FastQCFastQC Report
Thu 2 Feb 2017
SRR4062906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71383
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35334.949357690206352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26433.7025622347057427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15632.189596962862306No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13091.8337699452250535No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12991.8197610075227997No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11931.6712662678789065No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9921.3896866200636004No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.8867657565526808No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.8349326870543408No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG5510.7718924673941975No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5140.7200593978958576No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.6878388411806733No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA4890.6850370536402225No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4160.582771808413768No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA4150.5813709146435425No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA3980.5575557205497107No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA3790.5309387389154281No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3700.5183306949833993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3690.516929801213174No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3130.43847975008055134No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG3040.42587170614852277No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC2810.39365114943333845No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA2700.378241317960859No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC2600.364232380258605No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2350.3292100360029699No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA2130.298390373058011No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA2070.2899850104366586No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA2060.2885841166664332No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG1940.27177339142372836No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1850.25916534749169967No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA1840.25776445372147433No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC1830.2563635599512489No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.2521608786405727No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT1670.23394925962764243No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA1670.23394925962764243No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA1590.2227421094658392No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1580.2213412156956138No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1510.21153495930403599No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1460.20453049045290897No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1450.20312959668268354No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA1420.19892691537200732No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1400.19612512783155653No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA1340.18771976521020411No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1330.18631887143997872No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1300.1821161901293025No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA1290.1807152963590771No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA1290.1807152963590771No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1260.17651261504840088No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA1220.17090903996749926No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1180.16530546488659764No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC1160.16250367734614685No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC1160.16250367734614685No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA1140.15970188980569602No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1140.15970188980569602No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1110.15549920849501983No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1080.1512965271843436No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT1080.1512965271843436No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT1070.1498956334141182No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG1020.1428911645629912No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT990.13868848325231498No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA980.13728758948208958No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT980.13728758948208958No Hit
GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAA980.13728758948208958No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT940.13168401440118796No Hit
GAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAA930.13028312063096256No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT910.12748133309051174No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT850.11907597046915933No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA820.1148732891584831No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA820.1148732891584831No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG820.1148732891584831No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTA800.11207150161803231No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG780.1092697140775815No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT780.1092697140775815No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA780.1092697140775815No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA760.10646792653713069No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA750.10506703276690528No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT720.10086435145622909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGAT200.003730157632.00000423
TGGGTTT400.00444310220.00000232
CCACTGC6200.013.41935422
CACTGCT6000.013.33333223
ACCACTG6400.013.25000121
AAGTACT850.001582706913.1764712
AAAGTAC850.001582706913.1764711
GATACCA6600.013.09090918
ACTGCTT6000.013.06666624
TACCACT6550.012.94656520
TGGAAGC4500.012.8000016
CTGCTTC5750.012.825
ATACCAC6750.012.819
TGCTTCC5550.012.68468526
GCTTCCA4200.012.57142827
ATGGAAG4600.012.5217395
CTTCCAT4100.012.48780428
TTGGGTT900.002432564512.44444531
TGATACC6950.012.43165517
TTGATAC7150.012.08391616
Produced by FastQC (version 0.11.5)