##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062907_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1689161
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178209773964706	32.0	32.0	32.0	32.0	32.0
2	30.750715295936857	32.0	32.0	32.0	32.0	32.0
3	30.791703099941333	32.0	32.0	32.0	32.0	32.0
4	30.877030667887787	32.0	32.0	32.0	32.0	32.0
5	30.77014624420052	32.0	32.0	32.0	32.0	32.0
6	34.43114658697424	36.0	36.0	36.0	32.0	36.0
7	34.34864882625161	36.0	36.0	36.0	32.0	36.0
8	34.31882396053425	36.0	36.0	36.0	32.0	36.0
9	34.474774163031235	36.0	36.0	36.0	32.0	36.0
10	34.17168819313257	36.0	36.0	36.0	32.0	36.0
11	34.48973129263581	36.0	36.0	36.0	32.0	36.0
12	34.27428350524313	36.0	36.0	36.0	32.0	36.0
13	34.37668700615276	36.0	36.0	36.0	32.0	36.0
14	34.263347898749736	36.0	36.0	36.0	32.0	36.0
15	34.20523028888306	36.0	36.0	36.0	32.0	36.0
16	34.215669791097476	36.0	36.0	36.0	32.0	36.0
17	34.13505106973225	36.0	36.0	36.0	32.0	36.0
18	34.16690652933616	36.0	36.0	36.0	32.0	36.0
19	34.15999540600333	36.0	36.0	36.0	32.0	36.0
20	34.1387304111331	36.0	36.0	36.0	32.0	36.0
21	34.13015870008839	36.0	36.0	36.0	32.0	36.0
22	34.09929840909185	36.0	36.0	36.0	32.0	36.0
23	34.04795161621657	36.0	36.0	36.0	32.0	36.0
24	34.02643146508829	36.0	36.0	36.0	32.0	36.0
25	34.00715917547232	36.0	36.0	36.0	32.0	36.0
26	33.95942660291115	36.0	36.0	36.0	32.0	36.0
27	33.97024795149782	36.0	36.0	36.0	32.0	36.0
28	33.945791431367404	36.0	36.0	36.0	32.0	36.0
29	33.91460139086801	36.0	36.0	36.0	32.0	36.0
30	33.88878502404448	36.0	36.0	36.0	32.0	36.0
31	33.90061634148551	36.0	36.0	36.0	32.0	36.0
32	33.852225453938374	36.0	36.0	36.0	32.0	36.0
33	33.81836544888261	36.0	36.0	36.0	32.0	36.0
34	33.81814640522721	36.0	36.0	36.0	32.0	36.0
35	33.781511057856534	36.0	36.0	36.0	32.0	36.0
36	33.72556079615857	36.0	36.0	36.0	27.0	36.0
37	33.729277434181824	36.0	36.0	36.0	27.0	36.0
38	33.12194456301087	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	3.0
8	2.0
9	9.0
10	5.0
11	12.0
12	11.0
13	10.0
14	174.0
15	423.0
16	542.0
17	650.0
18	892.0
19	1165.0
20	1587.0
21	2405.0
22	3602.0
23	5630.0
24	8249.0
25	12700.0
26	18212.0
27	25922.0
28	35736.0
29	48239.0
30	64250.0
31	85344.0
32	115217.0
33	171381.0
34	368797.0
35	717991.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.09496512640382	17.432293299776596	11.625855836044604	25.846885737774976
2	16.661829184353095	19.880512212372185	37.36913623100499	26.088522372269736
3	18.473640676705664	24.34066906776826	28.787818682291395	28.397871573234685
4	12.670497097575343	15.51454764505463	36.04589412736331	35.76906113000672
5	14.728962114193193	36.396600667197504	33.401967255817254	15.472469962792045
6	33.77493402038175	35.6623832702447	16.968809312095136	13.593873397278408
7	29.88213076321959	30.751675388950723	21.21835705320988	18.147836794619813
8	28.2560215018752	32.33214240256223	19.778528317413144	19.63330777814943
9	27.82160310924693	14.101192608138389	18.759971701827244	39.31723258078744
10	16.161541412495737	26.381936024703517	31.399187284507256	26.057335278293486
11	37.19713012533649	21.30341423677117	22.34951303420157	19.14994260369077
12	24.917856927191934	23.75849995796643	28.552221681508662	22.77142143333298
13	29.46355194194647	19.36913679952443	25.32932210093527	25.837989157593828
14	23.996329188869154	19.618886915334517	25.130550621669627	31.2542332741267
15	25.284156684543596	26.64662840210828	22.64756162624965	25.421653287098472
16	25.846991494264575	25.660725006483208	23.58686732450038	24.90541617475183
17	24.104114559648323	25.56358477338184	25.283805612399572	25.048495054570257
18	25.112043640479932	24.453043501942485	26.237867612668243	24.19704524490934
19	25.749331868199533	24.85284522905938	25.608008819221357	23.78981408351973
20	25.916231761359093	23.914473392083785	25.13437907954993	25.034915767007192
21	27.16937812413599	24.059791589084387	24.488134573702524	24.2826957130771
22	25.973244004589585	24.022789249764067	24.99203690612785	25.011929839518498
23	24.482136545319232	23.813637243658555	25.59870977444143	26.105516436580782
24	24.83510367449406	24.96483001172333	25.177981455824362	25.022084857958244
25	24.99914144793326	24.235977476330607	25.31354913582294	25.451331939913196
26	24.840897840663363	25.07200157266593	25.655237995987807	24.4318625906829
27	25.432292610262298	24.65568750599461	24.753495818280207	25.15852406546289
28	24.5577598573417	24.451192401029914	25.77191840958728	25.219129332041106
29	24.579884298332917	24.916474195662637	25.60380438354315	24.899837122461292
30	24.652377132403654	24.836378813757502	25.89598695174692	24.615257102091924
31	25.1973864938854	24.68106747373706	24.681955535347218	25.439590497030323
32	24.755528110788973	24.82894220357136	24.682824476968992	25.732705208670676
33	24.52016724431714	24.407853993958646	25.361066081872035	25.71091267985218
34	25.26621490803485	24.526526757669682	25.43596484216572	24.771293492129747
35	25.975213261751673	24.380316741143723	25.282863803202936	24.361606193901665
36	24.77738613816832	25.181255850663536	24.85447723414674	25.186880777021408
37	25.61132073460915	24.994212575791575	24.912093318736986	24.482373370862287
38	24.748707868827225	24.803294120433137	25.321271468914848	25.12672654182479
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	541.0
1	438.5
2	336.0
3	336.0
4	808.5
5	1281.0
6	1281.0
7	1529.5
8	1778.0
9	1683.0
10	1588.0
11	1588.0
12	2096.5
13	2605.0
14	3407.0
15	4209.0
16	4209.0
17	6356.0
18	8503.0
19	8503.0
20	10203.5
21	11904.0
22	12495.5
23	13087.0
24	13087.0
25	14994.5
26	16902.0
27	16902.0
28	21445.5
29	25989.0
30	31493.5
31	36998.0
32	36998.0
33	46701.5
34	56405.0
35	56405.0
36	62992.0
37	69579.0
38	79915.5
39	90252.0
40	90252.0
41	96675.0
42	103098.0
43	117406.5
44	131715.0
45	131715.0
46	136420.0
47	141125.0
48	141125.0
49	149084.0
50	157043.0
51	158139.0
52	159235.0
53	159235.0
54	151457.0
55	143679.0
56	143679.0
57	139663.5
58	135648.0
59	121802.0
60	107956.0
61	107956.0
62	101957.5
63	95959.0
64	79108.5
65	62258.0
66	62258.0
67	51972.5
68	41687.0
69	41687.0
70	33691.0
71	25695.0
72	20366.0
73	15037.0
74	15037.0
75	11371.0
76	7705.0
77	7705.0
78	7423.0
79	7141.0
80	5517.0
81	3893.0
82	3893.0
83	3994.5
84	4096.0
85	4096.0
86	2739.5
87	1383.0
88	1188.0
89	993.0
90	993.0
91	743.5
92	494.0
93	408.5
94	323.0
95	323.0
96	298.0
97	273.0
98	273.0
99	520.5
100	768.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12242764307250759
2	0.05653694348851294
3	0.012669011420462584
4	0.0033152553249808634
5	3.552059276765211E-4
6	1.7760296383826054E-4
7	5.9200987946086846E-5
8	3.552059276765211E-4
9	3.552059276765211E-4
10	5.328088915147817E-4
11	0.0016576276624904317
12	0.0020720345781130396
13	0.014030634143222582
14	0.009531359059319983
15	0.023206787274866043
16	0.010833780794133894
17	0.019477125034262573
18	0.005150485951309556
19	0.007281721517368681
20	0.005683294842824337
21	0.005564892866932163
22	0.007044917565584335
23	0.009116952143697375
24	0.013083418336085192
25	0.016043467733389535
26	0.018293105275340837
27	0.00846574127629042
28	0.005328088915147816
29	0.00959056004726607
30	0.002723245445519995
31	0.005268887927201729
32	0.006630510649961727
33	0.007992133372721723
34	0.012195403516893891
35	0.016221070697227797
36	0.01480024698652171
37	0.009116952143697375
38	0.0053872899030939034
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1689161.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.93233223310124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.74607666154998	43.806379774586254
2	12.289995558164264	13.502362382888359
3	3.6121266307719426	5.95267620448791
4	1.5050532488904966	3.3070434038624477
5	0.7588766281020896	2.08434315294198
6	0.463243052520519	1.5268212759439874
7	0.30684616076027277	1.1799042673134428
8	0.20925791855587686	0.9196020403614936
9	0.15616874628861974	0.7720842109998022
>10	0.7927576770432864	7.692748937157912
>50	0.07029901174467586	2.7057888494343754
>100	0.07468649356160441	8.940752275255171
>500	0.010499144951822009	3.967572322418685
>1k	0.0040048284867774675	3.324771975078422
>5k	1.0823860775074235E-4	0.3171489272697218
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5334	0.31577806970442723	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3523	0.20856508053406392	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1981	0.11727715712119804	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1942	0.11496831859130065	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1935	0.11455391167567804	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1902	0.11260027907345718	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1883	0.11147546030248154	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1771	0.10484494965251981	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1764	0.1044305427368972	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1762	0.10431214076100502	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1739	0.10295051803824504	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1710	0.1012336893878085	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1707	0.10105608642397024	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1692	0.10016807160477895	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	5.9200987946086846E-5
6	0.0	0.0	0.0	0.0	5.9200987946086846E-5
7	0.0	0.0	0.0	0.0	5.9200987946086846E-5
8	0.0	0.0	0.0	0.0	5.9200987946086846E-5
9	0.0	0.0	0.0	0.0	5.9200987946086846E-5
10	0.0	0.0	0.0	0.0	5.9200987946086846E-5
11	0.0	0.0	0.0	0.0	5.9200987946086846E-5
12	0.0	0.0	0.0	0.0	1.7760296383826054E-4
13	0.0	0.0	0.0	0.0	2.960049397304342E-4
14	0.0	0.0	0.0	5.9200987946086846E-5	4.7360790356869477E-4
15	0.0	0.0	0.0	5.9200987946086846E-5	6.512108674069553E-4
16	0.0	0.0	0.0	1.1840197589217369E-4	7.104118553530422E-4
17	0.0	0.0	0.0	1.1840197589217369E-4	7.104118553530422E-4
18	0.0	0.0	0.0	1.7760296383826054E-4	8.288138312452159E-4
19	0.0	0.0	0.0	4.1440691562260793E-4	8.288138312452159E-4
20	0.0	0.0	0.0	4.7360790356869477E-4	8.288138312452159E-4
21	0.0	0.0	0.0	5.920098794608684E-4	8.288138312452159E-4
22	0.0	0.0	0.0	0.001065617783029563	8.288138312452159E-4
23	0.0	0.0	0.0	0.0014208237107060844	8.288138312452159E-4
24	0.0	0.0	0.0	0.0026048434696278214	8.288138312452159E-4
25	0.0	0.0	0.0	0.003019250385250429	8.288138312452159E-4
26	0.0	0.0	0.0	0.0040848681682799926	8.288138312452159E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCTTA	40	0.0044851284	19.99939	13
ACCGTCG	365	0.0	16.656038	8
TACCGTC	365	0.0	16.655546	7
CCGTCGT	390	0.0	16.409756	9
GTATAGG	130	4.7130015E-9	16.023705	1
TCTATAC	140	7.421477E-10	16.000933	3
TACGACA	60	0.0024444894	15.998091	4
ATACCGT	425	0.0	15.432082	6
CGTCGTA	405	0.0	15.406938	10
TCGTTTA	395	0.0	14.985997	30
CATCGTT	385	0.0	14.959698	28
GTAATAC	140	1.27874955E-8	14.858009	3
TCGCGTA	195	1.8189894E-12	14.76878	9
GCGTAAC	195	1.8189894E-12	14.76878	11
ATCGTTT	390	0.0	14.767904	29
TTAAGCG	65	0.0041651395	14.767904	19
GTATTAG	475	0.0	14.5056715	1
CGCGTAA	200	3.6379788E-12	14.399561	10
GTCCTAT	655	0.0	13.944293	1
TCTAGAT	460	0.0	13.921686	2
>>END_MODULE