FastQCFastQC Report
Thu 2 Feb 2017
SRR4062907_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062907_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1689161
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT53340.31577806970442723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35230.20856508053406392No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19810.11727715712119804No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19420.11496831859130065No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19350.11455391167567804No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19020.11260027907345718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18830.11147546030248154No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17710.10484494965251981No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17640.1044305427368972No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17620.10431214076100502No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17390.10295051803824504No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17100.1012336893878085No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17070.10105608642397024No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16920.10016807160477895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTTA400.004485128419.9993913
ACCGTCG3650.016.6560388
TACCGTC3650.016.6555467
CCGTCGT3900.016.4097569
GTATAGG1304.7130015E-916.0237051
TCTATAC1407.421477E-1016.0009333
TACGACA600.002444489415.9980914
ATACCGT4250.015.4320826
CGTCGTA4050.015.40693810
TCGTTTA3950.014.98599730
CATCGTT3850.014.95969828
GTAATAC1401.27874955E-814.8580093
TCGCGTA1951.8189894E-1214.768789
GCGTAAC1951.8189894E-1214.7687811
ATCGTTT3900.014.76790429
TTAAGCG650.004165139514.76790419
GTATTAG4750.014.50567151
CGCGTAA2003.6379788E-1214.39956110
GTCCTAT6550.013.9442931
TCTAGAT4600.013.9216862
Produced by FastQC (version 0.11.5)