908.578225E-615.99865215
Produced by FastQC (version 0.11.5)