FastQCFastQC Report
Thu 2 Feb 2017
SRR4062907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1689161
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT54990.32554623271553157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT38300.22673978383351262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT23320.13805670389027452No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22390.13255101201128844No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20650.12225004010866934No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19870.11763236304887456No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA19800.11721795613325195No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19600.11603393637433022No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18960.11224507314578065No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18510.10958102868820677No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18170.10756819509803979No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17530.10377933186949025No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17420.10312812100208328No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG17040.10087848346013199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAATA450.00885043917.7783033
TCTAGCG4000.017.59947828
AACGCTT4750.016.84160630
CTAGCGG4200.016.76140829
TAAACGC4800.016.3328528
CGTGCGG805.621941E-516.00047510
CGCGTAA1703.6379788E-1216.00047310
ACGCTTC4900.015.99952731
GCGATGC4600.015.99952632
TAGCGGC4550.015.82370830
TGGCGAT4800.015.66620330
GTCCTAT6650.015.3998641
CGCTTCG5000.015.35954732
TCGCGTA1807.2759576E-1215.1120049
CGTTTAT3650.014.90366831
CTATTCC6150.014.8297074
CCTATTC6150.014.8297073
GTAAACG5300.014.79201527
GCGTAAC1851.0913936E-1114.70313711
AAACGCT5450.014.67846529
Produced by FastQC (version 0.11.5)