##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062906_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71383
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.58627404283933	32.0	32.0	32.0	14.0	32.0
2	29.642744070717118	32.0	32.0	32.0	14.0	32.0
3	29.96034069736492	32.0	32.0	32.0	21.0	32.0
4	29.717916030427414	32.0	32.0	32.0	21.0	32.0
5	30.054368687222446	32.0	32.0	32.0	21.0	32.0
6	33.292954905229536	36.0	36.0	36.0	21.0	36.0
7	32.70801171147192	36.0	36.0	36.0	14.0	36.0
8	32.55927881988709	36.0	36.0	36.0	14.0	36.0
9	32.33538797752966	36.0	32.0	36.0	14.0	36.0
10	32.60304274126893	36.0	36.0	36.0	14.0	36.0
11	33.11226762674586	36.0	36.0	36.0	21.0	36.0
12	32.92624294299763	36.0	36.0	36.0	21.0	36.0
13	32.99795469509547	36.0	36.0	36.0	21.0	36.0
14	32.912500175111724	36.0	36.0	36.0	21.0	36.0
15	33.03094574338428	36.0	36.0	36.0	21.0	36.0
16	32.929030721600384	36.0	36.0	36.0	21.0	36.0
17	32.83313954302845	36.0	36.0	36.0	21.0	36.0
18	32.855707941666786	36.0	36.0	36.0	14.0	36.0
19	32.80933835787232	36.0	36.0	36.0	14.0	36.0
20	32.542440076768976	36.0	36.0	36.0	14.0	36.0
21	32.39484190913803	36.0	32.0	36.0	14.0	36.0
22	32.441029376742364	36.0	32.0	36.0	14.0	36.0
23	32.49527198352549	36.0	32.0	36.0	14.0	36.0
24	32.55319894092431	36.0	32.0	36.0	14.0	36.0
25	32.54150147794293	36.0	36.0	36.0	14.0	36.0
26	32.517055881652496	36.0	36.0	36.0	14.0	36.0
27	32.544037095667036	36.0	36.0	36.0	14.0	36.0
28	32.493506857375	36.0	36.0	36.0	14.0	36.0
29	32.42070240813639	36.0	36.0	36.0	14.0	36.0
30	32.24180827367861	36.0	32.0	36.0	14.0	36.0
31	32.246893518064525	36.0	32.0	36.0	14.0	36.0
32	32.11254780549991	36.0	32.0	36.0	14.0	36.0
33	31.86209601725901	36.0	32.0	36.0	14.0	36.0
34	31.683678186683103	36.0	32.0	36.0	14.0	36.0
35	31.396999285544176	36.0	32.0	36.0	14.0	36.0
36	31.303377554880015	36.0	32.0	36.0	14.0	36.0
37	31.202219015732037	36.0	32.0	36.0	14.0	36.0
38	30.287141196083102	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	1.0
14	119.0
15	207.0
16	256.0
17	288.0
18	373.0
19	421.0
20	454.0
21	587.0
22	798.0
23	945.0
24	1057.0
25	1217.0
26	1487.0
27	1995.0
28	2520.0
29	3290.0
30	3983.0
31	5075.0
32	6098.0
33	7832.0
34	12632.0
35	19745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	54.06061116019465	17.19606770723772	14.343612829035019	14.399708303532613
2	11.810217955007358	25.886887658560514	41.16756605228117	21.13532833415096
3	18.97102505184106	39.55332623437763	26.513198453174912	14.9624502606064
4	9.591056192997941	19.17510752462209	32.57540733268889	38.658428949691086
5	8.859376313356309	45.73141688380824	28.03507887142417	17.37412793141128
6	24.125129584489084	35.445910733798435	22.834888347202376	17.5940713345101
7	23.91325665774764	37.604191474160515	21.687236456859477	16.795315411232366
8	33.516383452642785	25.660171189218723	21.212333468753066	19.611111889385427
9	32.212151352562934	14.525867503467213	20.769651037361836	32.492330106608016
10	18.374122690276394	30.80005043217573	28.187383550705352	22.638443326842523
11	30.221904681852568	27.930010366759127	20.524782157966996	21.323302793421313
12	26.133736813348463	30.768153098250185	28.49997898541588	14.598131102985473
13	32.30113915005114	25.002452045005537	27.80058568846418	14.895823116479143
14	27.348128222371663	21.369928267204664	31.93790629903609	19.344037211387583
15	24.209641255605383	25.00140134529148	36.91844170403588	13.870515695067265
16	17.791694687289844	29.962452364940596	32.36522080251065	19.88063214525891
17	16.484040019056696	27.5467309363002	33.16285065717569	22.806378387467422
18	18.232508616100198	24.663061447504834	39.84280870856566	17.261621227829302
19	17.8407307778525	29.88399462004035	38.8001569154898	13.475117686617349
20	21.758805234105747	20.65202163131497	42.174059233937626	15.415113900641655
21	24.105078809106832	23.77443082311734	34.20105078809107	17.919439579684763
22	20.91179107823358	25.77897332436673	35.1630800268998	18.14615557049989
23	17.251380054361533	28.108274721887522	35.96127441365203	18.679070810098917
24	16.15221868826274	31.870087290356025	36.07207409171792	15.90561992966331
25	20.132544975620693	27.00919127949336	35.04315417810906	17.815109566776886
26	16.16967013256355	27.3366777836944	38.133740646282334	18.35991143745971
27	18.359369527145358	30.499474605954468	36.969527145359024	14.171628721541154
28	16.77010801798899	27.995012398952046	38.216792524202475	17.018087058856494
29	14.179031286340773	31.391422526725794	40.22669636977568	14.202849817157748
30	17.635438994662294	29.630563611146137	38.515529777665705	14.218467616525867
31	14.366374781085813	31.485814360770576	36.64588441330998	17.501926444833625
32	12.952351597853651	33.27262283368592	37.24308951062667	16.531936057833757
33	14.158622525463386	32.71081721002564	35.984981156394916	17.14557910811606
34	13.22488896361369	33.87275300183543	37.22556148683676	15.676796547714122
35	16.068826364757314	33.17172962672346	35.94187871314875	14.817565295370475
36	15.975452558426273	35.18886958471109	34.55837022922154	14.277307627641092
37	16.108106593437384	36.497884383669124	34.32762630649817	13.066382716395324
38	14.68861646234676	37.47250437828371	32.59264448336252	15.246234676007006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	830.0
1	594.0
2	358.0
3	358.0
4	765.0
5	1172.0
6	1172.0
7	1271.0
8	1370.0
9	1249.5
10	1129.0
11	1129.0
12	1427.0
13	1725.0
14	1918.5
15	2112.0
16	2112.0
17	2973.5
18	3835.0
19	3835.0
20	3963.5
21	4092.0
22	3220.5
23	2349.0
24	2349.0
25	1863.5
26	1378.0
27	1378.0
28	1873.5
29	2369.0
30	2820.0
31	3271.0
32	3271.0
33	2801.5
34	2332.0
35	2332.0
36	2264.0
37	2196.0
38	2578.0
39	2960.0
40	2960.0
41	3480.5
42	4001.0
43	4670.5
44	5340.0
45	5340.0
46	7844.5
47	10349.0
48	10349.0
49	8447.5
50	6546.0
51	4639.0
52	2732.0
53	2732.0
54	2223.0
55	1714.0
56	1714.0
57	1614.5
58	1515.0
59	1441.0
60	1367.0
61	1367.0
62	1266.5
63	1166.0
64	1061.0
65	956.0
66	956.0
67	864.0
68	772.0
69	772.0
70	667.0
71	562.0
72	456.0
73	350.0
74	350.0
75	287.0
76	224.0
77	224.0
78	170.5
79	117.0
80	94.0
81	71.0
82	71.0
83	57.5
84	44.0
85	44.0
86	29.5
87	15.0
88	10.5
89	6.0
90	6.0
91	5.5
92	5.0
93	5.0
94	5.0
95	5.0
96	3.0
97	1.0
98	1.0
99	24.0
100	47.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10646792653713069
2	0.053233963268565346
3	0.015409831472479443
4	0.005603575080901615
5	0.0014008937702254037
6	0.0014008937702254037
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0014008937702254037
12	0.005603575080901615
13	0.019612512783155654
14	0.009806256391577827
15	0.03222055671518429
16	0.009806256391577827
17	0.023815194093831864
18	0.007004468851127019
19	0.009806256391577827
20	0.007004468851127019
21	0.01120715016180323
22	0.009806256391577827
23	0.012608043932028636
24	0.016810725242704848
25	0.015409831472479443
26	0.029418769174733482
27	0.01120715016180323
28	0.008405362621352424
29	0.014008937702254039
30	0.005603575080901615
31	0.01120715016180323
32	0.008405362621352424
33	0.008405362621352424
34	0.014008937702254039
35	0.021013406553381055
36	0.015409831472479443
37	0.012608043932028636
38	0.01120715016180323
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	71383.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.89584354818374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.2969296055916	37.396859196167156
2	9.541937094358461	8.567866298698569
3	2.7926859710434346	3.7613997730552096
4	1.1451572641038443	2.056512054690893
5	0.6490264603095357	1.45692952103442
6	0.48676984523215183	1.311236568930978
7	0.2465052421367948	0.7746942549346483
8	0.2683474787818273	0.9638149139150778
9	0.1747378931602596	0.7060504601936035
>10	1.1077134298552171	9.632545564069876
>50	0.13105341987019473	4.124231259543589
>100	0.12481278082875688	10.953588389392433
>500	0.01872191712431353	5.820713615286553
>1k	0.01560159760359461	12.473558130086996
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2985	4.18166790412283	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2220	3.1099841699003967	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1369	1.9178235714385778	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1250	1.7511172127817547	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1080	1.512965271843436	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	957	1.3406553381057114	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	906	1.269209755824216	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	688	0.9638149139150778	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	581	0.8139192805009595	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	515	0.7214602916660829	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	508	0.7116540352745051	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	465	0.6514156031548128	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	405	0.5673619769412885	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	399	0.5589566143199362	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	394	0.5519521454688091	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	376	0.5267360576047518	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	364	0.509925332362047	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	299	0.4188672372973957	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	282	0.3950520432035639	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	274	0.38384489304176067	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	272	0.3810431055013098	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	213	0.298390373058011	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	211	0.2955885855175602	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	198	0.27737696650462995	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	195	0.2731742851939537	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	187	0.2619671350321505	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	187	0.2619671350321505	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	179	0.2507599848703473	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	166	0.232548365857417	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	161	0.22554389700629002	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	157	0.2199403219253884	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	155	0.21713853438493758	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	154	0.21573764061471218	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	150	0.21013406553381056	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	139	0.19472423406133113	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	137	0.19192244652088034	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	133	0.18631887143997872	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	127	0.17791350881862628	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	121	0.16950814619727386	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	121	0.16950814619727386	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	117	0.16390457111637224	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	114	0.15970188980569602	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	112	0.15690010226524523	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA	111	0.15549920849501983	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	109	0.152697420954569	No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	109	0.152697420954569	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	108	0.1512965271843436	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	107	0.1498956334141182	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	105	0.14709384587366742	No Hit
GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAA	104	0.145692952103442	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	102	0.1428911645629912	No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA	99	0.13868848325231498	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	98	0.13728758948208958	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	96	0.13448580194163878	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	95	0.13308490817141336	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	93	0.13028312063096256	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	92	0.12888222686073716	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	87	0.12187775800961012	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	85	0.11907597046915933	No Hit
GAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAA	83	0.1162741829287085	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	83	0.1162741829287085	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	82	0.1148732891584831	No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	80	0.11207150161803231	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	79	0.1106706078478069	No Hit
GAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAA	77	0.10786882030735609	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	72	0.10086435145622909	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	72	0.10086435145622909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.001400893770225404	0.0
17	0.0	0.0	0.0	0.001400893770225404	0.0
18	0.0	0.0	0.0	0.001400893770225404	0.0
19	0.0	0.0	0.0	0.002801787540450808	0.0
20	0.0	0.0	0.0	0.002801787540450808	0.0
21	0.0	0.0	0.0	0.002801787540450808	0.0
22	0.0	0.0	0.0	0.014008937702254039	0.0
23	0.0	0.0	0.0	0.03362145048540969	0.0
24	0.0	0.0	0.0	0.056035750809016155	0.0
25	0.0	0.0	0.0	0.05883753834946696	0.0
26	0.0	0.0	0.0	0.07985094490284801	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTGCC	50	7.111096E-4	19.197899	11
AAAAAGT	70	1.7090691E-5	18.335108	1
ACTGCCC	45	0.00877699	17.77583	12
AAAAGTA	95	1.3875171E-5	15.166864	2
GGGAGTG	195	1.8189894E-12	14.767613	7
TGGGTGT	65	0.0041139442	14.767613	6
AAGTACT	100	2.268264E-5	14.398423	4
AAAGTAC	100	2.268264E-5	14.398423	3
TGGGCAG	80	0.0010024385	13.9984665	6
ATGGGTG	290	0.0	13.791594	5
TGGGTGG	235	0.0	13.615531	6
AGTACTC	95	2.3795442E-4	13.472209	5
TGGGAGT	240	0.0	13.331875	6
TTTGGTT	85	0.0015840132	13.175028	32
ATGGGAG	305	0.0	13.113318	5
CATGGGT	330	0.0	13.089475	4
GGAGTGG	225	2.0008883E-11	12.798599	8
ATGGAAG	420	0.0	12.570052	5
CACTGCT	520	0.0	12.306344	23
TTTTGGT	105	5.5403967E-4	12.189141	31
>>END_MODULE