FastQCFastQC Report
Thu 2 Feb 2017
SRR4062906_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062906_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71383
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29854.18166790412283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22203.1099841699003967No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13691.9178235714385778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12501.7511172127817547No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10801.512965271843436No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9571.3406553381057114No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9061.269209755824216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6880.9638149139150778No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5810.8139192805009595No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA5150.7214602916660829No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG5080.7116540352745051No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4650.6514156031548128No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.5673619769412885No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.5589566143199362No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3940.5519521454688091No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3760.5267360576047518No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA3640.509925332362047No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2990.4188672372973957No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2820.3950520432035639No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA2740.38384489304176067No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG2720.3810431055013098No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC2130.298390373058011No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA2110.2955885855175602No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC1980.27737696650462995No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1950.2731742851939537No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1870.2619671350321505No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA1870.2619671350321505No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA1790.2507599848703473No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1660.232548365857417No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA1610.22554389700629002No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1570.2199403219253884No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT1550.21713853438493758No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1540.21573764061471218No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG1500.21013406553381056No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA1390.19472423406133113No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1370.19192244652088034No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA1330.18631887143997872No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1270.17791350881862628No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC1210.16950814619727386No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA1210.16950814619727386No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1170.16390457111637224No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1140.15970188980569602No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1120.15690010226524523No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1110.15549920849501983No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1090.152697420954569No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1090.152697420954569No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT1080.1512965271843436No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA1070.1498956334141182No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1050.14709384587366742No Hit
GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAA1040.145692952103442No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1020.1428911645629912No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA990.13868848325231498No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA980.13728758948208958No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC960.13448580194163878No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC950.13308490817141336No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA930.13028312063096256No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG920.12888222686073716No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT870.12187775800961012No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT850.11907597046915933No Hit
GAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAA830.1162741829287085No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA830.1162741829287085No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT820.1148732891584831No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA800.11207150161803231No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG790.1106706078478069No Hit
GAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAA770.10786882030735609No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT720.10086435145622909No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT720.10086435145622909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGCC507.111096E-419.19789911
AAAAAGT701.7090691E-518.3351081
ACTGCCC450.0087769917.7758312
AAAAGTA951.3875171E-515.1668642
GGGAGTG1951.8189894E-1214.7676137
TGGGTGT650.004113944214.7676136
AAGTACT1002.268264E-514.3984234
AAAGTAC1002.268264E-514.3984233
TGGGCAG800.001002438513.99846656
ATGGGTG2900.013.7915945
TGGGTGG2350.013.6155316
AGTACTC952.3795442E-413.4722095
TGGGAGT2400.013.3318756
TTTGGTT850.001584013213.17502832
ATGGGAG3050.013.1133185
CATGGGT3300.013.0894754
GGAGTGG2252.0008883E-1112.7985998
ATGGAAG4200.012.5700525
CACTGCT5200.012.30634423
TTTTGGT1055.5403967E-412.18914131
Produced by FastQC (version 0.11.5)